Project Assistant

Amrit Lamichhane

*** **** ** **, *******, Texas *7081; 832-***-****; lamichhaneam ***@*****.***

Chemist / Biotechnologist, seeking an entry level Environmental Chemistry position.

R esearch Skills/Techniques:

•

• • •

Media preparation, DNA Sequencing Wet chemistry analysis

• • •

Culture transfer DNA Analysis Gravimetry

• • •

Gel Electrophoresis Cell culture Gas chromatography

• • •

PCR Immunochemistry HPLC, GC MS, GC FID

• • •

Southern Blot ELISA LC MS-TOF

• • •

Western Blot Affinity chromatography Spectrometry

• • •

Northern Blot Immunohistochemistry Blast

• • •

D NA Extraction Primer designing Nomenclature,

• • p reparations, and

D NA Purification Molecular Cloning

• • reaction mechanisms of

Restriction Digests Site-directed

• a liphatic and aromatic

M utagenesis

Ligation Reactions

• Gel Electrophoresis

• compounds,

Protein Purification

• Titration

E ducation

M. S., Chemistry,

E xpected May 2015

University of Houston- Clear Lake (UHCL)

Houston, TX

B. S., Biotechnology, minor in Chemistry

December 2011

University of Houston- Downtown (UHD)

Houston, TX

Research P rojects

U H- Clear Lake, Dr. Chunlong Zhang

• Performing independent Research Project on LC MS -TOF Spring

2014 to present

Non-targeted an alysis of PPCPs in Wastewater T reatment Pl ant

U H- Downtown, Dr. Philip Lyons

• Performed independent Research on fungal ecology

Spring 2011

11 out of 30 experimented fungi were found to have l accase gene

Work Experience

Teaching Assistant, U H- Clear Lake, Houston TX

Fall 2013 to Present

• Assisted professor with Chemistry lab.

• Prepared lab for EPA audit, summer 2014

L ab Technician, QC laboratories, Houston TX

2012

• Measured the quality, strength and structural components of construction materials following CMET

C lass P rojects

• Completed a cell biology project involving insertion of human mitochondrial signal sequence into GFP

p lasmid and analyzed the f luorescence of protein in mitochondria

• Extracted alkaline phosphatase from the periplasmic space of Escherichia coli and quantified i t

• Coordinated a Service-learning project to teach science to elementary school children

P resentations

Texas branch of American Society of microbiology

M arch 2011

• Presented a poster t itled, “Identification of laccase genes from soil ascomyceta fungi”

P rofessional Organizations

• Student Member of American Society of microbiology

Since 2008

• Member of Leeuwenhoek Society, (Microbiology club),

2009 to present

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