Assistant Technician
Resume:
Natasha M. Rahmanizaker
Irvine, CA *****
*******.************@*****.***
EDUCATION
M.S. Applied Biotechnology (2014)
California State University, Los Angeles
Thesis: “Sterility test optimization on AmBisome.”
UC Extension Courses (2011-2012)
Advanced Microbiology, MICM 470 (University of California Los Angeles)
Advanced Molecular Biology, CMB 530 (University of California Riverside)
M.S. Microbiology, Magna Cum Laude (2008)
Azad University, Tehran
Thesis: “Isolation of fungi from fermented food and their therapeutic effect on bacilli.”
B.S. Cellular and Molecular Biology (2002)
Azad University, Tehran
EMPLOYMENT & PROFESSIONAL EXPERIENCE
Technical Service Intern (2013- 2014)
Gilead Sciences Inc., Technical Service Department, San Dimas, CA
Translated Gilead Sterility performance to a quick Laboratory screening setup with individual membranes.
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Measured relative adsorbed antibiotic residue by UV-Vis spectroscopy.
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Determined if antibiotic binding is effectively reduced with different rinsing fluids.
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Experimented with different pretreatment conditions to minimize antibiotic binding to the filter.
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Teaching Associate (2012-2014)
California State University, Los Angeles, College of Natural and Social Science
Micro202: Classic concepts in bacterial- host interactions to the students.
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Bio155: Principles of biology with an emphasis on cellular structure and function, and organic evolution.
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Bio100A: Biological principles as they apply specifically to animals.
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Bio 100B: Sub discipline of botany with the functioning, physiology and morphology of plants.
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Research Assistant (2010- 2011)
Florida Atlantic University, College of Medicine, Boca Raton, FL
• Explored the techniques that bring an innovative approach for increasing the effectiveness of future vaccines that
could also work well in smokers and those exposed to environmental tobacco.
• Determined the causal relationship between nicotine-induced DC alterations and host response to vaccines by
using flow cytometer for cell counting, cell sorting and analysis of the physical and chemical characteristics of
immune cells in smoker.
Research Assistant (2005-2010)
Azad University, Central Tehran Branch, Department of Chemistry, Tehran, Iran
• Synthesized the novel derivative of benzothiazole to use in new therapeutic application for tumor cell growth inhibition.
• Screened new banzothiazole toxicity and viability effects on two different human neuroblastoma cell lines.
Research Assistant (2005-2009)
Azad University Science and Research Branch, Tehran, Iran
• Isolated fungi from fermented food and their effect on B. Cereus and B. Subtilis.
• Examined products from fungi by Chromatography, Spectrometry and NMR method for screening the therapeutic active
ingredient in them.
• Studied natural probiotic in patients under antibiotic therapy by using ELISA, in vitro and in vivo.
Microbiology Lab Technician (2004-2005)
Laleh Hospital, Laboratory of Microbiology, Tehran, Iran
• Maintained laboratory culture collection and preserve cultures.
• Performed Environmental and Utility Monitoring in aseptic areas.
Research Assistant (2002-2004)
Pasteur Institute Laboratory of Biotechnology, Karaj, Iran
• Detection of Myco toxins from fungi in agricultural products by using Chloroform extraction.
• Isolation, characterization and sequencing of EPSPS gene from prokaryotic (E.coli) and eukaryotic.
Pathology Technician (2000-2002)
Mirdamad Clinical Laboratory, Tehran, Iran
• Performed laboratory tests on body tissues, cells and fluids from patients and industries.
BIOLOGY TECHNIQUES KNOWLEDGE
• Target Selection, transformation FACES • DNA sequencing
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& Bioinformatics Analysis on ELISA • Biocompatibility testing on
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Bacteria medical devices
NMR
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• Primer design and PCR • Micro titer dish biofilm
UV-Vis spectroscopy
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• Gel Electrophoresis formation assay
Molecular cloning
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• Protein purification SDS page
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• Tissue collection Flow cytometry
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COMPUETR KNOWLEDGE
• FASTA • SAP • Microsoft office (word, excel,
PowerPoint)
• BLAST • Microsoft project
PUBLICATIONS & PRESENTATION
The Synthesis of a New Phosphate Derivative of Benzothiazole and its Inhibiting Effect on two Series of Human
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Neuroblastoma Cells Growth, The Korean Journal of Chemical Engineering (2012)
Purification of Fungi Products with NMR and their effects on Bacilli, Caspian Journal of Internal Medicine, (2010).
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Isolation of fungi from fermented food and their effect on Bacilli. FEMS2009, Gothenburg, Sweden (2009).
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Pro biotic. Seminar (presented), Azad University, Science & Research Branch (2007)
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Effect of Antibiotics and Immune modulator agent on bacilli diarrhea patients. Sari University (2005)
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ACADEMIC COURSES
Advanced Cell Biology Survey on pharmacology & Mycology
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medical devices
Clinical trails and regulatory Graduate writing proficiency
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affair Biotechnology skill I/II Introduction to management
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Advanced Topics in Biology Animal tissue culture/Lab Project management
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Stem Cell Biology/Lab Microbiology I/II
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Molecular Biology & Bacteriology I/II
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Pharm/Toxicology Hematology
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Bioinformatics Ecology
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REFERENCES Available upon request
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