Manager High School

Desirée Denman

Research Scientist/Biology Instructor

Phone: 407-***-**** (mobile) 2602 Magnolia Point Cl

Email: acn6wu@r.postjobfree.com Panama City Beach, Fl 32408

Web: http://www.linkedin.com/in/desireedenman/

ABOUT

Life science graduate with academic credentials reinforced by industry experience at a stem cell banking and

genotyping facility and a molecular and cell biology laboratory.

Hands-on manager with three years of research experience, business and personnel/student training.

Passion for enhancing scientific understanding of neurological disorders by contributing to cellular research.

Able to leverage quantitative strengths to solve technical problems, advance scientific breakthroughs and support

innovative assay development.

ACCOMPLISHMENTS

Developed a protocol for isolating stem cells and generating a primary line from whole blood.

Started a stem cell banking and genotyping facility in Panama City, Panama and

Orlando Florida.

Research presented at the International Society for Stem Cell Research in

Yokohama, Japan

• Project: Commitment of Mesenchymal Stem Cells to Pre-adipocyes. June 2012. Patent pending.

TECHNICAL EXPERTISE

Molecular Biology Techniques

• Expression/molecular cloning, gel electrophoresis, macromolecule blotting and probing, Primer design, PCR, RT-

PCR, qPCR, in-vitro transcription, antibody staining, cDNA synthesis, DNA/RNA isolation, spectrophotometry,

chromatography, eukaryotic transfection, prokaryotic transformation, flow cytometry, SNP analysis, gene

identification/sequencing.

Microbiology Techniques

• Bacterial culture, staining, plate preparation, serial dilutions, plate counts, slide preparations, aseptic technique.

Cellular Techniques

• Eukaryotic suspension and adherent cell culture, growth media preparation, sterile technique, Class 2 BSC,

conditioned media preparation, stem cell isolations, cryopreservation. Cell Lines (all human): Glioblasoma

Multiforme stem cells, Neural stem cells, Mesenchymal stem cells (bone marrow, fat, blood), Pre-adipocytes, Induced

Pluripotent stem cells, Neuron-Committed Teratocarcinoma, Human Embryonic Kidney-293.

Microscopy Techniques

• Fluorescence microscopy, confocal microscopy, slide preparation, differential staining (whole blood and bacterial

cultures), immunocytochemistry, immunohistochemistry, fixation, permeabilization, sample mounting.

Software Skills

• Microsoft Word, Microsoft PowerPoint, Microsoft Excel, Pages, KeyNote, Numbers,Nikon Elements D, BioRad RT

PCR software, Carestream, Thermo scientific nanodrop, Molecular bio plate reader software, Lecia firecam and open

lab, PubMed, NCBI gene blast, Guava EasyCyte, Accuri c6 Flow, Illumina BeadXpress, GeneWiz.

WORK EXPERIENCE

Lake Mary High School Biology Teacher Lake Mary, FL

Contact: Keith McAuley (Assistant Principal) at 407-***-**** Aug 2013 - Jun 2014

• Taught freshman biology honors.

Desirée Denman

Research Scientist/Biology Instructor

• Duties include: Developing and implementing daily plans, wet and dry labs, individual and group work; analyzing

test data, evaluations and monitoring student progress; promoting an effective learning environment conductive to

enhancing a students education and the student/teacher partnership; participated in biology PLC meetings and parent

conferences.

Reason for leaving: Seeking a challenging new career opportunity.

Progenicyte/UCF Research Scientist/Lab Manager Orlando, FL

Contact: Tonia Patch (Lab Supervisor) at 321-***-**** Jan 2012 - Aug 2013

• Duties include: Keeping research projects on track, stem cell isolation, ordering supplies/equipment, record keeping,

personnel training, cell culture, iPSC generation, cellular transfection, PCR, target/novel gene identification/sequence.

Reason for leaving: Limited growth at company. Accepted a position teaching biology.

Stem Cell International Research Scientist Panama City, Panama

Contact: Tonia Patch (Lab Supervisor) at 321-***-**** Jul 2011 - Jan 2012

• Set up a stem cell and genotyping research centre at Punta Pacifica Hospital

• Duties include: Stem cell isolation, genotyping, ordering supplies/equipment, record keeping, personnel training,

cell culture, generating standard operating procedures (safety manuals, protocols), created patient documents (legal

forms, information packets).

Reason for leaving: Promoted to lab manager with sister company at Progenicyte

Progenicyte Research Assistant Orlando, FL

Contact: Tonia Patch (Lab Supervisor) at 321-***-**** Dec 2010 - Jul 2011

• Process patient samples and run experiments

• Duties include: Stem cell isolation, personnel training, cell culture, generating standard operating procedures

(safety manuals, protocols), PCR, sample processing, iPSC generation, cellular transfection, target gene

identification.

Reason for leaving: Promoted to Research Scientist with sister company at Stem Cell International

Undergraduate Research Orlando, FL

Contact: Tonia Patch (Lab Supervisor) at 321-***-**** Aug 2010 - Dec 2010

• Trained in stem cell maintenance, Immuno-histochemistry/-cytochemistry techniques, lab safety, FACS analysis,

cellular transfection.

Reason for leaving: Hired by Progenicyte to work for sister company, Stem Cell international.

EDUCATION

GRADUATED MAY 2011

University of Central Florida

B.S. Microbiology and Molecular Biology (Minor in Forensic Science)

B.S. Biotechnology (Minor in Health Sciences)

COMMUNITY INVOLVEMENT

Franklin’s Friends Volunteer Orlando, FL

http://www.franklinsfriends.info On-going

• Franklin's Friends is a local organization who's mission is to promote animal welfare and support animal charities

throughout Central Florida.

• Duties include: fund raising for various events, working events, bathing dogs, distributing flyers.

Contact this candidate