Technical Staff

Sunday Rittle

acn67y@r.postjobfree.com 267-***-****

SUMMARY

Staff Biologist with profound technical in vivo skills with focuses in

Neuroscience, Alzheimer's and Developmental & Reproductive Toxicology

research. Demonstrated strengths in quickly adapting to various work

environments, effective communication with both departmental and cross -

functional teams, and the ability to develop working relationships that

generate results. I bring a meticulous, detail oriented approach in

performing surgeries and leading multiple projects simultaneously.

Expertise includes:

Managed Timelines of Study Work Stereotactic Survival Surgeries

High Level of Planning and In Vivo Microdialysis

Organizational Skills Developing Animal Models

High Attention to Detail

PROFESSIONAL EXPERIENCE

Melior Discovery, Exton, PA February 2014-June 2014

Biologist

New Technical and Surgical Capabilities:

. PTZ seizure induced infusions in mice.

. 6-OHDA lesions in rats.

. Electrode Implantation/Surgeries for EEG recordings.

Merck & Co., Inc., West Point, PA 2002-2013

In Vivo Pharmacology, Staff Biologist 2005-2013

Proficiently supported the Neurochemistry efforts by conducting and

managing all in vivo aspects of the Microdialysis lab.

. Scheduled service calls for lab equipment, placed purchase orders

through SAP ordering system, and obtained specialized quotes.

. Established/validated new materials & equipment for all behavioral and

neurochemistry studies/surgeries.

. Planned and coordinated all microdialysis studies in lab, including:

. Ordered all rats through Animal Ordering System

. Managed timelines of internal and external vendors.

. Conducted and ran complex rat and mouse microdialysis studies.

. Presented analyzed data at numerous poster sessions, departmental and

project team meetings.

Implementation of New Assays and Technology:

. Enhanced lab productivity by setting up and validated new RATURN

systems.

. Managed, lead and independently coordinated all initiatives needed to

renovate a

new in vivo microdialysis lab. Initiatives included:

. Effectively communicated with the CAD team and engineers to

personally design an in vivo microdialysis lab, tissue

sampling/surgical suite, and drug prep area.

. Coordinated & scheduled work orders with cross departmental

contractors & LAR supervisors.

. Implemented all Safety, GLP and IACUC requirements for renovated

spaces.

Surgical and Technical Capabilities:

. Surgically implanted microdialysis probes into various brain regions

in both rats and mice, using aseptic techniques.

. Manipulated standard microdialysis and surgical protocols for special

applications, including:

. ICV infusion for drug delivery.

. Surgically implant dual microdialysis probes.

( Trained, mentored and was a technical resource for co-workers in

the following areas:

. Brain collection, CSF collection and histological probe

placements.

. Stereotactic surgeries and aseptic techniques.

. Various methods of sample collection and dosing.

. Microdialysis skills and study work.

Developed and validated new animal models to support Neuroscience research:

. IgG-192 cholinergic lesion model in Wistar Hanover and Sprague Dawley

rats.

( Intraventricular Toxin Injections, under various forms of

anesthia.

( Harvested brains delicately for Immunohistochemistry processing.

( Conducted ChAT and standard ELISA assays.

. Scopolamine mouse model with a partial cognitive deficit.

( Detected deficit with behavioral assays and reversing it with

compound.

( Collected cerebral spinal fluid and blood samples for PK

analysis.

( Conducted and optimized the Contextual Fear Conditioning

cognition assay.

( Performed brain homogenizations, ELISAs and Genotyping.

Developmental & Reproductive Toxicology, Staff Biologist 2002-2005

Developed technical skills needed for evaluating fetuses in skeletal,

cardiac, internal/external examinations and reproductive amoralities in a

GLP lab.

Proficiency in:

( Drug Preparation.

( Rat, Mouse and Rabbit IP, IV, SC and oral dosing.

( Rat and Mouse CSF collection

( Rat, Mouse and Rabbit terminal and non-terminal blood collections,

using various methods.

( Rat and rabbit C-sections and parental evaluations.

( Organized and performed GLP studies in compliance with protocols &

SOPs.

( Participated in internal audits for studies and data

analysis/compiling.

EDUCATION

BA, Neuroscience - with cum laude, University of Delaware, Newark, DE

Minor in Chemistry-University of Delaware, Newark, DE

PUBLICATIONS

Effects of the selective adenosine A2A receptor antagonist, SCH 412348,

on the parkinsonian phenotype of mitoPark mice. European Journal of

Pharmacology, 2014

Dual orexin receptor antagonists lower rat brain histamine levels without

lowering hippocampal acetylcholine, in process

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