Curriculum Vitae

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DAVID A. GOLDSTROHM, PH.D.

**** ********** ****, ********* *****, CO 80126, 970-***-****, acn5q8@r.postjobfree.com

CORE COMPETENCIES

• Bioanalytical chemistry laboratory manager at a leading renewable chemicals and advanced biofuels company.

o Developed, validated and implemented kinetic and HPLC-based enzymatic assays for renewable energy production.

o Included increasing productivity 3-fold, team management, assay design, robot handling/repair, LC-UV, SOP development,

report building.

• GLP-certified leader of small and large molecule biotherapeutics division at a leading bioanalytical CRO.

o LC-MS/MS quantitation and characterization of RNA and protein biotherapeutics (including peptides and mimetics).

o Included method development, validation, analyte isolation, detection and quantitation, instrument usage, report building, in-

phase/client audit evaluations, QC and QA support.

• Team leader of antioxidant knock-in mouse project at the number one respiratory research hospital in the world.

o Developed a novel antioxidant knock-in mouse model to better understand pulmonary and cardiovascular disease.

o Included enzymatic assays, protein biochemistry and immunohistochemistry, morphometry, animal husbandry, dissection,

molecular biology, data analysis and presentations of results to top tier journals and meetings.

• Team leader of interdisciplinary phosphoproteomics project at a leading academic institution.

o Designed novel mass spectrometric phosphoproteomic workflows to better understand complex human disease regulation.

o Included characterizing qualitative and quantitative protein changes (1057 phosphoproteins, 111 novel phosphorylation sites),

collaboration setup, workflow design, phosphoprotein/peptide enrichment, LC-MS/MS, immunoassays, data analysis and

database entry and presentations of results to top tier journals.

• Laboratory manager and safety officer of a leading gene regulation laboratory.

o Elucidated novel gene activation mechanisms for transcriptional regulators during oxidative stress.

o Included biochemistry, yeast genetics, cell and molecular biology protein expression/purification and protein-protein/protein-

DNA interactions.

TRAINING

• Senior Biochemist, Bioanalytical Chemistry, Gevo Inc., 2014-2015

Advisor: Steve McBryant, Bioanalytical Team Leader

Summary: Bioanalytical chemistry laboratory manager, kinetic and HPLC-based enzymatic assays

• Biologicals Senior Chemist, Pharmaceutical Division, Pyxant Labs Inc., 2013-2014

Advisor: John Begley, Director of Operations

Summary: GLP-certified leader of small and large molecule biotherapeutics, method development and validation

• Senior Research Fellow, Department of Medicine, National Jewish Health, 2010-2013

Advisor: Russell P. Bowler, M.D., Ph.D.

Summary: Team leader of antioxidant knock-in mouse project, pulmonary and cardiovascular disease regulation

• Postdoctoral Fellow, Department of Biochemistry, Colorado State University, 2006-2010

Advisor: Norman P. Curthoys, Ph.D.

Summary: Team leader of interdisciplinary phosphoproteomics project, metabolic acidosis disease regulation

• Research Assistant, Department of Biochemistry and Biophysics, University of Arizona, 1998-2000

Advisor: Michael A. Wells, Ph.D.

Summary: Laboratory researcher, in vivo amino acid metabolism

• Undergraduate Research Assistant, Department of Chemistry, University of Arizona, 1997

Advisor: Robin Polt, Ph.D.

Summary: Laboratory researcher, synthetic chemist for anti-cancer PDMP development

EDUCATION

• Doctor of Philosophy, Department of Biochemistry, Colorado State University, 2006

Advisor: Laurie A. Stargell, Ph.D.

• Master of Science, Department of Biochemistry, Colorado State University, 2002

Advisor: Laurie A. Stargell, Ph.D.

• Bachelor of Arts, Department of Biochemistry, University of Arizona, 2000

Advisor: Michael A. Wells, Ph.D.

9356 Pepperwood Lane • Highlands Ranch, CO 80126 • Phone: 970-***-**** • Email: acn5q8@r.postjobfree.com

Curriculum Vitae Page 2 of 4

SCIENTIFIC EXPERTISE

Laboratory

• Immunohistochemistry • Morphometry • ELISA • Enzymatic Assays

• Mass Spectrometry • Peptide Characterization • Protein Biochemistry • Gel Electrophoresis

• Q-TOF-LC-MS • Protein Characterization • Protein Purification • Sample Preparation

• LTQ RPLC-MSn • Peptide Enrichment • Molecular Cloning • RT-PCR

• MALDI-TOF/TOF • Protein Isolation • Chromatin IP • Animal Dissection

• API 4000 Q Trap • Amino Acid Analysis • Yeast Two-Hybrid • Primer Extension

• Data Interpretation • Protein Fractionation • Genetic Screens • Tissue Culture

• Bioinformatics • SDS-PAGE • Western-blotting • DNase I Footprint

• 2D-HPLC • Genetic Mutations • Pull-downs • S1 Nuclease

• Complex Mixtures • Cell Biology • Co-IP • Run-on

• Liquid/Solid Handling • Chromatography • Gene Knockouts • EMSA

Software

• Odyssey • Analyst 1.4.2 • SoftMaxPro • Mascot/SEQUEST

• ImageQuant • TOMTEC Quadra 3 • TECAN EvoWarePlus • Scaffold

• iCycler • Microsoft Office • Chromeleon 7.2 • Xcalibur

PROFESSIONAL DEVELOPMENT

Managing

• Laboratory manager for bioanalytical chemistry division, 2014-present.

• GLP-certified leader for small and large molecule pharmaceutical division, 2013-2014.

• GLP-certified biologicals senior chemist for small molecule pharmaceutical division, 2013-2014.

• Team leader for department of medicine pulmonary division antioxidant knock-in mouse initiative, 2010-2013.

• Group manager of phosphoprotein development project, 2006-2010.

• Yeast/biochemical laboratory manager including team leader, project design, and equipment repair, 2004-2006.

• Radiation manager including departmental training, record maintenance, waste disposal, and clean up, 2003-2006.

• Hazardous waste manager including departmental training, record maintenance, waste disposal, and clean up, 2006.

Consulting

• Method development, validation, data analysis and reporting for life science sponsors and clients, 2005-present.

Selected Collaborations

• Spearheaded network of 16 top tier research groups to develop novel models to treat cardiopulmonary disease resulting in

publication “Development of a novel R213G EC-SOD knock-in mouse model to analyze antioxidant function during oxidative

stress resulting in new advances to combat pulmonary and cardiovascular disease”, 2010-2014.

• Spearheaded collaboration with proteomics core facility resulting in publication “Optimized workflow for confident identification

of phosphopeptides using a linear ion trap mass spectrometer”, 2010.

• Developed experimental designs and provided useful discussion resulting in the publication “Histone chaperone specificity in

Rtt109 activation”, 2008.

• Performed protein purification and functional analysis resulting in the publication “The assembly of the preinitiation complex in

eukaryotic transcription: thermodynamic and kinetic characterization”, 2006.

• Provided critical reviews and helpful insight resulting in the publication “Disruption of histone deacetylase gene RPD3

accelerates PHO5 activation kinetics through inappropriate Pho84p Recycling”, 2005.

Teaching

• Principles of Biochemistry and Eukaryotic Cellular Biology Lecture, Colorado State University, 2002.

• Principles of Biochemistry and Eukaryotic Cellular Biology Lab, Colorado State University, 2003.

• Eukaryotic Cell Biology Lecture, Colorado State University, 2003.

9356 Pepperwood Lane, Highlands Ranch, CO 80126 • Phone: 970-***-**** • Email: acn5q8@r.postjobfree.com

Curriculum Vitae Page 3 of 4

Mentoring

• Trained and mentored 10 graduate students and 4 undergraduate students, Colorado State University, 2001-2010.

• Tutored graduate students in biochemistry, molecular biology, and genetics, Colorado State University, 2001-2005.

• Designed experimental protocols and managed booth to represent CSU biochemistry department at the National Western Stock

Show to support community involvement with science, 2006.

• Served on numerous mock oral examination committees, Colorado State University, 2002-2010.

HONORS AND AWARDS

Awarded 2nd Place Cash Award for outstanding poster presentation (FAMRI Conference, Miami, 2012).

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Awarded to Postdoctoral Research Day Planning Committee (University of Colorado Medical Campus, 2012).

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Awarded CCTSI certificate for CO-Mentor/Mentee completion (University of Colorado Medical Campus, 2011-2012).

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Preecha Kownin Memorial Cash Award for top research by a graduate student (Colorado State University, 2006-2007).

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Academic Honorable Mention (University of Arizona, 1997-2000).

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Summer of Excellence Award (University of Arizona, 1998).

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National Youth Leadership Forum on Medicine Award (San Francisco, 1998).

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Regent’s Scholarship Award (University of Arizona, 1997).

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SELECTED PROFESSIONAL ASSOCIATIONS

American Society of Biochemistry and Molecular Biology

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Colorado BioScience Association

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Protein Biochemistry and Biophysics

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American Association of Pharmaceutical Scientists

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Colorado Biotech and Pharma

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Molecular and Cellular Biology

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American Chemical Society

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Society of Free Radical Biology and Medicine

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Colorado Biological Mass Spectrometry Society

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American Society of Mass Spectrometry

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Biopharmaceutical Analysis

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SELECTED ORAL AND POSTER PRESENTATIONS

Goldstrohm, D.A., Oberley-Deegan, R.E., Weaver, M.R., Oury, T.D., Mason, R., Enghild, J.J., Bowler, R.P. Mechanisms by

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which the EC-SOD R213G SNP reduces the risk for lung disease (18th Society for Free Radical Biology and Medicine, Atlanta,

GA, 2011).

Pate, K., Goldstrohm, D.A., Oberley-Deegan, R.E., Regan, E., Crapo. Forced running elicits arthritis and oxidative stress in

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antioxidant impaired mice (18th Society for Free Radical Biology and Medicine, Atlanta, GA, 2011).

X. Bai, A.R. Ovrutsky, K. Takeda, A. Dakhama, J.M. Hartney, J.L. Matsuda, D. Ordway, E.W. Gelfand, T. Azam, L-Y. Chang,

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D.A. Goldstrohm, R.P. Bowler, C.A. Dinarello, E.D. Chan. Transgenic mice expressing human interleukin-32 in the lungs have

physiologic features suggestive of emphysema (54th Aspen Lung Conference, Aspen, CO, 2011).

X. Bai, S. Shang, D. Ordway, R. Basaraba, I. Orme, A. Ovrutsky, M. Kartalija, K. Takeda, A. Dakhama, J. Matsuda, E.W.

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Gelfand, T. Goldstrohm, D.A., Azam, L.-Y. Chang, C. Dinarello, E.D. Chan, Denver, Fort Collins, CO, p.A3336. Mice

Expressing Human Interleukin-32 (IL-32) In The Lungs Have Increased Lung Compliance And Are More Resistant To

Mycobacterium Tuberculosis (18th American Thoracic Society Conference, Denver, Colorado, 2011).

Gammelgaard, D., Goldstrohm, D.A., Curthoys, N.P., and Prenni, J.E. Proteomic analysis of protein phosphorylation in the renal

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response to metabolic acidosis (58th American Society of Mass Spectrometry Conference, Salt Lake City, Utah, 2010).

Goldstrohm, D.A., Gammelgaard, D., Broeckling, C.D., Prenni, J.E., and Curthoys, N.P. Mass spectrometric identification of

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changes in protein expression and phosphorylation that regulate the renal response to metabolic acidosis (57th American Society of

Mass Spectrometry Conference, Philadelphia, Pennsylvania, 2009).

Goldstrohm, D.A., and Stargell, L.A. Transcriptional activation during oxidative stress (Keystone Symposia, Regulation of

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eukaryotic transcription: from chromatin to mRNA, Taos, New Mexico, 2006).

Goldstrohm, D.A., and Stargell, L.A. Transcriptional activation during oxidative stress (Pingree Symposia, Colorado, 2006).

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9356 Pepperwood Lane, Highlands Ranch, CO 80126 • Phone: 970-***-**** • Email: acn5q8@r.postjobfree.com

Curriculum Vitae Page 4 of 4

Goldstrohm, D.A., and Stargell, L.A. Dynamic interplay of transcription factors during oxidative stress (Cellular and Molecular

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Biology Research Symposia, Fort Collins, Colorado, 2006).

Babre, M., Lee, S., Goldstrohm, D.A., and Stargell, L.A. Differing roles of Mediator subunits in response to stress (Pingree

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Park Symposia, Pingree Park, Colorado, 2006).

Goldstrohm, D.A., and Stargell, L.A. A transcriptional model for the war against oxidative stress (Cellular and Molecular

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Biology Research Symposia, Fort Collins, Colorado, 2004).

SELECTED PUBLICATIONS

Pate, K., Sherk V., Carpenter R., Weaver, M., Crapo, S., Gally F., Chatham, L., Goldstrohm, D.A., Crapo J., Kohrt W., Bowler,

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R., Oberley-Deegan R., and Regan, E. Mice with reduced extracellular superoxide dismutase in tissue experience cartilage

damage and less favorable bone adaptation in response to running. (Journal of Applied Physiology, 2015).

Goldstrohm, D.A., Lee, S.K., and Stargell, L.A. Mediator plays a role in post-preinitiation complex recruitment function during

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transcriptional activation during oxidative stress (Submitted: Journal of Biological Chemistry, 2015).

Hartney JM, Stidham T, Goldstrohm, D.A., Oberley-Deegan RE, Weaver MR, Valnickova-Hansen Z, Scavenius C, Benninger

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RK, Leahy KF, Johnson R, Gally F, Kosmider B, Zimmermann AK, Enghild JJ, Nozik-Grayck E, Bowler RP. A common

polymorphism in EC-SOD affects cardiopulmonary disease risk by altering protein distribution. (Circulation Cardiovascular

Genetics, 2014).

Gottfredsen R.H., Goldstrohm D.A., Hartney J.M., Larsen U.G., Bowler R.P., Petersen S.V.. The cellular distribution of

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extracellular superoxide dismutase in macrophages is altered by cellular activation but unaffected by the naturally occurring

R213G substitution (Free Radicical Biology and Medicine, 2014).

Goldstrohm, D.A. Determination of a non-steroidal anti-inflammatory drug in canine plasma by LC-MS/MS (Bioanalytical

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Method Validation Report, Pyxant Labs Inc., Study 2671, (1) 1-12, 2014).

Goldstrohm, D.A. Determination of a fluoroquinolone antibiotic in feline plasma by LC-MS/MS (Bioanalytical Method

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Validation Report, Pyxant Labs Inc., Study 2626, (1) 1-12, 2014).

Goldstrohm, D.A. Determination of a fluoroquinolone antibiotic in canine plasma by LC-MS/MS (Bioanalytical Method

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Validation Report, Pyxant Labs Inc., Study 2625, (1) 1-12, 2014).

Goldstrohm, D.A. Determination of a peptide therapeutic for hepatitis b virus infection in monkey plasma by LC-MS/MS

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(Bioanalytical Method Validation Report, Pyxant Labs Inc., Study 2521, (1) 1-10, 2014).

Goldstrohm, D.A. Determination of a peptide therapeutic for hepatitis b virus infection within dose formulations for injection

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(Bioanalytical Method Validation Report, Pyxant Labs Inc., Study 2598A, (1) 1-9, 2014).

Goldstrohm, D.A. Determination of a peptide therapeutic for hepatitis b virus infection in rat plasma by LC-MS/MS

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(Bioanalytical Method Validation Report, Pyxant Labs Inc., Study 2513, (1) 1-10, 2014).

Goldstrohm, D.A. Determination of a siRNA therapeutic for hepatitis b virus infection in rat plasma by LC-MS/MS

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(Bioanalytical Method Validation Report, Pyxant Labs Inc., Study 2512, (1) 1-12, 2013).

Goldstrohm, D.A. Determination of a siRNA therapeutic for hepatitis b virus infection in monkey plasma by LC-MS/MS

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(Bioanalytical Method Validation Report, Pyxant Labs Inc., Study 2520, (1) 1-12, 2013).

Goldstrohm, D.A., Broeckling, C.D., Prenni, J.E., and Curthoys, N.P. Importance of manual validation for the identification of

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phosphopeptides using a linear ion trap mass spectrometer (Journal of Biomolecular Techniques, 2011).

Kraemer, S.M., Goldstrohm, D.A., Berger, A., Hankey, S., Rovinsky, S.A., Moye-Rowley, S.W., and Stargell, L.A. TFIIA plays

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a role in the response to oxidative stress (Eukaryotic Cell, 2006).

Goldstrohm, D.A., Pennington, J.E., and Wells, M.A. The role of hemolymph proline levels as a nitrogen sink during blood

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meal digestion by the mosquito Aedes aegypti. (Journal of Insect Physiology, 2003).

REFERENCES

Steven J. McBryant, Ph.D. Shane Karnik, M.S. Russell P. Bowler, M.D., Ph.D Jessica E. Prenni, Ph.D. Laurie A. Stargell, Ph.D.

Team Leader Bioanalytical Director Bioanalytical Science Director of COPD Clinic Director of Proteomic and Professor

Gevo, Inc. Pyxant Labs National Jewish Health Metabolomics Facility Colorado State University

345 Inverness Drive, Suite 310 4720 Forge Rd, Suite 108 1400 Jackson Street, K715a 1870 Campus Delivery MRB #2021 1870 Campus Delivery MRB #379

Englewood, CO 80112 Colorado Springs, CO 80907 Denver, CO 80206 Fort Collins, CO 80523-2021 Fort Collins, CO 80523-1870

970-***-**** 719-***-**** 303-***-**** 970-***-**** 970-***-****

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