Research and Development Specialist

Dr. Aswati Subramanian

**** ******* *** ****, #* Oxford, Ohio 45056. 513-***-****. *******@*******.***

Objective: To obtain an industry position in laboratory management in an application oriented

research atmosphere.

Profile: Biologist with 13 years experience in laboratory research.

10 years research experience in cell culture and molecular biology techniques (DNA and RNA) along

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with strong background in protein biochemistry including instrument usage.

Published multiple research papers in peer reviewed and high impact factor journals in various research

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fields ranging from molecular biology to bioengineering.

Oral and poster presentations in international, national and local scientific meetings.

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Research group leader of undergraduate and graduate students involved in laboratory research.

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Expertise in performing microscopy, electron microscopy and teaching electron microscopy to graduate

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and undergraduate students from various research backgrounds.

Appointments- Research Associate:

1) Department of Chemical, Paper and Biomedical Engineering (January-May, 2014).

Growth of mesenchymal stem cells on scaffolds and scaffold fabrication techniques for bone tissue repair.

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2) Department of Biology (January 2014-present).

Role of microgravity on plant development in Arabidopsis thaliana.

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Ultra-structural analysis of muscle tissue in the Gray Catbrid (Dumetella carolinensis).

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Role of glia in remodeling of the adult nervous system in Drosophila melanogaster.

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Education:

Ph.D.: Dept. of Zoology, Miami University, Ohio (2007-2013).

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Graduate Assistant: Dept. of Zoology, Miami University, Ohio (2003-2006).

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Master of Science (Neuroscience): University Of Mumbai, India (2001-2003).

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Bachelor of Science (Life Sciences): University Of Mumbai, India (1998-2001).

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Laboratory and Computer skills (Techniques and Instrument usage):

Cell culture work: Culturing and maintaining E.coli, human and mouse and Tetrahymena cell lines.

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Polymer formulation for 3D bio-blotting of scaffolds for bone tissue engineering.

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Molecular biology and Bioinformatics: Gene cloning, PCR and primer designing, DNA and RNA

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isolation, restriction digestion, gel electrophoresis, sequencing and southern blotting, quantitative RT-

PCR, genetic transformation of Tetrahymena thermophila, peptide mass fingerprinting, analysis of LC-

MS/MS data, sequence alignment using BLAST.

Microscopy: Transmission and Scanning Electron Microscopy, light microscopy, fluorescence and

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confocal Microscopy-Biological sample preparation and instrument usage, and video microscopy.

Protein Biochemistry: Protein isolation, SDS- polyacrylamide gel electrophoresis, two-dimensional gel

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electrophoresis, MALDI-TOF and immunohistochemistry.

Computer skills: Working knowledge of Microsoft Office, image processing, analysis and video analysis using

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programs such as Image J, Image Pro-Plus, Adobe Photoshop, Corel Draw and GIMP.

Awards:

First Place, Image Category and Graduate Student Travel Award - Cell Dance Contest, American

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Society for Cell Biology, Annual Meeting, 2009.

Second Place-Poster Presentation, Ohio Miami Valley Chapter of Society for Neuroscience, University

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of Cincinnati, Ohio, U.S.A., 2004.

Graduate Research Assistantship- Cell, Molecular and Structural Biology, funded by a President’s

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Academic Enrichment Award, Miami University, Ohio, 2010.

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