Development Assistant
Resume:
Lida H. Kimmel
Chester, CT *****
Education:
M.S. Microbiology, 1988
University of Connecticut
Storrs, CT
B. S. Biology, 1986
State University of New York at Plattsburgh
Plattsburgh, NY
Experience:
December 2014-Current. Associate Scientist, Alexion
Pharmaceuticals
Cell culture of primary hepatocytes, IPSC derived hepatocytes
and cell lines for transfections with Moderna RNAs. Development
of in cell westerns on LiCor Odyssey as well as western blotting
on Protein Simple Imager. qPCR measurement of RNA from
transfected cells.
Jan 2012-November 2014. Research Scientist, Translational
Medicine, Kolltan Pharmaceuticals
Biomarker Assay Development on Meso Scale Discovery Platform to
support Phase 1 Clinical Studies for biologics targeting
tyrosine receptor kinases. Developed image based assays using
Cell Profiler software to measure tube formation and cell
scatter. Techniques used include tissue culture, FACS (Accuri),
western blotting and IHC.
2005-August 2011. Senior Scientist, Neuroscience Research Unit,
Pfizer Inc
Extensive use of a microscope based imaging system (Arrayscan,
Thermo-fisher) and application to CNS programs for drug
discovery. Image analysis algorithms applied to various types
of cell biology (neurite outgrowth, synapatogenesis, nuclear
translocation). Responsibilities included system oversight and
training new users. Biochemical techniques used include, SDS-
PAGE, western blot, immunoprecipitation, immunohistochemisty,
cloning, ELISAs, characterization of antibodies for
immunofluorescense and use of IPSCs for screening TRK B receptor
agonists. In vivo work related to small animal handling, dosing
of drugs and dissection of brain regions for isolation of
protein and RNA.
2000-2004. Scientist, Neuroscience Research Unit, Pfizer Inc
Molecular biological techniques used for cloning and expression
of mg quantities of proteins for high throughput screening in
bacterial and insect cell systems. Reagent validation and assay
development for drug screening.
1996-2000. Scientist, Pharmacogenomics and Clinical Biochemical
Measurements Group, Pfizer
Application of proteomics to identify biomarkers for use as
endpoints in CNS Clinical Development programs. Experience in
the evaluation of antibodies for development of ELISAs and assay
development. Analysis of samples from clinical studies in Head
Trauma, Alzheimer's disease and Depression with the goal of
finding surrogate markers for disease progression. Development
of a novel method for removing serum proteins from CSF samples
in preparation for 2D gel electrophoresis (patent application
PCT/GB99/01742).
1994-1996 Associate Scientist, Immunology, Pfizer Inc Cloning
and expression of genes in mammalian, bacterial and insect cell
expression systems. Protein purification and development of a
protein-protein interaction ELISA. Techniques include tissue
culture, molecular biology, protein purification, gel
electrophoresis, and western blotting.
1994 Associate Scientist, TargetTech Inc.
Optimization of gene expression in HepG2 cells using a receptor
mediated delivery system for treatment of Hepatitis B infection.
Laboratory techniques used with this position include isolation
of DNA, Southern analysis, slot/dot blots, luciferase assays,
tissue culture and virus isolation.
1990-1993 Research Associate, University of Connecticut Health
Center
Basic biochemical techniques were used to study the role of a
sperm protein in sperm-egg fusion. Techniques learned and
utilized for these projects include PCR, sequencing, isolation
of RNA/DNA, cloning, Southern, Northern and Western blots,
tissue culture, ascites production in mice, antibody
purification, in vitro fertilization, collection of gametes from
guinea pigs and mice, and florescence microscopy.
1988-1990 Research Assistant 111, University of Connecticut
Health Center
Research involved development of a selective medium for
isolation of Streptococcus mutans, analysis of well water for
fluoride and organized clinical trials of dental products.
Skills used include basic microbiology, maintenance of stock
cultures, identification and isolation of microorganisms.
Publications:
Robin J. Kleiman, Lida H Kimmel, Susan E. Bove, Thomas A Lanz, John
F.Harms, Alison Romegialli, Kenneth S. Miller, Amy Willis, Shelley des
Etages, Max Kuhn and Christopher J. Schmidt. Chronic Suppression of
Phosphodiesterase 10A Alters Striatal Expression of Genes Responsible
for Neurotransmitter Synthesis, Neurotransmission, and Signaling
Pathways Implicated in Huntington's disease. JPET, January 2011 vol.
336 no. 1 64-76.
Trey Sunderland, Nadeem Mirza, Karen T. Putnam, Gary Linker, Deepa
Bhupali, Rob Durham, Holly Soares, Lida Kimmel, David Friedman, Judy
Bergeson, Gyorgy Csako, James A. Levy, John J. Bartko and Robert M.
Cohen. Cerebrospinal fluid B-Amyloid 1-42 and tau in control subjects
at risk for Alzheimer's disease: The effect of APOE4 allele.
Biological Psychiatry, vol 56, no. 9, November, 2004.
Trey Sunderland, Gary Linker, Nadeem Mirza, Karen T. Putman, David L.
Firedman, Lida H.Kimmel, Judy Bergeson, Guy J. Manetti, Matthew
Zimmermann, Brian Tang, John J. Bartko and Robert Cohen. Decreased
Beta-Amyloid 1-42 and Increased Tau Levels in Cerebrospinal fluid of
Patients with Alzheimer's Disease. JAMA, 289, 2094-2103, 2003.
Young-Jie Zhou, Min Chen, Nancy A. Cusack, Lida H. Kimmel, Kelly
Magnuson, James Boyd, Wen Lin, Joseph L.Roberts, Andrea Lengi, Rebecca
H. Buckley, Robert L. Geahlen, Fabio Candotti, Massimo Gadina, Paul
Changelian, and John O'Shea. Unexpected Effects of FERM Domain
Mutations on Catalytic Activity of Jak3: Structural Implications for
Janus Kinases, Molecular Cell, Vol.8, 1-20, November 2001.
Min, C., A. Cheng, Y. Chen, A. Hymel, E. Hanson, L. Kimmel, Y. Minami,
T.Taniguchi, P. Changelian, and J. O'Shea. The amino terminus of Jak3
is necessary and sufficient for the binding of the common gamma chain
and confers the ability to transmit IL-2 mediated signals. PNAS,
1997:94:6910-6915.
Myles, D. G., L. Kimmel, C. Blobel, J. White and P. Primakoff.
Identification of a binding site in the disintegrin domain of fertilin
required for sperm-egg fusion. PNAS 1994:91:4195-4198.
Photo on cover of Nature, ICC of Guinea Pig Sperm, Vol 356, March 19,
1992
Lin, Y., L. Kimmel, Myles, D. G., and P. Primakoff. Molecular cloning
of the human and monkey sperm surface protein PH-20. PNAS,
1993:90:100**-*****.
Kimmel, L. and N. Tinanoff. A modified mitis salivarius medium for a
caries diagnostic test. Oral Microbiol Immunol 1991:6:275-279.
Keck, D., L. Kimmel, and N. Tinanoff. Fluoride in Connecticut Water
Supplies and Prescribing Dietary Fluoride Supplements. CT State
Dental Assoc. Journal, Fall 1990:30-33.
Continuing Education:
Cancer Biology, Brown University, fall '95
Cell Biology, Brown University, fall '97
Embryology, Brown University, fall '98
Neuropharmacology, Brown University, spring '99
Anatomy and Physiology, Capital Community
College, fall '11
Patents:
Affinity Depletion Method (OGS-Pfizer) application (5/99),
accepted
Method of Following CNS Diseases, application (6/2002)
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