project Assistant

CURRICULAM VITAE

Current Address: Permanent Address:

V. Shravani

D/O V. Venkata Narayana Reddy 2/20,Dondlavagu(vi,po)

#*/*,**** * *****, 13th main road, Lingala(M), kadapa(Di)

Kaggadaspura, C.V ramann nagar, Pincode no:516484

Behind Om Sakthi Temple, : +918*********.

Bangulore,Karnataka-94. @:******************@*****.***

CAREER OBJECTIVE:

Seeking a position to utilize my knowledge, skills and abilities in right place where, it

offers competitive environment, a true profession to put in the acquired knowledge and gain

exposure to upcoming technologies while being resourceful, innovative and flexible.

ACADEMIC CHRONICLE:

Education Board/ Place and Year of % of

University Institution passing marks

M.Sc. SVIMS SRI VENKATESWARA

[Biotechnology] UNIVERSITY INSTITUTE OF 2014 73.2%

TIRUPATI MEDICAL SCIENCES,

TIRUPATI

SRI

B.Sc. VENKATESWARA LOYOLA DEGREE

[Botany,Zoology,Chemistry] UNIVERSITY, COLLEGE, 2012 70.2%

TIRUPATI PULIVENDULA

BOARD OF SRI Y.S.S.R JUNIOR

Intermediate INTERMEDIATE COLLEGE, 2009 68%

[Bi.P.C] SIMHADRIPURAM

SSC BOARD OF NIVEDITHA

SECONDARY VIDYASALA HIGH 2007 83%

EDUCATION SCHOOL,

PULIVENDULA

M.Sc Dissertation

My Project Work Is On “Identification And Characterization Of Peptidyl Deformylase

From Staphylococcus Aureus ATCC12600” In Our Institution(“Sri Venkateswara

Institute Of Medical Sciences, Tirupati)

TECHNICAL PROFICIENCY:

Bio-chemical and Bio-physical techniques:

Spectrophotometric analysis of proteins and nucleic acids, Gel Filtration

Chromatography, Thin Layer & Paper Chromatography, Column Chromatography, Dialysis

Ammonium sulphate fractionation.

Molecular Biology Techniques:

Genomic DNA & RNA isolation, mRNA purification, Plasmid DNA isolation, PCR

(Polymerase Chain Reaction), AGE, SDS-PAGE, Protein isolation, purification and

characterization, restriction digestion analysis of DNA, Transformation of competent cells.

Enzyme technology:

Estimation of enzyme activity, determining the effect of pH, temperature, substrate

concentration, enzyme concentration, time on enzyme activity.

Biochemistry:

Determination of blood sugar, blood urea, total protein, serum creatinine, qualitative and

quantitative reactions of carbohydrates and precipitation reaction of proteins.

Immunological and microbiological techniques:

Blood grouping, double immuno diffusion, Rocket immuno electrophoresis, Dot

immunoblot assay, ELISA,Staininng of mirobes, isolation of microbes from water, spoiled food

and air, Screening of microbes by pour plate, spread plate and streak plate technique, serial

dilution, antibiotic sensitive test, growth curve of E.coli, effect of temperature, radiation, aeration

on growth of microbe, isolation of rhizobium, Aztobacter, Azospirrilum, staining of VAM,

Isolation of petroleum degradation microorganisms and xenobiotic compounds

Genetic engineering:

Cloning of DNA into expression vectors, transformation of microbes, genetic mapping by

transduction and conjugation

Cell Culture:

Primary culture, Trypsinization, Sub-culturing, Culture maintenance, Cryopreservation,

Cross-contamination validation, Culture characterization methods, Immunohistochemical (IHC)

and Immunocytochemical (ICC) staining, Expression analysis, MTT assay

Bio-statistical techniques:

Analysis of variance, Bar-chart & pie-chart, Chi-square

Instruments Handled:

Sonicator Homogenizer

PCR (Thermocycler) Centrifuges

Inverted Microscope Ultra centrifuges

CO2 Incubator Electronic balance

Gel Doc Autoclave

UV Spectrophotometers Colony counter

Calorimeters Hemocytometer

Incubators

Workshops &Seminars Attended:

4thNational Seminar on “COMPUTER AIDED DRUG DESIGN” organized by

department of Bioinformatics, Sri Venkateswara Institute of Medical Sciences

(SVIMS), Tirupati, Andhra Pradesh, India on during15-16, February, 2013.

5thNational Seminar on “BIOINFORMATICS” organized by department of

Bioinformatics, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati,

Andhra Pradesh, India on during15-16, February, 2014.

Other Skills:

Proficiency in handling MS Office, and Knowledgeable on internet operations.

PERSONAL PROFILE:

Name : V. Shravani

Father’s Name : V. Venkata Narayana Reddy,

Date of Birth : 03-06-1992

Gender : Female

Marital Status : Single

Nationality : Indian

Religion : Hindu

Category : OC

Languages Known : Telugu, English, and Hindi.

Hobbies : Watching T.V and Reading Books.

DECLARATION:

I hereby declare that the above mentioned information is true to my knowledge and I bear

the responsibility for the correctness of the mentioned particulars.

Date: 30-5-2014,

Place: TIRUPATI.

(V. Shravani)

sReference:

Dr. P.V.G.K Sarma

Associate professor and Head

Department of Biotechnology,

SVIMS, Tirupati.

Ph: +91-955*******.

Dr.C.Bhuvaneswaridevi,

Assistant professor

Department of Biotechnology,

SVIMS, Tirupati.

Ph:-087********,ext 2394,2395.

Contact this candidate