teaching faculty
Resume:
Curriculum Vitae
Mr. M. MANOJ PRABHAKAR, M.Sc., M.Phil., Ph.D
Melayakudi - (Post),
Paramakudi - (Taluk),
Ramanathapuram - (Dist),
Tamilnadu - 623 707.
India.
Email : *******.**@*****.***
Mobile : + 91-
CAREER OBJECTIVE
. To seek a position to utilize my skills and ability in my forth-coming
carrier that shows me as a new, dynamic and able person on the
respective field.
. To pursue a remarkable achievement in the platform of Medical
Biochemistry and biotech
ACADEMIC QUALIFICATIONS
Ph.D Biotechnology (2012 - 2014) [Thesis Submitted Waiting for VIVA]
Department of Biochemistry and Biotechnology, Faculty
of Science,
Annamalai University, Annamalainagar, Tamilnadu, India.
M.Phil., Biotechnology (2010 - 2012) [8.17 / 10, First class with
Distinction]
Department of Biochemistry and Biotechnology, Faculty
of Science,
Annamalai University, Annamalainagar, Tamilnadu, India.
M.Sc., Biotechnology (2005 - 2010) [8.02 / 10, First class with
Distinction]
(Integrated Course) Department of Biochemistry and Biotechnology,
Faculty of Science,
Annamalai University, Annamalainagar, Tamilnadu, India.
Higher Secondary (2004 - 2005) [92%, First class with Distinction]
A.V Hr. Sec. School, Paramakudi,
Tamilnadu, India.
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TITLE OF THE Ph.D RESEARCH WORK
In silico, in vitro and in vivo approaches to explore ferulic acid as
an anticancer agent.
TITLE OF THE M.Phil RESEARCH WORK
Chemopreventive potential of chrysin on 7,12-dimethylbenz[a]anthracene
(DMBA) induced skin carcinogenesis in Swiss albino mice.
TITLE OF THE M.Sc RESEARCH WORK
Protective effect of berberine on immunoexpression pattern of p53 and
bcl-2 in 7,12-dimethylbenz[a]anthracene (DMBA) induced skin carcinogenesis
in Swiss albino mice
SUMMARY OF THE DOCTORAL STUDY
The anticancer potential of ferulic acid was assessed by exploring the
proapoptotic and anti-cell proliferative potential through molecular
docking with target proteins. We observed that ferulic acid interacted with
target proteins with lowest binding energy forms a stable conformation. We
also observed a well hydrophobic interaction between ferulic acid and
target proteins demonstrates an effective ligand could modulate the
expression profiles of proapoptotic and cell proliferation cascade.
In vitro anticancer potential of feulic acid was assessed by analyzing
the cytotoxic anti-cell proliferative and proapoptotic potential in Hep-2
cancer cell lines. We observed that ferulic acid significantly inhibited
the cell viability followed by series of apoptotic events such as excess
ROS generation, nuclear damage and fragmentation, apoptotic cell death. We
also observed an altered expression profiles of apoptotic and cell
proliferative related proteins in ferulic acid treated cells. The
chemopreventive potential of ferulic acid in 7,12-dimethylbenz[a]anthracene
induced hamster buccal pouch carcinogenesis was investigated molecularly by
assessing the expression pattern of apoptosis and cell proliferative
proteins. We observed a significant modulation in the protein expression
profiles of ferulic acid treated groups. Thus the present study concludes
that ferulic acid has shown a potent anticancer activity in in silico, in
vitro and in vivo methodologies.
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AREA OF INTEREST
. Cancer chemoprevention - Biochemical and Molecular aspects
. Biochemistry
. Cell biology
. Molecular biology
. Pharmacology and Food Technology
. Clinical Biochemistry
RESEARCH EXPERIENCE
Animal Handling (Hamster, Wistar Rat, Sprague Dawley Rat and Mice)
. Cancer Biology (Cancer chemoprevention)
Tumor Induction
. Oral cancer - Golden Syrian Hamster model
. Skin cancer - Swiss albino mice
. Diabetes Mellitus
. Inflammatory disorders
BIOCHEMICAL ANALYSIS
> Biochemical, Spectrophotometric and Colorimetric analysis of lipid
peroxidation markers and antioxidants
> Enzyme Linked immuno Sorbent Assay (ELISA)
MOLECULAR BIOLOGY
> Total genomic DNA isolation
> Total RNA isolation
> Restriction digestion of genomic DNA
> Isolation of RNA and DNA proteins from animal tissues
> Immunohistochemistry
> SDS-PAGE and Western blotting
> Agarose gel electrophoresis,
> RNA and DNA methodology, Gel documentation,
> Polymerase chain reaction (PCR)
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COMPUTER SKILLS
* Proficient in MS-Office & Statistical Package for Social Sciences
(SPSS).
INSTRUMENTS HANDLED
* RT-PCR, Western blot apparatus, Gel documentation, High-speed
centrifuge, Gel Doc, Colorimeter, ELISA and Microscope.
ACHIEVEMENTS
. Awarded Endowment cash prize for Meritorious Students in M.Sc
(2009)
. Awarded Best Research Paper of the year in the Journal of Cell and
Tissue Research (JCTR) in 2010
. Awarded Research Fellow [JRF] from University Grants Commission
(UGC), New Delhi [2011 to 2013].
. Awarded Senior Research Fellow [SRF] from Indian Council of Medical
Research Grants Commission (ICMR), New Delhi [2013 to Till].
. Young Investigator Award for Paper Presentation in International
Conference held at Annamalai University (2013).
CONFERENCE/SEMINAR PAPER PRESENTED
INTERNATIONAL CONFERENCES
> Paper presented in 16th World Congress on Clinical Nutrition, Sep 11-
14, 2011, and 6th International Congress on Cardiovascular Diseases,
Sep 12-14, 2011, New Delhi.
> Participated in the CSIR-ICMR sponsored International Congress on
"Nutrition in Cardiovascular Diseases (Healthy Heart 10)" 15 & 16th
November 2010, in Department of Biochemistry & Biotechnology, Faculty
of science, Annamalai University, Chidambaram, Tamilnadu.
> Paper presented in the International Symposium on Innovations in Free
Radical Research and Experimental Therapeutics (IFRRET-2011) and 5th
Annual Convention of Association of Biotechnology and Pharmacy
organized by Department of Biotechnology, School of Biotechnology and
Health Sciences, Karunya University, December 7-9, 2011.
> Paper presented in the International conference on bioactive
phytochemicals and therapeutics (ICBPT) 5th to 7thApril 2013, in
Department of Biochemistry & Biotechnology, Faculty of science,
Annamalai University, Chidambaram, Tamilnadu
NATIONAL CONFERENCES
> Paper presented in 9th National Level Biological Congress on
"Biotechnology: Great Expectations & Achievements" 8th and 9th
January, 2010, Muthayammal College of Arts & Science, Rasipuram. Tamil
Nadu.
> Participated in National Conference on "Diabetes mellitus and Cancer"
(Dia-Can'09) 19 & 20th December 2009, in Department of Biochemistry &
Biotechnology, Faculty of science, Annamalai University, Chidambaram,
Tamilnadu.
> Participated in 20th National Conference of Indian Society of
Hypertension [BPCON-2010] 13 &14th November 2010, in Madurai
Tamilnadu.
> Participated in National conference on "Diabetes mellitus and Cancer"
(Dia-Can'10) 19 & 20th December 2010, in Department of Biochemistry &
Biotechnology, Faculty of science, Annamalai University, Chidambaram,
Tamilnadu.
> Participated in National Conference on " Explorations of the natural
bioactive compounds from the marine resources" (BIOACTIVA' 11) 10th &
11 th February 2011, Department of marine science, Bharathidasan
University, Tiruchirappalli.
> Participated in National Conference on "Diabetes mellitus and Cancer"
(Dia-Can'11) 19th December 2011, in Department of Biochemistry &
Biotechnology, Faculty of Science, Annamalai University, Chidambaram,
Tamilnadu.
> Participated in National Conference on Molecular and Cellular
Mechanisms of Diseases: Intervention with Natural Products & the 22 th
annual scientific meet of the society of biological chemists [INDIA],
Annamalainagar Chapter.
> Participated in National Conference on "Diabetes mellitus and Cancer"
(Dia-Can'12) 14-16th December 2012, in Department of Biochemistry &
Biotechnology, Faculty of Science, Annamalai University, Chidambaram,
Tamilnadu.
> Participated in National Conference on Trends in Medical
Biotechnology: Oppurtunities & Challenges, March 2012, in Department
of Biochemistry & Biotechnology, Faculty of Science, Annamalai
University, Chidambaram.
BOOK CHAPTER
Contributed as a coauthor in a book entitled "Oral cancer: An
avoidable and preventable cancer (ISBN:978**********) in the year 2012
published by Dr. S. Manoharan, Associated Professor, Annamalai University.
RESEARCH PUBLICATIONS
1. Manoj Prabhakar, M., Madavan Nirmal, R., Silvan, S., Baskaran, N.,
Muneeswaran, M. and Manoharan, S. Protective effect of berberine on
immunoexpression pattern of p53 and bcl-2 in 7,12-
dimethylbenz(a)anthracene induced skin carcinogenesis in swiss albino
mice J. Cell Tissue Res., 2010; 10: 2293-2297.
2. Murugaraj Manoj Prabhakar, Shanmugam Manoharan, Nagarethinam
Baskaran, Ramachandran Srinivasan, Sekar Karthikeyan, Shamsul Afaq
wani. Chemopreventive potential of chrysin in 7,12-
dimethylbenz(a)anthracene induced skin carcinogenesis in swiss albino
mice. Int. J. Res. Pharm. Sci.,2012; 3, 1-8.
3. Prabhakar MM, Manoharan S, Karthikeyan S, Rajasekaran D, Silvan S,
Baskaran N, Palanimuthu D. Proapoptotic potential of ferulic acid
during 7, 12-dimethylbenz(a)anthracene induced hamster buccal pouch
carcinogenesis. Int J Res Pharm Sci. 2012; 3: 622-627
4. Prabhakar MM, Vasudevan K, Karthikeyan S, Baskaran N, Silvan S,
Manoharan S. Anti-cell proliferative efficacy of ferulic acid against
7, 12-dimethylbenz(a) anthracene induced hamster buccal pouch
carcinogenesis. Asian Pac J Cancer Prev. 2012; 13: 5207-5211
5. Prabhakar MM, Manoharan S, Rejitharaji T, Selvasundaram R, Hairul
Islam VI. Ferulic acid reduces cell viability through its
apoptotic efficacy: An in vitro approach. Br J Med Med Res. 2015;
5(5): 612-621.
6. Manoharan S, Prabhakar MM. Adhatoda vasica Leaves Protect Cell Surface
Glycoconjugates Abnormalities During DMBA Induced Hamster Buccal Pouch
Carcinogenesis. Int J Pharmacog Phyto Res. 2014-15; 6(4).
7. Manoharan S, Rejitharaji T, Prabhakar MM, Karthikeyan S, Hairul
Islam VI. Apigenin reduces cell viability of Human epidermoid cancer
cells (Hep-2). Int J Res Pharm Sci. 2014; 5(3): 152-158.
8. Manoharan S, Rejitharaji T, Prabhakar MM, Manimaran A, Singh RB.
Modulating Effect of Ferulic Acid on NF-?B, COX-2 and VEGF Expression
Pattern During 7, 12- Dimethylbenz(a)anthracene Induced Oral
Carcinogenesis. Open Nutraceuticals J. 2014; 7: 33-38
9. Nagarethinam Baskaran, Shanmugam Manoharan, Simon Silvan, Duraisamy
Palanimuthu, Duraisamy Rajasekaran, Murugaraj Manoj Prabhakar, Sekar
Karthikeyan, Ramachandran Srinivasan and Shamsul Afaq Wani .Protective
effect of coumarin on cell surface glycoconjugates abnormalities
during 7,12-dimethylbenz(a)anthracene (DMBA) induced oral
carcinogenesis. Int J Biol Med Res. 2011; 2: 643-647
10. Silvan S, Manoharan S, Baskaran N, Anusuya C, Karthikeyan S, Prabhakar
MM.Chemopreventive potential of apigenin in 7,12-
dimethylbenz(a)anthracene induced experimental oral carcinogenesis.
Eur J Pharmacol. 2011; 670(2-3):571-7.
11. Simon Silvan, Shanmugam Manoharan, Nagarethinam Baskaran, Sekar
Karthikeyan and Murugaraj Manoj Prabhakar. Protective effect of
apigenin on 7,12- dimethyl benz(a) anthracene induced glycoconjugates
abnormalities in the plasma and buccal mucosa of golden syrian
hamsters Int J Pharm Sci Res. 2011; 7: 1753-1758.
12. Manoharan S, Singh A.K, Suresh K, Silvan S, Baskaran N, Manoj
Prabhakar M and karthikeyan S. Protective efficacy of andrographolide
on 7,12-dimethylbenz(a)anthracene induced genotoxicity in bone marrow
cells of golden syrian hamsters. Journal of Cell and Tissue Research.
2011; 11: 2751-2758.
13. Nagarethinam Baskaran, Shanmugam Manoharan, Sekar karthiyan,
Murugaraj Manoj Prabhakar. Chemopreventive potential of
coumarin in 7, 12- dimethylbenz[a]anthracene induced hamster
buccal pouch carcinogenesis. Asian Pac J Cancer Prev. 2012;13(10):5273-
9.
14. Manoharan S, Wani SA, Vasudevan K, Manimaran A, Prabhakar MM,
Karthikeyan S, Rajasekaran D. Saffron reduction of 7,12-
dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis.
Asian Pac J Cancer Prev. 2013;14(2):951-7.
SOCIAL SERVICE PROGRAMME
v Member in Bharat scouts (2002-2003), Don Bosco Matriculation Hr. Sec.
Sechool, Paramakudi-623707, Tamil Nadu, India.
v Member in National Service Scheme (NSS), Annamalai University, Tamil
Nadu, India and attended many service camps.
LANGUAGE KNOWN
v English (Read, Speak & Write)
v Tamil (Read, Speak & Write)
PERSONAL DETAILS
Name : M. Manoj Prabhakar
Father's Name : Mr. P. Murugaraj
Permanent Address : Melayakudi - Post,
Paramakudi - Taluk,
Ramanathapuram - Dist,
Tamilnadu, India - 623 707.
Gender : Male
Date of Birth : 31/03/1988
Martial status : Unmarried
Nationality : Indian
Contact Number : +91-975*******
E-mail : *******.**@*****.***
Declaration
I hereby declare that the above furnished details are true to my
knowledge.
Yours Sincerely
[M. Manoj Prabhakar]
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