Medical Assistant
Resume:
POONGUZHALI KAILASH, Ph.D (in short as PK)
**** ********** *****, ******, ** 27253
Cell: 952-***-**** Home: 952-***-****; **.*******@*****.***
Work Experience:
More than 20 years of teaching, research, and clinical lab experience in the field of biochemistry,
molecular biology, and analytical chemistry.
Teaching experience:
Sri Ramachandra Medical College and Research Institute (SRMC &RI) India – in collaboration
with Harvard University, USA. Please visit websites:
http://www.srmc.edu
http://www.sriramachandra.edu.in/university/library
http://news.harvard.edu/gazette/1997/04.17/HarvardMedicalI.html
Assistant Professor: 1988 – 2001 – Theory and labs
Bachelor’s degree- SRMC & RI- University
Medical Biochemistry : Medical students - Class size 200
Medical Biochemistry : Dental students - Class size 150
Medical Biochemistry : Pharmacy students – Class size 100
Medical Biochemistry : Physiotherapy students – Class size 50
Medical Biochemistry : Nursing students – Class size 50
Medical Biochemistry : Emergency students – Class size 50
Medical Biochemistry : Nutrition and genetics – Class size 25
Master’s degree – SRMC & RI – University
Medical Biochemistry : Graduate Medical students
Medical Biochemistry : Graduate dental students
Adjunct Professor : 2010-2013 – Theory and labs- Medical lab Science
Bachelor’s degree- St. Cloud State University (SCSU), Minnesota, USA
Organic chemistry – 3 Fall semesters
Medical Biochemistry – 3 Spring semesters
Clinical lab experience: USA
Team Lead and Charge supervisor – Labcorp, Burlington, NC 2013-Present
Team Lead and Charge supervisor – Viromed lab (Labcorp)- MN, 2012-2013
Senior Technologist – Viromed lab ( subsidiary of labcorp), MN, 2007- 2012
Clinical lab experience: India
Biochemist: 3000 bed hospital ( SRMC & RI ) – 1988- 2001
Lab Supervisor: 200 bed hospital (Child’s Trust hospital), India – 1987-1988
Research experience: USA
Assistant Scientist – University of Minnesota – 2002-2007
Co-Investigator: Observational Clinical Research Projects:
• Young Adults: Longitudinal Trends in Antioxidant (YALTA) - This study is a prospective
longitudinal cohort study that will evaluate a series of oxidative pathways and biomarkers
of various antioxidants by ELISA, HPLC, spectrophotometer, and liquid scintillation counter
in the CARDIA population.
• Combined Antioxidant and Preeclampsia Prediction Studies (CAPPS) - The aim of this
study is to extract and analyze antioxidants (Vitamins A, E, C) from the CAPPS cohort by
HPLC and to evaluate the relationship between blood antioxidants and preeclampsia.
• The Women’s Angiographic Vitamin and Estrogen (WAVE)-This study will examine the
effect of hormone therapy on coronary atherosclerosis in women and their role in oxidative
stress by analyzing various antioxidants.
• Epidemiology of Cancer in a Cohort of Older Women: Iowa Women’s Health Study- This
study will evaluate risk factors of several cancers in women. A major focus is the risk
factors for breast cancer by analyzing a dense set of the most common genetic variants in
the human genome, single nucleotide polymorphisms (SNPs).
Research experience: India
Investigator: Preclinical studies
• A study of biochemical markers for cartilage & other tissues destruction & effects of
tenoxicam & cynodon dactylon on the repair process of experimentally induced
osteoarthritis in guinea pigs
• Effects of ripe kernel juice of plantain (musa species) in experimental hyperoxaluric rats.
Education:
Ph.D. Biochemistry, Medical University, Chennai, India.
M.Phil Biochemistry, Madras University, Chennai, India.
M.S Biochemistry, Madras University, Chennai, India.
B.S Biochemistry, Madras University, Chennai, India.
Skills,Techniques, and Software:
• Molecular biology techniques including genotyping (SNP’s), multiplex, PCR, RFLP, SDS-
PAGE, agarose electrophoresis, blotting, transfection, transformation (Beckman-biomek
instrument, invader assay, sequenom)
• Training in molecular biology techniques- DNA, RNA extraction, transfection,
transformation, hybridization, blotting and cloning, PCR and sequencing.
• Experience in genomics lab in screening cystic fibrosis by high throughput instrumentation
and concepts of Bioinformatics.
• HPLC and GC-MS- Preparation, extraction, and analysis of biological samples (Vitamins
A, E, C) using High Performance Liquid Chromatography (HPLC) and isoprostanes by
GC-MS.
• Quantitative measurement of biomarkers of oxidative stress including Myeloperoxidase,
superoxide dismutase, ICAM, P-selectin, and oxidized LDL using ELISA
• Paraoxonase and platelet aggregating factor (PAF) using spectrophotometer and liquid
scintillation
• Estimation of collagenase, hydroxyproline, collagen, sialic acid, hexosamine, hexuronic
acid, beta glucuronidase, cathepsin D, and N-acetyl glucosaminidase in connective
tissues using spectrophotometer.
• Ability to work in a fast-paced environment with vast experience in automated ELISA
using, extraction of nucleic acids by Qiagen MDX, Roche Magnapure etc.
• Trainer for techs in various assays in infectious diseases laboratory and a good resource
in various aspects of the tests including problem solving and troubleshooting of high fast
paced automated instrumentations.
• High throughput automated instruments like CAPTAQ (Roche), Auto lipa (Siemens),
Genprobe (Hologic) Tigris for Chlamydia and Gonorrhea, molecular testing and
interpretation of various infectious diseases like HIV, HCV, HBV for clinical trials and
clinical samples.
• Strict follower of GDP, GCP, GMP, GLP and knowledge in FDA regulations, CLIA, NY state,
CAP as well.
• Tissue culture, microbiological and cell cycle phases by Flow cytometry and analyzing
data using cell quest pro software.
• Animal experiments (rats and guinea pigs model) with tube feeding, dissection,
biochemical analysis in various tissues, blood, and urine.
• Working knowledge of Cell Quest Pro, SOFTMAX Pro.
Publications- Authored and co-authored (13 publications both National and International)
Oral and poster presentations – both national and International.
1. Bharat Thyagarajan, David R. Jacobs, Jr, J. Jeffery Carr, Ogechika Alozie, Michael W.
Steffes, Poonguzhali Kailash, Jennifer H. Hayes and Myron D. Gross. Clinical Chemistry
54: 738-746, 2008. Factors Associated with Paraoxonase Genotypes and Activity in a
Diverse, Young, Healthy Population: The Coronary Artery Risk Development in Young
Adults (CARDIA) Study.
2. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Journal of
Clinical Biochemistry & Nutrition. 24 (3), 141-146, 1998.Effect of Cynodon dactylon and
Tenoxicam on the lysosomal enzyme activities in the cartilage tissue of osteoarthritis
guinea pigs.
3. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Journal of Indian
Rheumatism Association. 5, 89-90, 1997. Estimation of sialic acid in normal and
osteoarthritis guinea pigs.
4. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Asia Pacific
League of Associations for Rheumatology. 1, 92-95, 1997. Different stages of
experimental induction of osteoarthritis by intra- articular injection of papain in the animal
model of guinea pig.
5. G .Nalini, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, P. Kailash. Asia Pacific
League of Associations for Rheumatology. 1, 212-221, 1998. Effect of Type II collagen on
Adjuvant arthritis.
6. G. Nalini., H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, P. Kailash. Journal of
Clinical Biochemistry & Nutrition. 23, 77-83, 1997. The antioxidant status in Rheumatoid
arthritis.
7. P.Kailash and H.Chegu. British Journal of Urology. 74, 23-25, 1994. The influence of
banana stems extract on urinary risk factors for stones in normal and hyperoxaluric rats.
8. G.Nalini, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, P. Kailash. Journal of Indian
Rheumatism Association. 2 (3), 124-126, 1994. Urinary excretions of glycosaminoglycans
in rheumatoid arthritis.
9. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Biomedicine.
14, 45-48, 1994. Serum malondialdehyde levels in patients with systemic rheumatic
diseases.
10. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G Nalini. Biomedicine. 14,
49-51, 1994. Serum sialic acid in systemic rheumatic diseases.
11. G.Nalini, H.Chegu, A.N.Chandrasekaran, G.Chandrakasan, P. Kailash. British Journal of
Rheumatology. 32, No 12, 118-119, 1993. A comparative study of serum deaminases in
systemic rheumatic diseases.
12. G.Nalini, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, P. Kailash. Biomedicine. 2,
25-27, 1993. Serum biochemical markers in rheumatoid arthritis
13. P.Kailash and P.Varalakshmi. Indian Journal of Experimental Biology. 30, 440-442, 1992.
Effect of banana stem juice on biochemical changes in liver of normal and hyperoxaluric
rats
Abstracts:
B.Thyagarajan, C.Lessard, P. Kailash, J. Hayes, T.Mori, K. Anderson, M.Gross.
Comparison of DNA repair between cultured lymphocytes and fresh lymphocytes: The
alkaline unwinding flow cytometry assay- Proceedings of American Association of cancer
Research: April 2006.
Gross MD, Thyagarajan B, Kailash P, Hayes JH, Khaliq W, Anderson KE, Veltri G, Jacobs
DR. A Comparison of DNA Repair Activity in Several Lymphocyte Preparations; The
recovery of lymphocytes from cryopreserved whole blood and measurement of their DNA
repair activity. Proceedings of American Association of cancer Research: 2005.
P. Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, R.Nalini. The
chondroprotective effect of Cynodon dactylon on altered proteoglycan structure. Journal of
bone and mineral metabolism: 2003.
Oral and poster presentations:
P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. .Effect of cynodon
dactylon and tenoxicam on the lysosomal enzyme activities in the connective tissues of
osteoarthritic guinea pigs - Indian Association of Biomedical Scientists- Defense Institute
of Physiology and Allied sciences, Delhi, India,1997.
P.Kailash, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, G. Nalini. Serum
malondialdehyde in systemic rheumatic diseases - XIII Annual conference of Indian
Association of Biomedical Scientists- Trivandrum, Kerala State, India, 1992.
P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Effect of Cynodon
dactylon on lipid peroxides in cartilage tissue of osteoarthritis induced guinea pigs - Indian
Association of Biomedical Scientists - Kasturba Medical College, Mangalore, Karnataka
State, India, 1998.
P.Kailash .Women in science - Tuberculosis Research Centre, India, 1998.
P.Kailash, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, G. Nalini. Biochemical
markers of osteoarthritis - International satellite symposium collagen: Structures, Biology
and Technology, CLRI, India, 1994.
P.Kailash, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, G. Nalini. Different stages
of osteoarthritis-an animal model - International conference of the Indian Rheumatism
Association Iracon East West, India, 1994.
P.Kailash, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, G. Nalini. Serum sialic acid
in systemic rheumatic diseases - Annual conference of Society of Biological Chemists -
Centre for Cellular and Molecular Biology, Hyderabad, Andra Pradesh State, India, 1992.
Editor: Assistant Editor for Biomedicine Journal, 2000-2001
Professional Membership:
American Association of Cancer Research, 2006-2007
American Society of Biochemistry and Molecular Biology, 2002-2003
Indian Association of Basic Medical Scientists, lifetime member
Awards:
• Teaching award- Shared hopes and shared excellence- SRMC & RI – 1999
• ‘Go for Greatness’ award for Team work for consecutive two years 2010 and 2011 –
Viromed laboratory, Minnetonka, MN..
• Scholar -in– training award- The study of lysosomal enzymes in the synovial fluid of
osteoarthritic patients- July 1998.
• Scholar-in-training award- The status of pro-oxidant and anti-oxidant in cases of alcoholic
hepatopathies-July 1997.
Guest Lectures:
“Quality Assurance” in the CME program. October 6, 1997, at SRMC & RI, India.
“Effect of cynodon dactylon on isolated chondrocyte cells- an invitro study” in the National
conference on the scope of siddha medicine in the new Millennium, July 15-16, 2000.
References:
1. Patricia Ellinger MSEd, MLS (ASCP) CM, SBB
Medical Lab Science Program Director
**********@************.***
Cell: 612-***-****
2. Louise Millis MS, MLS (ASCP)
St.Cloud State University
Associate professor Biology
*******@************.***
3. Dr. Mohammad Mahroof-Tahir Ph.D.
Professor and Chairperson, Department of Chemistry
Saint Cloud State University
********@************.***
Cell: 320-***-****
http://web.stcloudstate.edu/mmahroof
4. Nathan S. Winter, Ph.D.
Professor of Chemistry
*******@************.***
Tel : 320-***-****
5. Lakshmaiah Sreerama Ph.D.
Professor of Chemistry
*********@************.***
6. Bharat Thyagarajan, Ph.D. M.D
Lab Director
********@***.***
Tel: 612-***-****
7. Nalini Ganesan Ph.D.
Professor of Biochemistry
Sri Ramachandra Medical College & Research Institute, India
**********@*****.***
Tel: 011 91-944*******
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