POONGUZHALI KAILASH, Ph.D (in short as PK)

**** ********** *****, ******, ** 27253

Cell: 952-***-**** Home: 952-***-****; acgtja@r.postjobfree.com

Work Experience:

More than 20 years of teaching, research, and clinical lab experience in the field of biochemistry,

molecular biology, and analytical chemistry.

Teaching experience:

Sri Ramachandra Medical College and Research Institute (SRMC &RI) India – in collaboration

with Harvard University, USA. Please visit websites:

http://www.srmc.edu

http://www.sriramachandra.edu.in/university/library

http://news.harvard.edu/gazette/1997/04.17/HarvardMedicalI.html

Assistant Professor: 1988 – 2001 – Theory and labs

Bachelor’s degree- SRMC & RI- University

Medical Biochemistry : Medical students - Class size 200

Medical Biochemistry : Dental students - Class size 150

Medical Biochemistry : Pharmacy students – Class size 100

Medical Biochemistry : Physiotherapy students – Class size 50

Medical Biochemistry : Nursing students – Class size 50

Medical Biochemistry : Emergency students – Class size 50

Medical Biochemistry : Nutrition and genetics – Class size 25

Master’s degree – SRMC & RI – University

Medical Biochemistry : Graduate Medical students

Medical Biochemistry : Graduate dental students

Adjunct Professor : 2010-2013 – Theory and labs- Medical lab Science

Bachelor’s degree- St. Cloud State University (SCSU), Minnesota, USA

Organic chemistry – 3 Fall semesters

Medical Biochemistry – 3 Spring semesters

Clinical lab experience: USA

Team Lead and Charge supervisor – Labcorp, Burlington, NC 2013-Present

Team Lead and Charge supervisor – Viromed lab (Labcorp)- MN, 2012-2013

Senior Technologist – Viromed lab ( subsidiary of labcorp), MN, 2007- 2012

Clinical lab experience: India

Biochemist: 3000 bed hospital ( SRMC & RI ) – 1988- 2001

Lab Supervisor: 200 bed hospital (Child’s Trust hospital), India – 1987-1988

Research experience: USA

Assistant Scientist – University of Minnesota – 2002-2007

Co-Investigator: Observational Clinical Research Projects:

• Young Adults: Longitudinal Trends in Antioxidant (YALTA) - This study is a prospective

longitudinal cohort study that will evaluate a series of oxidative pathways and biomarkers

of various antioxidants by ELISA, HPLC, spectrophotometer, and liquid scintillation counter

in the CARDIA population.

• Combined Antioxidant and Preeclampsia Prediction Studies (CAPPS) - The aim of this

study is to extract and analyze antioxidants (Vitamins A, E, C) from the CAPPS cohort by

HPLC and to evaluate the relationship between blood antioxidants and preeclampsia.

• The Women’s Angiographic Vitamin and Estrogen (WAVE)-This study will examine the

effect of hormone therapy on coronary atherosclerosis in women and their role in oxidative

stress by analyzing various antioxidants.

• Epidemiology of Cancer in a Cohort of Older Women: Iowa Women’s Health Study- This

study will evaluate risk factors of several cancers in women. A major focus is the risk

factors for breast cancer by analyzing a dense set of the most common genetic variants in

the human genome, single nucleotide polymorphisms (SNPs).

Research experience: India

Investigator: Preclinical studies

• A study of biochemical markers for cartilage & other tissues destruction & effects of

tenoxicam & cynodon dactylon on the repair process of experimentally induced

osteoarthritis in guinea pigs

• Effects of ripe kernel juice of plantain (musa species) in experimental hyperoxaluric rats.

Education:

Ph.D. Biochemistry, Medical University, Chennai, India.

M.Phil Biochemistry, Madras University, Chennai, India.

M.S Biochemistry, Madras University, Chennai, India.

B.S Biochemistry, Madras University, Chennai, India.

Skills,Techniques, and Software:

• Molecular biology techniques including genotyping (SNP’s), multiplex, PCR, RFLP, SDS-

PAGE, agarose electrophoresis, blotting, transfection, transformation (Beckman-biomek

instrument, invader assay, sequenom)

• Training in molecular biology techniques- DNA, RNA extraction, transfection,

transformation, hybridization, blotting and cloning, PCR and sequencing.

• Experience in genomics lab in screening cystic fibrosis by high throughput instrumentation

and concepts of Bioinformatics.

• HPLC and GC-MS- Preparation, extraction, and analysis of biological samples (Vitamins

A, E, C) using High Performance Liquid Chromatography (HPLC) and isoprostanes by

GC-MS.

• Quantitative measurement of biomarkers of oxidative stress including Myeloperoxidase,

superoxide dismutase, ICAM, P-selectin, and oxidized LDL using ELISA

• Paraoxonase and platelet aggregating factor (PAF) using spectrophotometer and liquid

scintillation

• Estimation of collagenase, hydroxyproline, collagen, sialic acid, hexosamine, hexuronic

acid, beta glucuronidase, cathepsin D, and N-acetyl glucosaminidase in connective

tissues using spectrophotometer.

• Ability to work in a fast-paced environment with vast experience in automated ELISA

using, extraction of nucleic acids by Qiagen MDX, Roche Magnapure etc.

• Trainer for techs in various assays in infectious diseases laboratory and a good resource

in various aspects of the tests including problem solving and troubleshooting of high fast

paced automated instrumentations.

• High throughput automated instruments like CAPTAQ (Roche), Auto lipa (Siemens),

Genprobe (Hologic) Tigris for Chlamydia and Gonorrhea, molecular testing and

interpretation of various infectious diseases like HIV, HCV, HBV for clinical trials and

clinical samples.

• Strict follower of GDP, GCP, GMP, GLP and knowledge in FDA regulations, CLIA, NY state,

CAP as well.

• Tissue culture, microbiological and cell cycle phases by Flow cytometry and analyzing

data using cell quest pro software.

• Animal experiments (rats and guinea pigs model) with tube feeding, dissection,

biochemical analysis in various tissues, blood, and urine.

• Working knowledge of Cell Quest Pro, SOFTMAX Pro.

Publications- Authored and co-authored (13 publications both National and International)

Oral and poster presentations – both national and International.

1. Bharat Thyagarajan, David R. Jacobs, Jr, J. Jeffery Carr, Ogechika Alozie, Michael W.

Steffes, Poonguzhali Kailash, Jennifer H. Hayes and Myron D. Gross. Clinical Chemistry

54: 738-746, 2008. Factors Associated with Paraoxonase Genotypes and Activity in a

Diverse, Young, Healthy Population: The Coronary Artery Risk Development in Young

Adults (CARDIA) Study.

2. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Journal of

Clinical Biochemistry & Nutrition. 24 (3), 141-146, 1998.Effect of Cynodon dactylon and

Tenoxicam on the lysosomal enzyme activities in the cartilage tissue of osteoarthritis

guinea pigs.

3. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Journal of Indian

Rheumatism Association. 5, 89-90, 1997. Estimation of sialic acid in normal and

osteoarthritis guinea pigs.

4. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Asia Pacific

League of Associations for Rheumatology. 1, 92-95, 1997. Different stages of

experimental induction of osteoarthritis by intra- articular injection of papain in the animal

model of guinea pig.

5. G .Nalini, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, P. Kailash. Asia Pacific

League of Associations for Rheumatology. 1, 212-221, 1998. Effect of Type II collagen on

Adjuvant arthritis.

6. G. Nalini., H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, P. Kailash. Journal of

Clinical Biochemistry & Nutrition. 23, 77-83, 1997. The antioxidant status in Rheumatoid

arthritis.

7. P.Kailash and H.Chegu. British Journal of Urology. 74, 23-25, 1994. The influence of

banana stems extract on urinary risk factors for stones in normal and hyperoxaluric rats.

8. G.Nalini, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, P. Kailash. Journal of Indian

Rheumatism Association. 2 (3), 124-126, 1994. Urinary excretions of glycosaminoglycans

in rheumatoid arthritis.

9. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Biomedicine.

14, 45-48, 1994. Serum malondialdehyde levels in patients with systemic rheumatic

diseases.

10. P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G Nalini. Biomedicine. 14,

49-51, 1994. Serum sialic acid in systemic rheumatic diseases.

11. G.Nalini, H.Chegu, A.N.Chandrasekaran, G.Chandrakasan, P. Kailash. British Journal of

Rheumatology. 32, No 12, 118-119, 1993. A comparative study of serum deaminases in

systemic rheumatic diseases.

12. G.Nalini, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, P. Kailash. Biomedicine. 2,

25-27, 1993. Serum biochemical markers in rheumatoid arthritis

13. P.Kailash and P.Varalakshmi. Indian Journal of Experimental Biology. 30, 440-442, 1992.

Effect of banana stem juice on biochemical changes in liver of normal and hyperoxaluric

rats

Abstracts:

B.Thyagarajan, C.Lessard, P. Kailash, J. Hayes, T.Mori, K. Anderson, M.Gross.

Comparison of DNA repair between cultured lymphocytes and fresh lymphocytes: The

alkaline unwinding flow cytometry assay- Proceedings of American Association of cancer

Research: April 2006.

Gross MD, Thyagarajan B, Kailash P, Hayes JH, Khaliq W, Anderson KE, Veltri G, Jacobs

DR. A Comparison of DNA Repair Activity in Several Lymphocyte Preparations; The

recovery of lymphocytes from cryopreserved whole blood and measurement of their DNA

repair activity. Proceedings of American Association of cancer Research: 2005.

P. Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, R.Nalini. The

chondroprotective effect of Cynodon dactylon on altered proteoglycan structure. Journal of

bone and mineral metabolism: 2003.

Oral and poster presentations:

P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. .Effect of cynodon

dactylon and tenoxicam on the lysosomal enzyme activities in the connective tissues of

osteoarthritic guinea pigs - Indian Association of Biomedical Scientists- Defense Institute

of Physiology and Allied sciences, Delhi, India,1997.

P.Kailash, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, G. Nalini. Serum

malondialdehyde in systemic rheumatic diseases - XIII Annual conference of Indian

Association of Biomedical Scientists- Trivandrum, Kerala State, India, 1992.

P.Kailash, H.Chegu, A.N Chandrasekaran, G.Chandrakasan, G. Nalini. Effect of Cynodon

dactylon on lipid peroxides in cartilage tissue of osteoarthritis induced guinea pigs - Indian

Association of Biomedical Scientists - Kasturba Medical College, Mangalore, Karnataka

State, India, 1998.

P.Kailash .Women in science - Tuberculosis Research Centre, India, 1998.

P.Kailash, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, G. Nalini. Biochemical

markers of osteoarthritis - International satellite symposium collagen: Structures, Biology

and Technology, CLRI, India, 1994.

P.Kailash, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, G. Nalini. Different stages

of osteoarthritis-an animal model - International conference of the Indian Rheumatism

Association Iracon East West, India, 1994.

P.Kailash, H.Chegu, A.N. Chandrasekaran, G.Chandrakasan, G. Nalini. Serum sialic acid

in systemic rheumatic diseases - Annual conference of Society of Biological Chemists -

Centre for Cellular and Molecular Biology, Hyderabad, Andra Pradesh State, India, 1992.

Editor: Assistant Editor for Biomedicine Journal, 2000-2001

Professional Membership:

American Association of Cancer Research, 2006-2007

American Society of Biochemistry and Molecular Biology, 2002-2003

Indian Association of Basic Medical Scientists, lifetime member

Awards:

• Teaching award- Shared hopes and shared excellence- SRMC & RI – 1999

• ‘Go for Greatness’ award for Team work for consecutive two years 2010 and 2011 –

Viromed laboratory, Minnetonka, MN..

• Scholar -in– training award- The study of lysosomal enzymes in the synovial fluid of

osteoarthritic patients- July 1998.

• Scholar-in-training award- The status of pro-oxidant and anti-oxidant in cases of alcoholic

hepatopathies-July 1997.

Guest Lectures:

“Quality Assurance” in the CME program. October 6, 1997, at SRMC & RI, India.

“Effect of cynodon dactylon on isolated chondrocyte cells- an invitro study” in the National

conference on the scope of siddha medicine in the new Millennium, July 15-16, 2000.

References:

1. Patricia Ellinger MSEd, MLS (ASCP) CM, SBB

Medical Lab Science Program Director

acgtja@r.postjobfree.com

Cell: 612-***-****

2. Louise Millis MS, MLS (ASCP)

St.Cloud State University

Associate professor Biology

acgtja@r.postjobfree.com

3. Dr. Mohammad Mahroof-Tahir Ph.D.

Professor and Chairperson, Department of Chemistry

Saint Cloud State University

acgtja@r.postjobfree.com

Cell: 320-***-****

http://web.stcloudstate.edu/mmahroof

4. Nathan S. Winter, Ph.D.

Professor of Chemistry

acgtja@r.postjobfree.com

Tel : 320-***-****

5. Lakshmaiah Sreerama Ph.D.

Professor of Chemistry

acgtja@r.postjobfree.com

6. Bharat Thyagarajan, Ph.D. M.D

Lab Director

acgtja@r.postjobfree.com

Tel: 612-***-****

7. Nalini Ganesan Ph.D.

Professor of Biochemistry

Sri Ramachandra Medical College & Research Institute, India

acgtja@r.postjobfree.com

Tel: 011 91-944*******

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