Maintenance Technician

Tetyana Zhelay, PhD

**** *** *****, ***#***, Toledo, OH 43614

419-***-****; email: ******@*****.***

OBJECTIVE: To obtain a scientist position within an industry setting where dedication to

work, research and such skills as molecular biology techniques, cell culture techniques, calcium

imaging and whole-cell patch-clamp recordings will be utilized.

EDUCATION

1995– 2000 Master of Science in Biophysics.

Taras Shevchenko National University, Kiev, Ukraine

Thesis title: Effects of Hypericum perforatum on sodium currents of rat

hypothalamic neurons

2000 – 2005 Doctorate in Philosophy in Biophysics and Neurophysiology

Dept. General Physiology of Nervous System

O.O. Bogomoletz Institute of Physiology of the National Academy of

Sciences (NAS), Kiev, Ukraine

Dissertation Title: Dihydropyridine-sensitivity for endogenous and recombinant

T-type calcium channels.

PROFESSIONAL EXPERIENCE

2000 – 2006 Junior Research Scientist

Department of General Physiology of Nervous System,

Bogomoletz Institute of Physiology.Kiev, Ukraine.

• Research focused on pharmacological effects of dihydropyridines:

nifedipine, nimodipine and nitredipine in rat thalamic neurons.

• Examined subtypes of endogenous low-voltage calcium channels and T-

type calcium channels expressed in Xenopus oocytes.

• Additional research focused on characteristics of stationary activation of

low-voltage calcium channels and their blocking intensity.

2006 – 2012 Postdoctoral Research Fellow

Department of Neurosciences

Raymond & Beverly Sackler Laboratory for Neuroendocrine Tumor Research,

University of Toledo College of Medicine, Toledo, Ohio

• Research focused on the linkage of voltage- and store-operated calcium

channel activation to neuroendocrine tumor cell dynamics.

• Identification of calcium entry pathways in a variety of human

gastroenteropancreatic cell lines.

• Additional research focused on novel calcium signaling pathways in breast

cancer and non-transformed intestinal and oral epithelia.

TECHNICAL SKILLS

• Whole-cell patch-clamp/ voltage-clamp recordings in isolated cells.

• Fluorescent microscopy with an emphasis on calcium imaging.

• Monitoring calcium dynamics following flash photolysis of novel caged compounds

• Nucleic acid extraction and purification and identification by RT-PCR and Real Time

PCR.

• Western blot analysis of protein

• Immunoassay techniques, ELISA

• Cell migration assays.

• Organ slice model preparation.

• In vitro cell culture maintenance.

• Isolation, maintenance and injection of Xenopus oocytes.

• Preparation of thalamic neurons (latero-dorsal nucleus) for patch-clamp experiments.

• Statistical analysis (Origin, Prizm, Igor Pro)

PUBLICATIONS (peer-reviewed)

1. T.I. Zhelay, S. Arunachalam, P. Goswamee and D.R. Giovannucci. The role of ORAI1

channels in store-operated calcium entry and calcium oscillations in human neuroendocrine tumor

cell lines. (manuscript in progress)

2. R.A. Ali, T.I, Zhelay, C. Trabbic, T.F. Walseth, J.T. Slama, D.R. Giovannucci and K.A. Wall.

Activity of nicotinic acid substituted nicotinic acid adenine dinucleotide phosphate (NAADP)

analogs in a human cell line: differences in specificity between human and sea urchin NAADP

receptors. Cell Calcium (submitted).

3. T.I. Zhelay, S. Zahedi and D.R. Giovannucci. (2012) An oxidant-based strategy targeting

carcinoid cancer cells. Chemotherapy (in revision).

4. S. Arunachalam, T.I. Zhelay, and D.R. Giovannucci. Molecular and functional

characterization of non-voltage operated Ca2+ entry pathways in gastrointestinal neuroendocrine

tumor cells, Int J Physiol Pathophysiol Pharmacol 2010;2(2):125-136).

5. T.I. Zhelay (2005). Nimodipine and nitrendipine effect on fast and slow components of LVA

Ca2+ channels. Neurophysiology 37 (1), 3-10.

6. A.K. Scheglovitov, T.I. Zhelay, Y. Vitko, V.N. Osipenko, E. Perez-Reyes, P.G. Kostyuk and

Y.M. Shuba (2005). Contrasting the effects of nifedipine on subtypes of endogenous and

recombinant T-type Ca2+ channels. Biochemical Pharmacology 69, 841-854.

7. T.I Zhelay and A.K. Scheglovitov (2004). Effects of nifedipine on voltage-regulated low-

threshold Ca2+-channels in rat thalamic neurons. Neurophysiology 36 (3), 193-203

8. A.K. Scheglovitov, T.I. Zhelay, A.P. Kondratzkiy, V.G.Najdenov and Y.M. Shuba (2004).

Nifedipine blocking mechanisms on three subtypes of Ca2+ channels (T-type). Comparative

analysis. Neurophysiology 36 (2), 101-110.

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