Engineering Chemical

AMANDA LEILA QUIGLEY B.Sc. (Hons) M.Sc. Ph.D.

**** ******* **** ***. ****, San Antonio, Texas 78240 Mobile: 956-***-****

Email: ******.*******@*****.*** D.O.B. 11/2/1984 Nationality: British

EDUCATION

2008- 2013 Ph.D. in Chemical Engineering, Imperial College London

Thesis Title: “Protein Aggregation Behaviour and the Second Virial Coefficient ”

2006 - 2007 M.Sc. Biochemical Engineering, University College London

Thesis Title: “Bioprocess Design for Glucosamine Production”

2003 - 2006 B.Sc. (Hons) 2:1 Biotechnology, University College London

Thesis Title: “Construction of a PapE Concatamer for use in Atomic Force Microscopy”

Design Project Title: “Plasmid DNA Vaccine Production for an Avian Flu Pandemic”

1996 – 2003 Tasker Milward V.C. School

2003 A-levels: 4 A-B grades achieved

2001 GCSEs: 6 A* and 5 A grades achieved

PROFESSIONAL EXPERIENCE

Application Scientist, deltaDOT Ltd., September 2013 – Present

Responsible for all aspects of a £126K Technology Strategy Board funded project in

collaboration with University College London to develop a novel platform for the rapid

screening of stability of protein formulations (includes technical research, project

management and reporting, finances and exploitation). Other responsibilities include

providing bio-analytic pre-sale services using deltaDOT’s proprietary High Performance

Capillary Electrophoresis (HPCE) system, and post-sales technical training, support and

trouble shooting.

Research Associate, Imperial College London, August 2013 – April 2014

A postdoctoral feasibility study investigating crystallization of industrial monoclonal

antibodies using nanotemplates as a potential tool for the rapid purification of proteins

during downstream bioprocessing.

Postgraduate Student, Imperial College London, July 2008 – January 2013

Ph.D. research focussing on protein-protein interactions quantified via the osmotic second

virial coefficient (B22). This parameter is a fundamental physiochemical property that

describes intermolecular interactions between particles (proteins) in solution. Investigations

probed the potentially significant role of B22 in describing the molecular processes that lead

to aggregation and crystallization in solution. The aim of this work was to develop new

analytical tools for the screening of proteins in aqueous solutions that are accurate and

efficient in the prediction of protein aggregation propensity.

Postgraduate Fellow, Parliamentary Office of Science and Technology, April – July 2012

A 3 month secondment funded by the Biotechnology and Biological Sciences Research

Council (BBSRC) for Ph.D. students to gain experience working in science policy. The

Parliamentary Office of Science and Technology (POST) is the UK Parliament's in-house

source of independent analyses of public policy issues related to science and technology.

Sub-warden, Fisher Hall, Imperial College London, March 2009 – September 2012

Sub-warden’s work as part of a team to assist in the running of the undergraduate Halls of

Residence, particularly with regard to pastoral care, emergencies, social activities and

discipline. I was also specifically in charge of accounting for the Hall’s finances (~£20K

budget).

SKILLS

Laboratory Skills: Protein Purification, Centrifugation, HPLC/FPLC (Ion-Exchange,

Hydrophobic Interaction, Affinity and Size Exclusion), Dynamic Light Scattering, UV and

fluorescence spectrometry, Nanoparticle Tracking Analysis, Quartz Crystal Microbalance,

Protein Quantification Assays, Protein Immobilization, SDS-PAGE, Western Blotting, ELISA,

Capillary Electrophoresis (capillary gel electrophoresis and capillary zone electrophoresis),

Crystallization Screening and Optimization (high throughput liquid handling, hanging drop,

sitting drop, batch), Optical Microscopy.

Teaching Skills: Supervision of laboratory and pilot plant sessions (weekly) at Imperial

College London for undergraduate Chemical Engineering students and grading papers (2007-

2009).

Project Management Skills: Experimental design, research, data analysis (including

statistical analysis), report writing and presentation, organisation of conferences/events.

Language Skills: Native English Speaker. Intermediate level of written and spoken Spanish.

Other Skills: Full current clean UK driving licence, First Aid at Work Qualification (OFQUAL

Level 3), Fire Prevention and Fire Safety at Work Qualification (Imperial College London).

HONORS AND AWARDS

Parliamentary Office of Science and Technology, Policy Internship Award, 2012

IChemE Biochemical Engineering Subject Group, Travel Bursary Award, 2012

Bioprocess UK, Conference Bursary Award, 2010 and 2011

Biotechnology and Biological Sciences Research Council, Ph.D. Studentship in collaboration

with FujiFilm Diosynth, 2007-2012

Biotechnology and Biological Sciences Research Council, M.Sc. Studentship, 2006-2007

Chevron International, REACH Undergraduate Scholarship, 2003-2006

PUBLICATIONS

Quigley, A., Heng, J. Y. Y., Liddell, J. M., Williams, D. R., “The Accurate Measurement of

Second Virial Coefficients using Self-Interaction Chromatography: Experimental

Considerations” European Journal of Pharmaceutics and Biopharmaceutics, 85 (2013) pp

1103-1111

Quigley, A., Williams, D.R. “Second Virial Coefficients as Determined using Self Interaction

Chromatography and Protein Aggregation in Solution” Biophysical Journal, 102 (2012) pp

442A, Published Conference Abstract

Quigley, A., Bunn, S., “Drug Resistant Tuberculosis” POSTnote 416 (2012)

Quigley, A., Williams, D.R., “The Second Virial Coefficient as a Predictor of Protein

Aggregation Propensity” Manuscript in Preparation

Quigley, A., Williams, D.R., “Similar-Interaction Chromatography of Proteins: A Novel Cross-

Interaction Chromatography Approach” Manuscript in Preparation

PROFESSIONAL MEMBERSHIPS

Associate Member, Institute of Chemical Engineers (IChemE), 2006-Present

Committee Member, IChemE Biochemical Engineering Special Interest Group, 2011-2013

Member, Biophysical Society, 2012

Member, Bioprocessing Research Industry Club, 2007-2012

Student Representative, Biochemistry and Biotechnology Undergraduate Course, University

College London, 2003-2006

REFERENCES

Dr. Daryl Williams (PhD Supervisor) Dr. John Liddell (Industrial Supervisor)

Department of Chemical Engineering Head of Process and Analytical Sciences

Imperial College London Fujifilm Diosynth Biotechnologies

London SW7 3BG Billingham TS23 1LH

*.*.********@********.**.** ****.*******@**********.***

+44 (0-20-759*-**** +44 (0-164*-******

Nigel Stokes (Managing Director) Dr. Dan Bracewell (External Examiner)

deltaDOT Ltd. University College London

The London Bioscience Innovation Centre 5.06 Bernard Katz Building

2 Royal College Street Gordon Street

London NW1 0NH London WC1H 0AH

*******@********.*** *.*********@***.**.**

+44 (0-20-769*-**** +44 (0-20-767*-****

APPENDIX

CONFERENCES AND MEETINGS ATTENDED

Bioprocess Briefing, September 2014, University College London, oral presentation

European Congress on Biotechnology, July 2014, Edinburgh, poster presentation

New Horizon Research, February 2014, EPSRC Centre London, oral presentation

MIBio 13, October 2013, Cambridge, poster presentation

Biophysical Society Meeting, 25-9 February 2012, San Diego, USA, poster presentation

Biopharmaceutical Analytical Group Meeting, 12 February 2012, Slough

Bioprocess UK Conference, 30 November-1 December 2011, Glasgow, poster presentation

Biochemical Engineering Young Researchers Meeting, 13-14 September 2011, London, oral

presentation

Modern Challenges in Therapeutic Protein Production, 11 June 2011, London

Chemical Engineering PhD Symposium, 29 March 2011, Imperial College London, oral

presentation

BRIC Dissemination Event, 22-23 March 2011, Warwick

Bioprocess UK Conference, 24-25 November 2010, Manchester, poster presentation

Biochemical Engineering Young Researchers Meeting, 13-14 April 2010, Cambridge

Protein Stability Conference, July 2009, Breckenridge, Colorado, poster presentation

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