Customer Service Technician

MARIAM VANETSYAN

**** ******** **** ****** ****, CA 90066 • Cell: 310-***-**** • *********@*****.*** •www.linkedin.com/pub/mariam

vanetsayn/89/946/598/

Professional Summary

Competent and Dedicated Histotechnologist, HTcm, with over 13 years of hands on clinical and research laboratory

experience, specialized in diagnostic and prognostic oncology. Well experienced in multiplatform

Immunohistochemistry/ISH/ICC assays as well as GLP proficient in various areas of laboratory tasks and Histology skills.

Processed and validated cell blocks that were used as controls for various immunoassays. Perfected Collection and Fixation

procedure specific to cell blocks, which substantially improved cell morphology and thus precision and accuracy of staining

results, resulting in faster TAT and decreased cost of operation.

Skills

•HT (ASCP) CM • Multiplatform IHC/ICC/ISH •Microtomy/Embedding•QA Microscopy•Sterile Technique

•Microarray Cell Blocks•Tissue Culture • Cytopreps• Excellent Customer Interaction Skills

•Hard working and energetic. Not afraid to be challenged and take on new tasks and acquire new skills.

•Detail oriented, organized, great at multitasking, time efficient and a good team player.

•Always strive to improve time and cost efficiency and quality of work.

•Strong technical knowledge and computer skills.

•Fluent in Armenian and Russian.

Professional Experience

Histotechnologist, 10/2014 Currently

Kaiser Permanente North Hollywood

Perform microtomy of various biopsied/autopsied paraffin blocks

Section over 100 blocks per day

Histotechnologist, 10/2012 to 11/2013

LabCorp – Irvine, CA

Performed multiplatform IHC/ISH and associated microscopy

Antibody titrations, inventory and parallel testing

Managed control block inventory and microtomy

Introduced cell blocks for IHC and ISH control use

Helped optimize staining procedure for HPV ISH platform change

Facilitated work flow of daily cases and with troubleshooting of staining deficiencies

Sr. Technologist, 12/2005 to 10/2012

Genzyme Genetics – Los Angeles, CA

Maintained and harvested tumor cell line cultures as well as managed a cryopreserved bank of over 500 various tumor lines.

Helped with experimental design and collected data for a study targeting enzymatic effect on certain surface antigens of cell

lines used as controls in IHC assays

Prepared and validated multipiece cell blocks for IHC/FISH/ISH positive control use for laboratory sites in

Los Angeles and New York

Helped save operating cost by providing in house cell block controls to FISH laboratory

Procured patient breast and prostate tissue for combined multipiece control blocks; as well as for HPV ISH

Optimized cell morphology and thus staining quality and intensity of cell blocks

Trained technologists in tissue culture techniques

Revised SOPs and logbooks

Facilitated development of log books for monitoring culture growth, harvest, cell block prep./testing as well as QA and QC

logs

Performed HPV ISH testing w/ manual probe application

Corporate Cell Lines Technician, 05/2001 to 12/2005

Genzyme Genetics – Los Angeles, CA

Maintained growth of various cancer cell lines as well as media preparations monitored growth/confluence and

cryopreservation

Ordered and started cultures of new cell lines for subsequent testing and validation as control blocks.

Aided in developing a systematic methods of record keeping for QA of grown cultures.

Ordered all the culture media and supplements as well as preparation of buffers and sterilization

Performed seeding of cancer cells @ concentrations 1:1mil. or 1:500,000 or 1:250,000 or higher into patient BM samples

for optimizing various Ab. titer testing as well as cancer cell positive enrichment stains

Performed harvest of cultured cells and subsequent processing of fixed pellets into cell blocks.

Performed ICC assays of blood and bone marrow aspirates for detection of MRD cells.

Lab Technician, 06/2000 to 05/2001

IMPATH, Inc. Los Angeles, CA

Processing of blood and bone marrow samples to isolate white blood cells.

Cytopreparation and Immunocytochemistry, which included daily use and prep. of Ab.

Clonogenenic ICC assay.

Freezing of cytopreped slides.

Miltenyi® positive enrichment for Ab. tagged cells.

Qualifications

HT(ASCP)CM

California State University at Long Beach, Long Beach, CA pursue BS in general biology (1995 1999)

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