Assistant Professor -Microbiology

Dear Sir/Madam,

I am pleased to submit my application for the position of Assistant

Professor -Microbiology. I completed my Ph.D. in Microbiology at Dr. Ram

Manohar Lohia Avadh University, Faizabad, Uttar Pradesh in 2012 under the

direction of Prof. S.K. Garg. My primary areas of specialization are

microbiology, biotechnology, enzymology and molecular biology.

My research interest has focused on production, purification and

characterization of microbial alkaline proteases. Utilizing the

results/data gathered during my doctoral research, I have published 8

research papers in various journals of international repute and high impact

factor and two manuscripts are in pipeline. I also monitored equipment

performance and maintenance schedules. I was involved in designing and

drafting of various research projects, submitted by our department to

various Indian funding agencies.

Currently, I am working as Senior Executive (Microbiology Division) at

Titan Biotech Limited, Bhiwadi, Rajasthan and hence have a good knowledge

of process development, literature survey and writing, lab management,

quality systems, cGMP norms, ISO and other international guidelines,

testing as per USP and/or other pharmacopeial procedures. I bear good

technical communication and presentation skills. I have a good experience

of designing experiments and analysis.

Please find with this letter of application a copy of my detailed C.V.

Please contact me if I can provide any additional materials that might help

the committee to evaluate my qualifications.

I appreciate your consideration of my application.

Sincerely,

Dr. Sanjay Kumar Singh

CURRICULUM VITAE

SANJAY KUMAR SINGH

Village and Post - Kataiya

District - Pratapgarh-230137

Uttar Pradesh, India

Tel. +91-945*******, 823-***-****

Email - *********@*****.***

Objective

Looking for a suitable teaching position in the field of Life Sciences, to

contribute efficiently with my learning's and admirable aptitude for

effective teaching and overall development of students with accuracy.

Academic Qualifications

Doctoral Research: Carried out at the Department of Microbiology at Dr. Ram

Manohar Lohia Avadh University, Faizabad, UP, India, under

supervision of Prof. S. K. Garg.

Research Title: Production, purification and molecular characterization of

serine protease from potential bacterial isolate.

Examination Institute/University Subject(s) Year % Marks

Ph.D. Dr. Ram Manohar Microbiology 2012 Not

Lohia Avadh Applicable

University, Faizabad

Post-gradua Dr. Ram Manohar Microbiology 2006 67.2

tion Lohia Avadh

(M.Sc.) University, Faizabad

Graduation Dr. Ram Manohar Chemistry, 2004 74.4

(B.Sc.) Lohia Avadh Botany, Zoology

University, Faizabad

Intermediat Govt. Inter College Physics, Biology, 2001 65.6

e Pratapgarh Chemistry, English

(U.P.Board) & Hindi.

High School Govt. Inter College English, Hindi, 1999 60.66

Pratapgarh Social Science,

(U.P.Board) Mathematics,

Science & Biology.

Other Qualifications

Qualification University / Year % Marks

Institute

CSIR-UGC NET CSIR 2012 Not applicable

Professional Experience:

Organization Designation Duration

Titan Biotech Limited, Senior Executive 17/07/12 to Till date

Bhiwadi, Rajasthan (Microbiology Division)

Company Profile: Titan Biotech Limited (ISO 9001:2008, 22000:2005, cGMP,

FSSAI certified) is one of the leading manufacturers & exporters of

the biological media bases, dehydrated culture media, plant tissue culture

media, chemicals, reagents, kits, etc., having strong performance in

domestic and international markets. Titan products have a large application

in Microbiology, Pharmaceuticals, Nutraceuticals, Food & Beverages,

Biotechnology & Fermentation, Cosmetics, Veterinary & Animal Feeds,

Agriculture Industries, etc.

Responsibilities

. Leading team of microbiologists and planning & supervision of day-to-

day activities of microbiology department.

. Responsible for R&D for dehydrated culture media and media bases.

. Responsible for microbiological testing of various dehydrated culture

media, bulk / food / agro products.

. Responsible for water testing, raw material testing, finished goods

testing, environmental monitoring, plant hygiene and documentation.

. Preparation of protocols and reports for media sterility. Preparation

of method of analysis for micro tests, Technical specifications, SOP,

MSDS, etc.

. Qualification check of equipments, e.g., Steam sterilizer, Dry heat

sterilizer, Incubators, UV-Vis spectrophotometer, etc.

. Coordinating with the production department and ensuring media fills.

. Isolation, identification and maintenance of microbial isolates.

. Facing Regulatory and Customer audits related to microbiology.

Awards/fellowships Received

* Received research fellowship during 08/07/2008 to 01/12/2011 from

University Grants Commission under the scheme UGC Research Fellowships

in Science for Meritorious Students (UGC-RFSMS).

* Qualified CSIR-UGC National Eligibility Test (June 2012) for

Lecturership.

List of Publications

1. Singh SK, Tripathi VR, Jain RK, Vikram S, Garg SK. An antibiotic, heavy

metal resistant and halotolerant Bacillus cereus SIU1 and its

thermoalkaline protease. Microbial Cell Factories 2010;9:59.

2. Singh SK, Singh SK, Tripathi VR, Khare SK, Garg SK. A novel

psychrotrophic, solvent tolerant Pseudomonas putida SKG-1 and solvent

stability of its psychro-thermoalkalistable protease. Process

Biochemistry 2011;46,1430-1435.

3. Singh SK, Singh SK, Tripathi VR, Garg SK. An Oxidant, Detergent and

Salt Stable Alkaline Protease from Bacillus cereus SIU1. African

Journal of Biotechnology 2011;10,122**-*****.

4. Singh SK, Singh SK, Tripathi VR, Khare SK, Garg SK. Comparative one-

factor-at-a-time, response surface (statistical) and bench-scale

bioreactor level optimization of thermoalkaline protease production

from a psychrotrophic Pseudomonas putida SKG-1 isolate. Microbial Cell

factories 2011;10:114.

5. Singh SK, Singh SK, Tripathi VR, Garg SK. Purification,

characterization and secondary structure elucidation of a detergent

stable halotolerant thermoalkaline protease of Bacillus cereus SIU1.

Process Biochemistry 2012;47(10):1479-1487.

6. Garg SK, Tripathi M, Kumar S, Singh SK, Singh SK. Microbial

dechlorination of chloroorganics and simultaneous decolorization of

pulp-paper mill effluent by Pseudomonas putida MTCC 10510 augmentation.

Environmental Monitoring and Assessment 2012;184(9):5533-5544.

7. Singh SK, Singh SK, Tripathi VR, Garg SK, Khare SK. Downstream

processing, characterization and structure-function relationship of

solvent-, detergent-, psychro-, thermo-, alkalistable metalloprotease

from metal-, solvent-tolerant psychrotrophic Pseudomonas putida SKG-1

isolate. Biotechnology Progress 2013;29(1):99-108.

8. Singh SK, Garg SK. Shake-flask and bench-scale stirred tank bioreactor

production optimization of a thermoalkaline protease from Bacillus

cereus SIU1 using one-factor-at-a-time and response surface

(statistical) methodologies. Biocatalysis and Biotransformation

2014;32(3):173-187.

9. Garg SK, Singh SK. Extreme environments: Goldmine of industrially

valuable alkali-stable proteases. Journal of Basic Microbiology

(Revision submitted).

Submitted DNA sequence(s) to NCBI

1. Submitted a 1456 bp 16S rDNA sequence of Bacillus cereus to Genbank

under accession number FJ 976896.

2. Submitted a 1488 bp 16S rDNA sequence of Pseudomonas putida to Genbank

under accession number HQ 259593.

Seminars/conferences/symposia

1. Singh, Sanjay K; Tripathi, V.R. and Garg, S.K. (2010). Studies on

thermoalkaline protease producing halotolerant bacteria from the soil

of eastern Uttar Pradesh. Poster presented in Conference on

"Bioprospecting: Access for Sustainable Development" held at Motilal

Nehru National Institute of Technology, Allahabad, U.P., India, during

19-20 February, 2010.

2. Singh, Sanjay K; Singh, Santosh K; Tripathi, V.R. and Garg, S.K.

(2011). Isolation and growth behavior of a halotolerant, antibiotic and

heavy metal resistant bacterial isolate from alkaline soil: An

indicator of environmental stress. Paper presented in Conference on

"Climate Change and its Impact on Biological Communities" held at Dr.

Ram Manohar Lohia Avadh University, Faizabad, U.P., India, during 12-13

February, 2011.

3. Singh, Sanjay K; Singh, Santosh K; Tripathi, V.R. and Garg, S.K.

(2011). A halotolerant, thermoalkaline protease of Bacillus cereus

SIU1: Production optimization, purification and characterization.

Poster presented in Conference on "Frontiers in Biological Sciences"

held at Veer Bahadur Singh Purvanchal University, Jaunpur, U.P., India,

during 4-5 December, 2011.

Skills Mastered

* Isolation/identification of bacteria and fungi

* Isolation of bacterial DNA/RNA, PCR

* Fermentation optimization at shake-flask and bench-scale bioreactor

level

* Protein separation and purification by chromatographic techniques

* Determination of protein secondary structure using CD

* Native PAGE, SDS- PAGE, Activity gel electrophoresis

* Analysis of effect of environmental factors on activity/structure of

enzymes

* Immunochemical techniques including agglutination, ouchterlony double

diffusion, immunnoelectrophoresis, etc.

* Routine microbiological, biochemical assays

Strengths

* A self-starter with the ability to deliver under pressure, and meet

deadlines effectively.

* Capable of working independently and collaboratively.

* Capable of conducting seminar & workshops.

* Have good organizational, interpersonal and analytical skills.

Additional Skills

* Good computer operation skills, including MS office and internet.

Personal Information

Father's name : Shri Udai Raj Singh

Date of Birth : November 18th, 1984

Marital Status : Single

Language known : English, Hindi

Declaration

I hereby declare that the above-mentioned information is correct up to

my knowledge and I bear the responsibility for the correctness of the above-

mentioned particulars.

Date:

Place:

(Sanjay Kumar Singh)

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