molecular biologist

H u imin Xie, Ph.D.

**** ****** ***** ***** *****:

x acgcn0@r.postjobfree.com

San Diego, CA 92130 Cell: 312-

*** ****

C itizenship: Permanent Resident (Green Card)

P rofile :

• I am an expert cell, molecular and i n vivo b iologist w ith over 10+ years of

hands-on experience in multiple fields, including i mmunology, neuroendocrinology and

epigenetics. H ighly motivated and ability to multitask, successful in establishing cell- and

q PCR-based assay development . E xcellent interpersonal and time management skills, team

p layer, outstanding writ ten and verbal communication.

P rofessional Experience & Education:

2009.11 present: Postdoctoral Fellow (Laboratory of Dr. Pamela L. Mellon )

Dept. of Reproductive Medicine, University of California at San Diego (La Jolla, CA)

T he overall focus of my current research is to investigate molecular and epigenetic

mechanisms involved in gonadotrope development, t hus elucidating the understanding of

reproduction and infertili ty.

• Di rected and managed separate projects to elucidate gene regulation and function of

several homeodomain t ranscription factors i n gonadotrope development using cell-based

f unctional assays, qPCR-based assays, i n vitro b inding assays, and i n vivo t ransgenic

m ice.

• Cha racterized epigenetic modulation d uring gonadotrope development, including

h istone modification and DNA methylation using ChIP-qPCR assays.

• Established b iotinylated BLRP-tagged system for genome-wide location analysis.

• Awarded a 2-year N I H Individual Postdoctoral grant in Neuroendocrinology on the first

submission.

• Mentored M aster student for individual research and disseration (UCSD BS/MS

p rogram).

• T rained u ndergraduate students for individual research (UCSD BISP 199 program).

2001.8 2006.11: P h. D in I mmunology (Laboratory of Dr. Zuoming Sun )

Dept. of Microbiology & Immunology, University of I l linois at Chicago (Chicago, I L)

T he focus of my thesis was to i nvestigate the signaling pathways/molecular mechanism

i nvolved in CD4+CD8+ double-positive (DP) t hymocyte survival and peripheral T cell repertoire .

• Developed and utilized m ulti-color f low cytometry and T cell sorting to measure T cell

development, apoptosis/survival and cell cycle phases.

Huimin Xie---Scientist

• Elucidated f unctions of (1) RORγ t, and (2) β-catenin/TCF-1 during thymocyte

development, both i n vitro and i n vivo.

• I ndependently identified t he function and link between β-catenin/ TCF-1 and RORγt

pathways in the regulation of thymocyte survival using microarray, cell-based assays and

i n vivo studies.

• Supported g roup members in multiple research projects, including activation-induced

cell death of T cells and t ranscriptional regulation of CD4 expression by β-catenin /TCF-1.

• T rained u ndergraduates as research assistants.

1998.9 2001.6: M .S. Degree in Biochemistry (Laboratory of Dr. Jin Zhang)

Key Lab of Molecular Enzymology and Engineering, Jilin University (Changchun,

China)

Thesis: Purification and Characterization of Hybrid L-Aspartase

1994.9 1998.6: B.S. in Biotechnology

College of Life Science, Jilin University (Changchun, China)

Thesis: I solation and Characterization of Anti-heat Bacterium from Changbai Mountain

Skills/Qualifications:

Molecular and Cellula r Biological Animal Manipulations:

Techniques: Rodent surgical procedures and

Cell culture & t ransfection behavior assessment

Reporter gene expression Tissue dissections & collections

In tracellular staining Primary cell culture

Electroporation Fertili ty assessment & hormone

measurement

Quanti tative real-t ime PCR (qRT-

PCR) assays H istological sectioning &

i mmunohistochemistry (IHC)

Molecular cloning techniques

Rodent animal dosing & drug

Generation of stable cell lines

administration

Western blot & Northern blot

Generation of t ransgenic mice &

siRNA knockdown

conditional knockout mice

Co-immunoprecipitation (Co-IP)

Colony management/breeding

Electrophoresis mobility shift assay

Phenotype screening & genotyping

(EMSA)

Communication:

Microarray

Grant and manuscript wri ting

Immunological Techniques:

Editing & reviewing scientific

Mul ti-color F low cytometry

journals

T cell isolation, sorting & culture

Huimin Xie---Scientist

Apoptosis & cell survival assays Preparation & execution of oral

p resentations

Cell cycle assay

Teaching and mentoring students

T cell activation & activation-induced

cell death Analytical Skills:

Recombinant adenovirus generation Statistical analysis softwares (JMP

& i nfection and GraphPad Prism)

Data & image editing software, such

Epigenetic Techniques:

as Photoshop, I l lustrator, Canvas,

Chromatin immunoprecipitation

E ndnote

(CHIP)

ApE p lasmid editor, DNA & protein

DNA methylation and sequencing

sequence analysis BLAST

DNase sensitivity assay

P ublications (selected):

1. Xie H, Meadows JD, Hoffmann HM, Mayo SL, C rystal T, Sunamita S. Leming, Chiara Maruggi,

Shannon W. Davis, Rachel Larder, and Mellon PL. Homeodomain proteins Six6 and Six3 regulate

gonadotrope-specific genes during gonadotrope development. M ol Endocrinol . I n review.

2. Xie H, I yer AK, Ngo C, Mellon PL. Epigenetic regulation of gene expression in developmental stages

gonadotrope cell lines. I n preparation .

3. Xie H, Cherrington BD, Meadows JD, Witham EA, Mellon PL. Msx1 Homeodomain Protein Represses

αGSU and GnRH Receptor Genes During Gonadotrope Development. M ol Endocrinol . 2013 Mar;

t he

27(3):422-36.

4. Wang R, X ie H, Huang Z, Shang W, Sun Z. Developing and activated T cell survival depends on

d ifferential signaling pathways to regulate anti-apoptotic Bcl-xL. C l in Dev I mmunol . 2012; 2012:632837.

5. Wang R, X ie H, Huang Z, Ma J, Fang X, Ding Y, Sun Z. T ranscription factor network regulating

CD4+CD8+ t hymocyte survival. C rit Rev I mmunol . 2011; 31(6):447-58.

6. Wang R, X ie H, Huang Z, Ma J, Fang X, Ding Y, Sun Z. T cell factor 1 regulates thymocyte survival via a

RORγ t-dependent pathway. J ourna l of I mmunology. 2011 Dec 1; 187(11):5964-73.

7. Huang Z, X ie H, Wang R, and Sun Z. Retinoid-related orphan receptor γt is a

potential therapeutic target for controlling in flammatory autoimmunity. E xpert

O pinion on Therapeutic Ta rgets. 2007, 11(6):737-743.

8. Huang Z, X ie H, Ioannidis V, Held W, Clevers H, Sadim MS, Sun Z. T ranscriptional

regulation of CD4 gene expression by T cell factor-1/beta-catenin pathway. J ourna l of

I mmunology. 2006 Apr 15; 176(8):4880-7.

9. Xie H, H uang Z, Wang R, Sun Z. Regulation of thymocyte survival by t ranscriptional

coactivators.

C r itical reviews in I mmunology. 2006, 26(6): 475-486

10. Xie H, H uang Z, Sadim MS, Sun Z. Stabilized beta-catenin extends thymocyte

survival by up-regulating Bcl-xL. J ourna l of I mmunology. 2005 Dec 15; 175(12):7981-

8.

Huimin Xie---Scientist

11. Xie H, Sadim MS, Sun Z. RORgamma recruits steroid receptor coactivators to ensure

t hymocyte survival. J ourna l of I mmunology. 2005 Sep 15; 175(6):3800-9.

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