Senior Staff Scientist
Resume:
RENAY BUCKERY
Wilmington, DE 19808
EXPERTISE SUMMARY:
Laboratory Skills:
* Assay development and validation (ELISA, ECL, ADA, SPA, filtration binding).
* Tissue culture (various cell lines: mammalian, insect, etc.)
* GLP practices
* Lab coordinator
* Clinical lab (phlebotomy, chemistry, RIA, hematology, toxicology and blood bank)
* ASCP certification
* Phlebotomy
* Molecular biology (cloning, transfections, DNA isolation, PCR)
* Western blots, SDS PAGE
* High through put screening
Equipment and Computer Skills:
*IMMULITE 100 autoanalyzer
*Detection systems: Molecular Device Spectramax Max L and Spectramax plus (Chemiluminescence and
Fluoresence), MSD Sector Imager 6000 (Electrochemiluminescence), Gamma Counter.
*Centrifuges, plate washers
*Microsoft Office – Excel, Word, PowerPoint
*Watson LIMS 7.4.1
*SoftMax Pro 3.1.2
*Other analyzers in hospital setting
SELECTED ACCOMPLISHMENTS:
Valuable contributor to bringing one of the first anti-HIV drugs (Sustiva (r))to market. Developed, validated
and exceuted the p24 antigen ELISA assay that provided the company with vital information.
PROFESSIONAL EXPERIENCE:
QUEST PHARMACEUTICAL SERVICES – Feb. 2008 to present
Senior Staff Scientist, Biomarker Department
* Develop and validate novel immunoassays, commercial assays and cell based assays to support various
drug discovery projects for Phase I through Phase III preclinical and clinical studies.
*Maintain various cell lines for cell based assays.
*Responsible maintaining the functionalities of a tissue culture lab.
* Optimize assays for sensitivity and selectivity.
* Validate assays for robustness and stability.
* Perform assays for, method development, validation and sample analysis
* Communicate with client, outside vendors and QPS’s quality assurance to efficiently meet timelines on
multiple projects simultaneously.
* Troubleshoot and investigate any observed problems in the study.
* Process data using Watson LIMS 7.4.1 and SoftMax Pro 3.1.2
* Evaluate significance data & report findings
* Perform client studies under FDA and GLP regulations
* Write and review analytical procedures, validation protocols and bioanalytical study plans
* Lab coordinator responsible for communicating with vendors,, negotiating pricing, researching products,
checking availability, POs, maintaining supplies in stockroom for the group.
QUEST PHARMACEUTICAL SERVICES – Feb. 2006 to Feb. 2008
Staff Scientist, Biomarker Department
*Manage biological preclinical and clinical samples, commercial and proprietary reagents and compounds.
*Maintain corresponding documents such as sample receipt, shipment information, storage documentation
according to GLP regulations.
*Perform assays for method development, validation and sample analysis according to client specifications
and under FDA and GLP regulations.
*Performed clinical studies using IMMULITE 1000 autoanalyzer
*Responsible for training analysts on IMMULITE 1000.
*Process data using Watson LIMS software.
*Evaluate significance of data and report findings.
*Manage, organize, stock lab supplies for the group to increase productivity and efficiency.
QUEST PHARMACEUTICAL SERVICES contract through Kelly Services – Oct. 2005 to Feb. 2006
Associate Scientist, Biomarker Department
*Develop and validate novel immunoassays and cell based assays.
*Evaluate data and report findings.
*Work under GLP regulations.
* Perform assays for sample analysis.
DUPONT PHARMACEUTICALS COMPANY
(formerly Dupont Merck Pharmaceuticals, now Bristol-Myers Squibb)
Staff Scientist, Leads Discovery
* Developed, validated, and executed high through put cell based and receptor binding assays.
* Conducted filtration, SPA, flashplate and luciferase assays for 7TM GPCR high throughput screens.
* Reformatted assays into 96 and 384 formats which increased through put and cut cost of the screen.
* Executed Activity Base for data storage, PRISM for data analysis and Microsoft office.
* Performed phlebotomy for various therapeutic groups which enabled them to reduce operating costs.
Associate Staff Scientist, Molecular Biology, HIV Protease
* Constructed library of site directed mutants of the HIV protease gene.
* Characterized drug resistant profile of Sustiva(r) and other new drug candidates by site directed mutant
viruses.
* Developed, validated, and executed the p24 antigen ELISA assay which measured HIV virus production as
required in the Inter-company consortium Consensus Assay.
Associate Staff Scientist, Gene Therapy, Research and Development Department, Virology Group
* Established and operated high throughput cell-based assay for the detection of novel new enhancing
reagents for receptor-mediated gene delivery.
* Produced high quality plasmid DNA for gene delivery experiments.
* Cultured HeLa & CaCo2 cells.
* Skilled with histology techniques, cryostat operations and various enzyme assays on tissues.
* Expressed and purified Wt (Val 82) and resistant (Ala 82) HIV proteases.
* Experienced with PCR.
Associate Staff Scientist, Viral Diseases
* Experienced with influenza B virus production using embryonated chicken eggs.
* Produced and isolated recombinant proteins in Baculovirus.
* Developed and executed ELISA, DNA sequencing gels, PCR.
Laboratory Technician, Medical Product and Central Research & Development Department, Viral Diseases
Group
* Cultured Hela and CaCO2 mammalian cell lines and SF9 insect culture systems.
* Implemented automated mechanism-based assays (HIV TAT).
* Experienced with cell-free assays systems.
* Developed and executed two high throughput screens: HIV TAT and Reverse Transcriptase.
* Assisted with mechanism-based assay to determine the modes of action of several antiviral compounds.
* Conducted assays safely with isotopes (125I, 3H, 14C, & 35S).
* Experienced with various biochemical separation techniques.
Laboratory Technician in clinical laboratories in various Philadelphia Hospitals
* Worked in all departments of clinical lab (chemistry, RIA, hematology, blood bank, toxicology, coagulation
and phlebotomy).
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