Executive Plant

CURRICULUM VITAE

Anil Sahebrao Dakle

*****-*********@*****.***

Contact: 917*********.

Career Objective:-

Seeking an appropriate position for working with an organization where my commitment,

skills and team-orientation will be of value and gives me a chance to take part role in

growth of company, to update my knowledge and enhance my skills.

PROFESSIONAL EXPERIENCE: - (Total Experience: - 3 Years 4 Months).

Currently Associated with USV Ltd. From 11st Aug 2014 to till date.

Designation : Executive – Biologics Operation Downstream department.

Working as Executive - Biologics in Biologics Operation in production in downstream

department at USV Ltd. Navi Mumbai.

Experience on Purification of Filgrastim (Granulocyte colony stimulating growth

factor), Pegfilgrastim from bacterial cells.

Working Experience in Syngene International Ltd. from 27th Jan 2014 to 08th Aug

2014.

Designation: Executive - Biologics in Production Downstream department

(Biopharmaceutical Development).

Working as Executive - Biologics in BPP (Biological pilot plant) in production in

downstream department at Syngene International Ltd. a Biocon Company at

Bangalore.

Experience on Purification Monoclonal Antibodies, Recombinant Proteins and

Enzymes from mammalian and bacterial cell culture.

Working Experience in Wockhardt Biotech Park Ltd. from 05th Sep 2011 to

17th Jan 2014. (2Years, 5 Months).

Designation : Senior Officer in DSP.

Worked as Senior Officer in production in downstream department at Wockhardt

Biotech Park Ltd., Aurangabad.

Experience on purification of r-Human Insulin & Insulin Glargine in downstream area.

Job Profile:-

To carry out the chromatographic operations like Affinity, Ion Exchange,

Reverse phase, Hydrophobic Interaction chromatography, Expanded Bed

chromatography.

To carry out Column Packing/Unpacking and its evaluation of different ranges

of column BPG 100/950, BPG 200/500, BPG 100/500, QS 300/550, and QS

100/500.

Operation preparative RP-HPLC & LPLC (Novasep System).

RP-HPLC Column Unpacking and Packing, and its evaluation.

To carry out Ultrafiltration/Diafiltration.

To carry out continuous centrifugation operation.

To carry out aseptic preparation and filtration of buffers.

To carry out cell lysis, clarification and refolding.

To carry out the Virus filtration.

To carry out aseptic pooling and dispensing of drug substances.

To carry out operations in specific sections as per SOP, GMP and Safety norms.

To ensure cleaning and maintenance of equipment’s and facilities to the highest

standards, meeting GMP and regulatory standards.

Instrument / Equipment Handled.

Efficiently Operate and Trouble Shooting of Instruments:-

K-Prime 40-I, 40-II, 40-III chromatography system.

AKTA Pilot and AKTA Process chromatography system.

Tangential flow filtration.

Lyophilizer (Quanta 30XB, 144XB).

Continuous Centrifuge (Alfa Laval, West Falia, Pennwalt).

Integrity Test Machine (Millipore Integritest4, Sartocheck3).

Analytical HPLC (Agilent Technology, Shimadzu Co.)

Effective drying of protein with the help of Vacuum Dryer.

Having knowledge of SCADA, PLC and DCS system.

Laminar Air Flow Unit (LAFU).

Micro fluidizer (Microfluidics) and Ultra Taurrax T50 (Homogenizer).

Deep Freezer, Cold cabinet.

Moisture Analyzer, PH meter, Conductivity meter (Metller Toledo).

Reactions related to Filgrastim:-

Solubilization, Refolding, EK Reaction and Pegylation Reaction.

Reactions related to Insulin:-

Conversion reaction:-

Charging, monitoring and harvesting of conversion reaction.

To suggest some improvement in process & equipment’s.

Hydrolysis reaction:-

Charging, monitoring and harvesting of hydrolysis reaction.

Insulin purification technique:-

Crystallization.

Precipitation.

Cell Lysis and Refolding.

• Technical Documentation:-

K

nowledge of documentation like BMR, SOP preparation and review of the same,

Log books.

H

andled quality system documents like Deviation, Change Control, deviation

CAPA.

C

oordination with Production, QC & QA.

• Production planning and control:-

Preparing SOP and implementing effective techniques to improve product quality.

Handling plant operation to ensure timely completion of production target within

planned time and cost parameter.

Educational Background:-

Sr. Year Of

No. Passing

Qualification Class/Grade College/Institute Name University/ Board

Dr. Babasaheb Ambedkar

Marathawada University,

1 M.Sc. (60.91) 2011 Government Institute Of Aurangabad

Biotechnology Science, Aurangabad

First Class

Dr. Babasaheb Ambedkar

Marathawada University,

2 B.Sc. (69.36) 2009 Shiv Chhatrapati Aurangabad

Biotechnology College, Aurangabad

First Class

3 H.S.C. (64.83) 2006 Deogiri College, Maharashtra State Board

Aurangabad.

First Class Aurangabad

4 S.S.C. (70.66) 2004 Rajarshi Shri Shahu Maharashtra State Board

Vidyalaya, Aurangabad.

First Class Aurangabad

Projects:-

Project done on “Isolation, Production, Purification of lipase from dairy soil

sample”.

Trainings:-

One month “Implant training in Carlsberg Ind. Pvt. Ltd, Aurangabad”

Computer Skills:-

Windows 98, Windows XP.

MS-OFFICE. (MS Word, MS Excel, MS Power Point).

Personal Profile:-

• : 06Th Oct 1988

Date of Birth

• Citizenship : Indian

• Gender : Male

• Marital Status : Single

• Languages Known : English, Hindi and Marathi.

• Mailing Address : At. Dongargaon (Kavad),

Post. Pimpalgaon (Gangdev)

Tal. Phulambri,

Dist. Aurangabad.

Maharashtra. Pin-431111.

• Contact No. : +917*********.

Reference:-

Mr. Nilesh B. Ankulwar, Executive, Syngene International Ltd. Bangalore.

Contact No. +919*********.

Mr. Anil Sahebrao Dakle

Contact this candidate