Manufacturing Associate with GMP, GLP, Cell Culture experience

THET PYONE

**** *********** ******, *******, ** * 4536

CELL: 510-***-**** ************@*****.***

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Professional Summary

As an u ndergraduate research assistant for 1 year under supervision of Dr. Yi Tang at

UCLA, I not only had a chance to get a handful of experience but not limited to growing cell-

culture, protein purification, gene knock -out, Enzyme assay, PCR and associating analytical

techniques. Needless-to-say, getting a chance to work at Boehringer Ingelheim as a manufacturing

analyst position helped me apply my lab-scale techniques to industrial level techniques. Working

in Biotech industry has always been my dream and I am hoping to broaden my knowledge not

only in manufacturing department but also the departments I am new to.

Skills

P rotein Purification

Automated DNA sequencing (Data analysis)

ELISA

Transformation (E.coli and fungal strains)

D ialysis

PCR Primer Design

S DS Page

HPLC (affinity, ion exchange, his-tag)

Knowledge of plasmid preparation, Ligation & Digestion Western blot

B acterial protein expression

DNA extraction

Cloning

DNA mini-prepping

M ammalian Cell Passaging

Microscopy Techniques

Kinetics Assay (Data Analysis)

Media & Buffer Preparations

P athogenic Strains

Good Laboratory Practices (GLP)

CIP/SIP

SDS Page (Data Analysis)

cGMP

Enzyme Digestion

GDP

Excellent recording data/protocol in notebook

Autoclaving Cell culture (industry level)

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Work History

Manufacturing Analyst, 08/19 to current

Boehringer Ingelheim (Contract)

Assembly of Bioreactors (30L scale up to 12K)

Performing procedures strictly from SOPs

Taking QC samples (rinse samples and swab samples)

CIP/SIP bioreactors/filter carts and lines

Analytical equipment knowledge not limited to Rapidlab Analyzer(pH, pCO2, pO2),

Bioprofile 400 analyzer (lactate, glucose, glutamine, glutamate, ammonium), CeDex Cell

Counter, Nucleocounter, Osmolality

Familiar with DeltaV software, QCLIMS, BIOMES(equipment use log)

Current good manufacturing practice

Good documentation practice

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Directed Undergraduate Researcher, 01/2014 to 06/2014

UCLA – Dr. Tang (Chemical Engineering)

Purification of Cre-recombinase Protein for Nano capsule drug delivery

Techniques: Transformation of bacteria, plasmid extraction, DNA mini -prepping,

inoculation, Protein

purification, Primer Design, preparation of LB broth, TB broth and relevant antibody

resistant Agar plates,

Bradford Assay, Western Blot Analysis, Ligation DNA gel analysis, column chromatography.

Knock-out genes responsible for Psychrophilin B protein in Aspergillus

Techniques: fungus transformation, DNA extraction from fungus, GMMT & SMMT

preparation, PCR, Southern

blot analysis, Hygromycin resistant strain, protoplasting, lyophilizing.

Math Tutor, 08/2009 to 05/2011

Ohlone College

Assist students in Algebra self-paced class based on individual needs and learning styles.

Explain mathematical problems in comparison to real life.

Peer Mentor, 03/2009 to 08/2010

Ohlone College

Responsibility: Organize Welcome Day Event, Answer phone calls from new students and

a nswer their questions in a professional manner. Attend weekly meetings and discuss

s trategies for fund -raising, negotiate with local business for fund-raising events and plan

v arieties of events for the entire year for freshmen.

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Education

Bachelor of Science, Biochemistry (Spring 2014)

University of California, Los Angeles - L os Angeles

Accomplishments

President’s Honor Roll – 2008, 2009

Raymond F. Quan HOSTS Scholarship – 2010

UCLA Scholarship Recognition Award – 2011

Relevant Coursework

G eneral Biology Laboratory Skills (Microscopy Techniques, Rat Dissection, Agarose Gel

E lectrophoresis)

G eneral C hemistry Laboratory (Titration and etc.,)

Organic Chemistry Laboratory (Organic reactions, polymer chemistry, etc.,)

Biochemistry Lab 153L

Project: Purification of Lactose Dehydrogenase from a beef stew from local butcher

Biochemistry Lab 154

Project 1: Investigation of Lac Repressor and Lac Operator Binding affinity using site-

directed mutagenesis and B eta -Galactosidase Assay

Project 2: Investigation of Proteasome by Affinity Chromatography and In-vivo Proteasome

Activity Assay

Project 3: Investigation of self-splicing RNA from bacteriophage Twort

C hemical T hermodynamics

Energy Metabolism & its regulations

DNA, RNA & Protein Synthesis-Knowledge of how transcription and translation work, Sanger

Method for DNA s equencing

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