JuYoun Beak (also known as JuYoun Baek), Ph.D.

*** ****** ****** ****, ****, NC 27519

919-***-**** (Cell phone), 919-***-**** (Home)

E-mail: acg2e7@r.postjobfree.com

EDUCATION

Ph.D, Microbiology, August 2003

Kyungpook National University, Daegu, South Korea

Department of Microbiology, College of Natural Sciences,

Research Advisor: YoungHo Kim

Proposed Thesis Title: Characterization of human phosphoserine aminotransferase and 3

-phosphoglycerate dehydrogenase (PHGDH) involved in the phosphorylated pathway of L-serine

biosynthesis

Master of Science, Microbiology, February 2001

Kyungpook National University, Daegu, South Korea

Department of Microbiology, College of Natural Sciences

Bachelor of Science, Microbiology, February 1996

Kyungpook National University, Daegu, South Korea

Department of Microbiology, College of Natural Sciences

TEACHING & TUTORING EXPERIENCE

Mentoring, NIEHS, 2 graduate students

• Danielle Ackerman between 05/06-05/07

• Nana 05/04-05/06

Teaching Assistant, University of Kyungpook National University

Immunobiology Laboratory, 3 quarters between 3/01-8/03

• Assisted in the lab instruction of junior and senior level microbiology students

• Instructed students to design new experiments and learn proper safety practices

• Supervised advanced lab methods: microscopy, FACS(Fluorescence-activated cell sorting)

machine.

Teaching Assistant, Pohang Colleage

• Immunobiology and Signaling transduction, 2 quarters between 1/02-1/03

• Helped undergraduate students understand immunology concepts through face to face

appointments

Teaching Assistant, University of Kyungpook National University

Microbiology, spring semester 2002

• Assisted in instruction of general microbiology lab sections and a discussion section.

• Course emphasis in Microscope techniques.

RESEARCH EXPERIENCE

Associate Postdoc, Pharmacy department, UNC Eshelman School of Pharmacy, Chapel Hill, NC

March ’12-December ‘13

• Characterize the family protein (ADP ribosylation factor 1 and 6) and the partner protein

ARFGAP protein related to the mechanism of small molecule inhibitor (drug-like molecule) in

vivo/in vitro system

• Characterize the small molecule test for toxicological studies

• Transfection siRNA or shRNA into the target cells and isolation of mRNA and then

confirmation of mRNA expression pattern with real time PCR.

• Isolation the ARF family protein (ADP ribosylation factor 1 and 6) and the partner protein

ARFGAP protein and investigation the related mechanism small molecule inhibitor (drug-like

molecule) and ARF family protein

• Phospholipid[PtdIns(4,5)P2] Delivery into the cell and measurement the downstream

signaling events

• Performed procedures and calculations crucial to quality controls, including high

performance liquid chromatography (HPLC), pH testing, instrument calibration, centrifugation and

volumetric/coulometric titrations.

• Earned a reputation for preparing accurate lab reports, sample logs, plant documents.

• Adept in detecting, diagnosing and contributing to the resolution of flaws and impediments

in the production of chemical products.

• Consistently adhere to Good Laboratory Practice (GLP) as well as safety protocols,

producing results that are accurate, safe and compliant with regulatory standards.

Associate Postdoc, Cell and developmental biology, UNC School of Medicine, Chapel Hill, NC

September ‘08-June ‘11

• Isolation mRNA from siRNA/shRNA transfected cells or mouse embryonic fibroblast cells

which isolated from knockout mice and investigation target gene using real time PCR or

regular PCR

• Investigation on the function of PITP (phosphatidylinositol transfer protein) in cell migration

and in architecture of focal adhesion

• Characterization of the function of PITP on growth factor signaling such as epidermal growth

factor (EGF), insulin, and platelet-derived growth factor (PDGF) in mammalian cells with

investigating target gene expression(mRNA expression level) and protein level.

• Earned a reputation for preparing accurate lab reports, sample logs, plant documents and

I did data analysis and conformity.

• Consistently adhere to Good Laboratory Practice (GLP) as well as safety protocols,

producing results that are accurate, safe and compliant with regulatory standards.

• I was trained several microscopies by professional expert.

Visiting Fellow, Cell Biology Group, Laboratory of Respiratory Biology, NIEHS, NIH, RTP, NC

October ‘03-August ‘08

• Characterize the expressed GLIS2 and GLIS3 proteins

• Characterize the GLIS2 and GLIS3 protein toxicity test used transfected cell lines

• Identification the function of GLIS3 (Gli-similar kruppel zinc finger transcriptional factor) in

osteoblast differentiation

• Transfection exogenous GLIS3 gene into the cells with selection marker and then selected

the clone which overexpressed GLIS3 and then isolation of mRNA from several cell lines.

• Microarray after isolation of mRNA from specific cells or tissue

• Southern blot using genomic DNA to find out mutant mice and northern blot using mRNA

and specific target gene probe.

• Characterization of the function of GLIS3 in polycystic kidney disease and β -cell

development in pancreas development

• Isolation DNA/RNA from mouse kidney and pancreas tissue during developmental

process

• Isolation of human and mouse GLIS3 proteins after cloning into several expression vector

such as virus and bacteria.

• Characterization what domain is important to function of GLIS3 in the cellular function and

related disease using the isolated GLIS3 protein.

• Determination of target sequence of GLIS3, transcription factor, with over 100 times of

EMSA (Electrophoretic mobility shift assay) and regular PCR.

• Consistently adhere to Good Laboratory Practice (GLP) as well as safety protocols,

producing results that are accurate, safe and compliant with regulatory standards.

Graduate Research Assistant, University of Kyungpook National University, 03/01-08/03

• Cloning, PCR, Protein isolation, generation of antibody for specific gene

• Identified and characterized the enzymes involved in the L-serine synthesis

• Developed methods to measure enzyme activity

PEER-REVIEWED PUBLICATIONS

Kang, H. S., Kim, Y. S., ZeRuth, G., Beak, J. Y., Gerrish K., Kilic G, Sosa-pineda, B., Jensen, J.,

Pierreux, C. E., Lemaigre, F. P., Foley, J. and Jetten, A. M. (2009) Transcription factor Glis3, a novel

critical player in the regulation of pancreatic-cell development and insulin gene expression. Mol.

Cell. Biol. 29, 6366 - 6379

Kang, H. S.*, Beak, J. Y.*, Kim, Y. S., Herbert, R., and Jetten, A. M. (2009) Glis3 is associated with

primary cilia and TAZ and implicated in polycystic kidney disease. Mol. Cell. Biol. 29, 2556 – 2569

(*; equal contribution).

Beak, J. Y., Kang, H. S., Kim, Y. S., and Jetten, A. M. (2008) Functional analysis of the zinc fingers

and activation domains of Glis3 and mutant Glis3 (NDH1). Nucleic Acids Res. 36, 1690-1702

Jun DY, Park HS, Lee JY, Baek, J. Y., Park HK, Fukui K, Kim YH. (2008) Positive regulation of

promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by

transcription factors Sp1 and NF-Y. Gene 414, 106-114.

Kim, Y. S., Kang, H. S., Herbert, R., Beak, J. Y., Collins, J. B., Grissom, S. F., and Jetten, A. M.

(2008) Krüppel-like zinc finger protein Glis2 is essential for the maintenance of normal renal

functions. Mol. Cell. Biol. 28, 2358-2363.

Kang, H. S., Angers, M., Beak, J. Y., Wu, X., Gimble, J. M., Wada, T., Xie, W., Collins, J. B.,

Grissom, S. F., and Jetten, A. M. (2007) Gene expression profiling reveals a regulatory role for

ROR and ROR in Phase I and Phase II metabolism. Physiol. Genomics. 31, 281-294. (*; equal

contribution)

Beak, J. Y., Kang, H. S., Kim, Y. S., and Jetten, A. M. (2007) Krüppel-like zinc finger protein Glis3

promotes osteoblast differentiation by regulating FGF18 expression. J. Bone Miner. Res. 22, 1234-

1244.

Kang, H. S., Beak, J. Y., Kim, Y. S., Petrovich, R. M., Collins, J. B., Grissom, S. F., and Jetten, A. M.

(2006) NABP1, a novel ROR -regulated gene encoding a single-stranded nucleic acid binding

protein. Biochem. J. 397, 89-99.

Baek, J. Y, Jun DY, Taub D, Kim YH., (2003) Characterization of human phosphoserine

aminotransferase involved in the phosphorylated pathway of L-serine biosynthesis. Biochem. J.

373, 191-200.

Baek, J. Y, Jun DY, Taub D, Kim YH., (2000) Assignment of human 3-phosphoglycerate

dehydrogenase (PHGDH) to human chromosome band 1p12 by fluorescence in situ hybridization.

Cytogenet Cell Genet, 89, 6-7

ARTICLES PREPARING

Beak, J. Y, Zhang, Q. (2014)

QS11, small molecule, regulates the golgi structure and receptor trafficking. Preparing

Beak, J. Y, Kang, H. S., Kaiser, K. L., Allen, E, Samson T., and Jetten, A. M. (2014)

GLIS3 and NF2: Regulation Membrane-Focal adhesion Dynamics and Migration. Preparing

Beak, J. Y, Kang, H. S., Samson T., and Jetten, A. M. (2014)

GLI1 modulates Rho family GTPases and enhances glioblastoma cell migration. Preparing

POSTER PRESENTATION AT SCIENTIFIC MEETINGS

1. Kang, H. S., Okamoto, K., Beak, J. Y., and Jetten, A. M. Retinoid-related orphan receptor

alpha (RORα ) positively regulates obesity, hepatic steatosis, and inflammation. 2010

Keystone Symposia, Nuclear Receptor: Development, Physiology and Disease. Keystone

Resort, Keystone, Colorado, USA. March 23.

2. Kang, H. S., Beak, J. Y., Angers, M., Wada, T., Xie, W., and Jetten, A. M. Physiological

functions of retinoid-related orphan receptor alpha (RORα ) in adipose tissue. 2008

Keystone Symposia, Nuclear receptors: Orphan brothers, April 01.

3. Kang, H. S., Angers, M., Beak, J. Y., Yang, X–P., and Jetten, A. M. Novel role of nuclear

receptors RORα and RORγ in the regulation of phase I and Phase II metabolic

pathways. 2007 Keystone Symposia, Nuclear receptors and Metabolism, March 29.

4 Beak, J. Y., Kang, H. S., Allen E, Kim, Y. S. and Jetten, A. M. The function of Gli1 involved

in the glioma cell migration and Rho signaling 2007 NIEHS Science Award Day.

5. Beak, J. Y., Kang, H. S., Allen E, Kim, Y. S. and Jetten, A. M. GLIS3, a novel function in

Uterus development and cell migration. 2006 NIEHS Science Award Day.

Kang, H. S., Beak, J. Y., Kim, Y. S. and Jetten, A. M. NABP1, a novel ROR γ -regulated

6.

gene encoding a single-stranded nucleic acid binding protein. 2005 NIEHS Science

Award Day.

7. Kim, Y. S., Kang, H. S., Beak, J. Y., Herbert, R., Jetten, A. M. Critical roles for Glis

Kruppel-like zinc finger proteins in developmental disease. 2005 NIEHS Science Award

Day

8. Kang, H. S., Angers, M., Beak, J. Y., and Jetten, A. M. Identification of Genes regulated

by ROR gamma in thymus. 2004 NIEHS Science Awards Day.

AWARDS & AFFILIATIONS

• Graduate Student Scholar Award, Kyungpook National University, 08/02 & 01/03

• Undergraduate Scholar Award, Kyungpook National University, 03/93-03/96

• Member, The Korean Society for Microbiology and Biotechnology, 03/02-08/03

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