Supervisor Project

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Takrima Haque

**** ********* ***, ********, **, J4W 3B9•514-***-****•*******.*****@****.******.**

Takrima Haque

Profile

A well-organized and dynamic researcher with post graduate education and

experience in cancer research, molecular biology, bioanalytical techniques,

proteomics, in vitro assays, etc. Extensive experience in interpretation and data

analysis, literature review, manuscript preparations, instrument troubleshooting.

Proficient in MS Word, MS Excel, MS Power point, Adobe photoshop. Superior

interpersonal, analytical and communication skills.

Highlights

Working on a certificate for clinical research/clinical trial monitoring

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Microbiological techniques

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Expert in mammalian cell culture techniques

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Western blotting

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Molecular biology

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Primer designing

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Expert in Immunofluorescence techniques

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Data presentation

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Fluent in English and French

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Experience

February 2013-May 2014

IRIC, Université de Montreal Montreal, QC

Research Associate

Worked in a project dealing with cell cycle to have a better understanding on targeting

cancerous cells. (Supervisors: Dr. Damien D’Amours and Dr. Vincent Archambault)

• Molecular cloning: inserting and mutating genes in vectors.

• Overexpressing/silencing MastL in Hela and U2OS cells (stable and transient

transfections). Stables were done by lentiviral production and transduction.

• FACS, immunofluorescence techniques.

• Manuscript writing, data collection and presentation.

February 2004 – Fall 2012

Montreal Children’s Hospital Research Institute Montreal, QC

PhD Student

Specialized in oncology - Worked on identifying potential therapeutic targets that were

specific to high grade pediatric astrocytomas. (Supervisor: Dr. Nada Jabado)

• Microarray analysis of pediatric astrocytoma samples: RNA, DNA, and protein

extraction from samples; sectioning frozen and FFPE slides; extracting cells using laser

capture micro-dissection; microarray slide perparation, hybridization and analysis

using genespring and ingenuity; RT-PCR and QRT-PCR.

• Focused on one gene over-expressed in pediatric glioblastoma: SNX3. Cell culture

work including over-expressing/silencing SNX3 in different glioblastoma cell lines

(stable and transient transfections). Effects of over-expressing and silencing of SNX3

were studied by several techniques, including: western blots; immunofluorescence

514-***-****•*******.*****@****.******.**

Takrima Haque

techniques; immunohistochemistry; cell proliferation assays; soft agar assays; FACS.

August 2002-October 2003

University of Alberta Edmonton, Alberta

Research Technologist

Worked on the effects of specific drugs used to treat osteoporosis in Rats. (Supervisor: Dr.

Hasan Uludag)

• Cell culture, DNA and protein quantification assays using Fluorescence spectrometry,

HPLC.

• Instrument trouble shooting, analysis and interpretation of data and ability to review

scientific literature.

• Book keeping, inventory and ordering of supplies in the lab.

June 1999-August 2001

McGill Montreal, QC

M.Sc Student

Thesis Title: Charting the unfolding of ATCase by isotope editing FTIR spectroscopy in

conjunction with 2D correlation spectroscopy. (Supervisor Dr. Ashraf Ismail)

• Isolation, expression and purification of protein from bacterial cells by ion exchange

chromatography

• Analysis of protein secondary structure by Fourier Transform Infrared spectroscopy and

Circular Dichroism

Education

Fall 2004-Fall 2012

McGill Montreal, QC

PhD Experimental Medicine

Summer 1999-Fall 2001 McGill Montreal,

QC

M.Sc. Food Science and Agricultural Chemistry

Fall 1996-Summer 1999 McGill Montreal, QC

B.Sc. Microbiology and Immunology

Publications

• Haque T, Faury D, Albrecht S, Lopez-Aguilar E, Hauser P, Garami M, Hanzély Z, Bognár L, Del

Maestro RF, Atkinson J, Nantel A, Jabado N. Gene expression profiling from formalin-fixed

paraffin-embedded tumors of pediatric glioblastoma. Clin Cancer Res. 2007 Nov

1;13(21):6284-92.

• Faury D, Nantel A, Dunn SE, Guiot MC, Haque T, Hauser P, Garami M, Bognár L, Hanzély Z,

Liberski PP, Lopez-Aguilar E, Valera ET, Tone LG, Carret AS, Del Maestro RF, Gleave M, Montes

JL, Pietsch T, Albrecht S, Jabado N. Molecular profiling identifies prognostic subgroups

of pediatric glioblastoma and shows increased YB-1 expression in tumors. J Clin

514-***-****•*******.*****@****.******.**

Takrima Haque

Oncol. 2007 Apr 1;25(10):1196-208.

• Haque T, Uludag H, Zernicke RF, Winn SR, Sebald W. Bone marrow cells from normal and

ovariectomized rats respond differently to basic fibroblast growth factor and bone

morphogenetic protein 2 treatment in vitro. Tissue Eng. 2005 Mar-Apr;11(3-4):634-44

• Varkey M, Kucharski C, Haque T, Sebald W, Uludag H. In vitro osteogenic response of rat

bone marrow cells to bFGF and BMP-2 treatments. Clin Orthop Relat Res. 2006

Feb;443:113-23

References

References are available on request.

Contact this candidate