Technician Manufacturing

Harbans Minhas

***** ****** *** #* ***** City, CA. 94587

510-***-**** ***********@*****.***

Manufacturing Lab Technician

Qualifications

Manufacturing Lab Technician DNA Sequencing

cGMP, SOP, OSHA, QA/QC Validation

Clean Room Manufacturing Cell Cultures

Professional Experience

Lab Technician 8/06-7/07

Genentech Inc. South San Francisco, Ca.

Operate and monitor Abec bioreactors using Allen and Bradley Skid adhering cGMP.

Responsible for the aseptic processing of components and products using adhering cGMP

by using the aseptic techniques, prepared the growth media and buffers to be used in cell culture.

Perform CIP, SIP, and pressure test on Abec bioreactor.

Responsible for the operation and preparation of manufacturing equipment for scale up used for

cell culture process, which includes bioreactor and recovery processes.

Generate and write Batch Production Records (BPR), Standard Operating Procedures (SOP).

Support and assist manufacturing, process development, QA/QC, validation.

Perform cell counts using Vi-cell and also perform the expansion of cell culture when required.

Responsible sterile preparation of components; aseptic processing filtering and filling of

pharmaceutical products; environmental monitoring of manufacturing facility.

Manufacturing Associate 1/05-11/05

Predicant Biosciences, South San Francisco, CA

Manufacturing process optimization to improve micro fluidics chips for proteomic biomarker

discovery.

responsible for collaborations with manufacturing group to help design manufacturing SOP

Performed meticulous sample preparation for protein biomarkers processes.

Prepare standard stock solutions using dilutions methods.

Worked with various polyethylene material molding and laminating machinery under a clean room

environment.

Applied analytical skills to troubleshoot material processing and machinery issues.

Promoted a safe work environment & provided recommendations on maintaining the safety of the

work environment.

Lab Technician 3/02-1/04

DNA, Sciences Fremont, CA

Development of molecular diagnostic kits for LQT syndrome Diagnostics.

QC Primers with use of spectrophotometeric method to determine the concentration of the primer

Perform production PCR using 384 well plates & Robotic dispensing (BiomekFx & Platemate).

Cycle Sequencing & Millipore cleanup using multiscreen 384 well plates & biomek Fx

DNA sequencing using Megabace 1000 & ABI PRISM 3700 DNA Analysis System.

Performing gel electrophoresis and run various DNA samples on agarose Gels for QC.

Applied analytical skills to troubleshooting Magabace 1000.

Independently handling lab plastic and regents ordering. Maintain the lab stock by using oracle

database.

Production Associate 9/99-10/01

Incyte Genomics, Fremont, CA.

Maintain Micro array printing equipment in clean room.

Windows based operator interface to control micro array printing equipment and visual tools

including video cameras and microscopes to verify printing quality.

Troubleshoot Micro array printing equipment, printing problems, robot alignment.

Service micro-scale printing devices. Hydrate 96-well biological plates with pipettes and automated

devices.

Perform glass slide washing procedures with semi-automated devices

Use protocols to ensure prevention of product contamination and identify, count, and report defects

in product.

Hybridization of fluorescent cDNA probes to cDNA micro arrays

Generate cDNA probes from mRNA, using RT and EtOH precipitation.

PCR, running PCR pilots at varying concentration of polymerase and different Concentration of

constituents of master mix cocktail and varying number of cycles. Sephacryl and multiscreen

purification of PCR product, Pico green, Agarose Gel electrophoresis, making Sephacryl columns,

Hyb Application support and make solution of different strength.

Education

BS in Biology & Chemistry, Punjab University, India,

Phlebotomist Technician, Gurnick Academy,Sanmeteo,CA

Contact this candidate