Development College
Resume:
CURRICULUM VITAE
Phillip J. Sanchez, Ph.D
************@*****.***
PERSONAL:
Home Address: * *********
Aliso Viejo, CA
92656
Citizenship: United States of America
WORK EXPERIENCE:
9/2013-06/2014
Temp. Research Scientist I High-throughput screening of small molecules
Infectious Diseases for antibacterial activity
Vertex Pharmaceuticals Determination of minimum inhibitory
Coralville, IA 52241 concentration, mutant prevention concentration,
and resistance frequency in bacterial strains
Assessment of antibiotic synergy/antagonism
8/2011 – 9/2013
Assistant Research Scientist Antibody development/characterization
Development of ‘Macrochip’ for screening
Laboratory of David Soll
Developmental Studies Hybridoma Bank antibodies against various cancer cell lines
Department of Biology Culture/Phenotype cancer stem cells
University of Iowa Identify novel cell surface molecules specific
Iowa City, IA 52242 to cancer stem cells and/or highly
metastatic/tumorigenic cells
8/2004 – 7/2011
Postdoctoral Fellow
Laboratory of Ross Kedl Flow cytometric analysis of dendritic cell
Integrated Department of Immunology (DC) subsets following immunization or
University of Colorado activation of Toll-Like receptors
National Jewish Health Identify cell surface molecules involved DC-T
Denver, CO 80206 cell interactions
Identify mechanisms of vaccine-induced
development of T cell activation and memory
6/2003 – 8/2004
Postdoctoral Fellow Determine the effect of alcohol consumption
Laboratory of Gary G. Meadows on natural killer cell development by studying
Cancer Prevention and Research Center the distribution of NK cell progenitors
College of Pharmacy Determine the effect of alcohol consumption
Washington State University on NK cell proliferation by studying IL-2
Pullman, WA 99165 receptor signaling and the expression of cell
cycle proteins
EDUCATION:
2003 Ph.D., Pharmacology/Toxicology
Department of Pharmaceutical Sciences
College of Pharmacy
Washington State University
Pullman, WA 99165
Thesis Advisor: Gary G. Meadows
Dorothy O. Kennedy Distinguished Professor
Director, Cancer Prevention and Research Center
Dissertation Title: The Effect of Ethanol Consumption in C57BL/6 Mice on Natural
Killer Cell Proliferation and Development
1995 Bachelor of Science, Biology
College of Science
California State Polytechnic University
Pomona, CA 91768
PUBLICATIONS:
Phillip J. Sanchez, Karla Daniels, and David R. Soll. Generating a Battery of Monoclonal Antibodies
Against Native Green Fluorescent Protein (GFP) for Immunostaining, FACS, IP and ChIP, Using a
Unique Adjuvant. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 33:80-88, 2014.
Phillip J. Sanchez and Ross M. Kedl. An alternative signal 3: CD8+ T cell memory independent of IL-12
and type I IFN is dependent on CD27/OX40 signaling. Vaccine, 30:1154– 1161, 2012.
Jonathan S. Kurche, Matthew A. Burchill, Phillip J. Sanchez, Catherine Haluszczak, and Ross M. Kedl
Comparison of OX40 Ligand and CD70 in the Promotion of CD4+ T Cell Responses. Journal of
Immunology, 185:2106 -2115, 2010.
McWilliams JA, Sanchez PJ, Haluszczak C, Gapin L, Kedl RM. Multiple innate signaling pathways
cooperate with CD40 to induce potent, CD70-dependent cellular immunity. Vaccine, 28:1468-76, 2010.
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Phillip J. Sanchez, Catherine Haluszczak, Hideo Yagita, and Ross M. Kedl. Combined-TLR/CD40
Stimulation Mediates Potent Cellular Immunity by Regulating Dendritic Cell Expression of CD70 In
Vivo. Journal of Immunology, 178:1564-1572, 2007.
Phillip J. Sanchez, Catherine Haluszczak, and Ross M. Kedl. Coordinated integration of Toll-Like
Receptor and TNF-ligand pathways mediates potent adaptive immunity. Research Signpost: Towards an
Understanding of Initiating T cell Immunity, Chapter 4 ISBN: 81-308-0118-3, 71-97, 2006.
Fu, YM, Yu, ZX, Li, YQ, Ge, X, Sanchez, PJ, Fu, X, and Meadows, GG. Specific amino acid
dependency regulates invasiveness and viability of androgen-independent prostate cancer cells. Nutrition
and Cancer, 45:60-73, 2003.
Daubert, DL, Meadows, GG, Wang, JH, Sanchez, PJ, and Speth, RC. Changes in angiotensin II
receptors in dopamine-rich regions of the mouse brain with age and ethanol consumption. Brain
Research, 816:8-16, 1999.
PATENTS:
Co-Author of United States Patent US8361471 B2
“Immunostimulatory Regimen Comprising Administering Type I Interferon and Agonistic Anti-CD40
Antibody”
University of Colorado
Co-Author of United States Patent Application US2007/11/681092.
Combined TLR/CD40-agonist DNA Vaccine Constructs.
University of Colorado.
INVITED SEMINARS:
Focus Group on Visible Ethnic Minority Scientist Participation 7/08
Keystone, CO
Intracellular and Intercellular Signaling in Dendritic Cell Function Meeting 2/07
Keystone, CO
Research Society on Alcoholism 6/04
Vancouver, BC, Canada
POSTER PRESENTATIONS:
IL-12- and Type I IFN-Independent Generation of CD8+ T Cell Memory 10/10
Keystone Symposia- Immunological Mechanisms of Vaccination Meeting
Seattle, Washington
TNFR-TNFL Superfamily Member Interactions Induced by Combined TLR/CD40 5/09
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Stimulation Act as a Signal 3 for Enhanced CD8+ T Cell Immunity
American Association of Immunologists Annual Meeting
Seattle, WA
The Role of TNF SuperFamily and Signal 3 Cytokines in CD8+ T Cell Memory 1/09
T Cell Memory Meeting
Keystone, CO
The Expression of CD70 on Dendritic Cells in vivo and its Role in Primary and 2/07
Memory CD8+ T Cell Responses
Intracellular and Intercellular Signaling in Dendritic Cell Function Meeting
Keystone, CO
Regulation of Dendritic Cell CD70 Expression in vivo and the role of CD27-CD70 9/06
Signaling in Synergistic Toll-Like Receptor/CD40 Agonist-Mediated Expansion of
CD8+ T Cells
Colorado Immunology Conference
Synergistic Toll-Like Receptor/CD40 Agonist-Mediated Expansion of CD8+ T Cells 9/05
is Regulated Through CD70 Signaling
Colorado Immunology Conference
HONORS AND AWARDS:
10/10 Travel Scholarship
Keystone Symposia- Immunological Mechanisms of Vaccination Meeting
Seattle, Washington
2/09 Travel Scholarship
Keystone Symposia Meeting- Immunologic Memory and Host Defense
Keystone, CO
2/07 Travel Scholarship
Keystone Symposia Meeting- Intracellular and Intercellular Signaling
in Dendritic Cell Function
Keystone, CO
9/06 First Place Postdoctoral Poster
7th Annual Colorado Immunology Conference
Breckenridge, CO
8/04-8/06 Post-Doctoral Immunology Training Grant
1996, Pre-Doctoral Training Grant, NIAAA
1999-2000
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LABORATORY TECHNIQUES:
Various microbiological techniques
Hybridoma production (fusion/cell culture/cloning)
Antibody isolation/characterization/conjugation
Flow cytometry (surface and intracellular)
Fluorescent microscopy/Immunohistochemistry
Protein electrophoresis (1-D & 2-D) and Western blot
Cancer stem cell culture
Isolation and enrichment of rare cell subsets (dendritic cells, natural killer cells, antigen-specific T cells,
cancer stem cells) by FACS and MACS
ELISA/ELISPOT
Infectious disease models (Listeria monocytogenes, Vaccinia virus)
Tumor models (human breast, prostate, and brain and mouse B16 melanoma)
Immunizations via multiple routes (ip, iv, sc)
Organ harvest (spleen, lymph nodes, blood, liver, lungs, ovaries)
Generation of bone marrow-derived dendritic cells
Culture and in vitro activation of primary and bone marrow-derived DCs
Ex vivo detection of primary and memory antigen-specific CD8+ T cell responses (tetramer)
Ex vivo peptide stimulation of antigen-specific CD8+ T cells (cytokine production)
Cell proliferation (CFSE, BrDU)
Cell-mediated cytotoxictiy (in vivo- differential CFSE labeling, in vitro- 51Cr release)
Automated liquid handling (Multi-Drop, BioMek)
DNA/RNA isolation
PCR
Software (Microsoft Office, graphing, FACS analysis (FlowJo/CellQuest/Summit))
TEACHING RESPONSIBILITIES:
Basic Biology of Human Disease Spring 2013
Metastasis, Tumorigenesis, & Motility of Cancer: Development of Models for New Therapies
Graduate Immunology Fall 2002
Fundamentals of Oncology/Tumor Immunology
Doctor of Pharmacy Immunology Fall 1998
HIV Vaccine Development
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REFERENCES:
1. Ute Müh, PhD
Associate Director, Infectious Diseases
Vertex Pharmaceuticals
2500 Crosspark Rd
Coralville, IA 52241
*******@****.***
2. Karla Daniels, PhD (Direct Supervisor)
Soll Lab
Senior Scientist
Department of Biology
College of Liberal Arts & Sciences
University of Iowa
301 BBE
Iowa City, IA 52245
*****-*******@*****.***
3. Ross M Kedl, PhD
Associate Professor
Integrated Department of Immunology
University of Colorado Denver
National Jewish Health
1400 Jackson Street
Denver, CO 80206
****.****@********.***
4. Gary G. Meadows, PhD
Associate Dean, Graduate Education & Scholarship
Dorothy Otto Kennedy Distinguished Professor, Pharmaceutical Sciences
College of Pharmacy
Washington State University
PO Box 646510
Pullman, WA 99164
*******@***.***
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