Assistant Vice President

Disha Panchani

**** ** ** ****** **** Apt # ***, Temple, TX 76504 267-***-**** ********.*****@*****.***

Education

Bachelors of Science May 2014 The pennsylvania state university

Major: Biotechnology

Minor: Microbiology

Skills & Abilities

Willing to relocate without any assistance

Strict attention to detail, excellent communication skills (written and oral), strong interpersonal skills, self-motivated and

responsible with strong work ethic, good time/priority management skills, ability to work both independently and in team

environment.

GLPs, Knowledge of FDA, cGMP and SOPs, proper use of laminar flow hood, BIOFLO 310 and RAMbio fermenters, and other

lab equipment.

Microbiology

Aseptic and sterile technique, bacterial staining, plating methods (streak, spread, pour), plate counting, enumeration and

identification of bacteria, use of biological safety cabinets, media and buffer preparation, food sample analysis, use of selective

media.

Biochemistry and Cell & Molecular Biology

DNA extraction, plasmid DNA preparation, restriction enzyme digests, PCR, agarose gel electrophoresis, SDS-PAGE, cell

fractionation by differential centrifugation, preparation of cells for cryopreservation, dissection chicken embroy, use of

hemocytometer

Chemistry

Solution preparation, titration, extractions, filtrations, separations, simple and fractional distillation, solubility tests, reflux,

recrystallization, chromatography

Experience

FINISH TECH CORPORATION MAY 2012 –

AUGUST 2012

CHEMISTRY LABORATORY ASSISTANT’S ASSISTANT

• PREPARED SAMPLES TO TEST FOR FURTHER PRODUCTION OF BATCHES

• MAINTAIN RECORDS OF ALL THE LAB TESTS PERFORMED, GIVE A UNIQUE IDENTITY CODE TO ALL THE

EXPERIMENTS

• VERIFY THE EXPERIMENT RECORDS FOR ACCURACY AND AUTHENTICITY

• Responsible for maintaining the laboratory, chemicals, apparatus and equipment effectively and in ready-to-use manner

• Maintain high standards of cleanliness and hygiene within the laboratory

PSU GENERAL AND APPLIED MICROBIOLOGY LAB RESEARCH AUGUST 2013 –

DECEMBER 2013

• AS A TEAM, WE ISOLATED AND IDENTIFIED A LACTIC ACID PRODUCING BACTERIA, LACTOCOCCUS LACTIS

FROM DIFFERENT SOFT CHEESE SAMPLE

• PREPARED MEDIA FROM SCRATCH TO ISOLATE LACTOCOCCUS LACTIS COLONIES (M17 PLATES AND MRS

BROTH)

• DETERMINED GRAM MORPHOLOGY BY GRAM STAINING SLIDES AND VIEWING UNDER MICROSCOPE

• PERFORMED LACTIC ACID ENZYME ASSAY TO DETERMINE LACTIC ACID PRODUCED BY BACTERIA

• PRESENTED AND COMPOSED A MANUSCRIPT ON THE RESEARCH CONDUCTED

PSU ANIMAL CELL AND TISSUE CULTURE METHODS AUGUST 2013 –

DECEMBER 2013

• LEARNED TO USE LAMINAR FLOW HOOD AND ASEPTIC TECHNIQUES

• PRIMARY CELL CULTURE FROM LIVING ORGANISM (CHICKEN EMBRYO) AND THEN VIEWED

DIFFERENT CELLS UNDER MICROSCOPE

• TESTED SUSPICIOUS CELLS FOR MYCOPLASMA CONTAMINATION AND THEN COMPARED IT TO

POSITIVE CONTROL

• FOLLOWED THE CHROMOSOME SPREADING PROTOCOL TO VIEW CHARACTERISTIC AND

DIFFERENT SIZES OF CHROMOSOME USING HELA CELLS

• FUSED TOGETHER BHK CELLS WITH MULTIPLE NUCLEI

PSU PLANT TISSUE CULTURE AND BIOTECHNOLOGY JANUARY 2014 –

MAY 2014

• MANIPULATED TOBACCO PLANTS BY ADDING AGROBACTERIUM AND OBSERVED IF TRANSGENIC PLANTS

EXPRESSED DESIRED GENES (NPTII AND GFP)

• PERFORMED SEED GERMINATION ASSAY

• PERFORMED AND CALCULATED ROOT EFFICIENCY IN TOBACCO PLANTS

• ISOLATED DNA FROM TRANSGENIC AND CONTROL PLANTS AND MEASURED DNA TRANSFORMATION WITH

PCR

• RAN THE SAMPLES THROUGH GEL TO CONFIRM RESULTS AND CHECK FOR ANY CONTAMINATION

Leadership

Biotechnology Club, Vice President August 2013 – May 2014

Planned and participated in the organizational fair to advertise the organization and welcome new members. Invited guest speakers

from the field to speak to students on campus about an exciting and different area of pharmaceutical and biotechnology field.

Hosted fund raiser at Quiznos sub and raised total $667 and received 15% of the total for club spending.

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