Jyothi Mula (Permanent resident) Mobile: 859-***-****

Email: acfudr@r.postjobfree.com

Lexington,KY

OBJECTIVE: To secure a position in the organization that offers challenge and opportunity for my career development and at the

same time serve the organization to the best of my capabilities. I would like to gain new skills while utilizing my current area of

expertise with in a positive team environment.

SUMMARY: Result oriented p rofessional w ith o ver 1 0 y ears of providing innovative technological solutions in the life

sciences and healthcare domain. Extensive qualifications in all aspects of M olecular biology, immunohistochemistry,

B iochemistry and some extent cell culture too. Articulate communicator who can fluently speak the languages of both

people and technology, blending technical expertise while interacting effectively with customers, clients and staff, and executive

management.

CORE COMPETENCIES

● Project Management.

● Working with different transgenic rodent models (helping in surgery and intraperitoneal administration of painkillers, antibiotics

after surgery).

● Protein isolation and estimation of tissue.

● Separation and purification of proteins using SDS gel electrophoresis and chromatography techniques.

● Performed different physiological experiments on blood, serum and urine samples

● Quantified proteins, lipids, carbohydrates, sugars, minerals, and ions in blood and urine.

● Western blot techniques

● Microbial techniques (developed different cultures, colonies of bacteria)

● Did different bacterial staining, like Grams Staining

● Extracted ethanol using fermentation process

CERTIFICATION

● HTL (Histotechnologist)

TRAINING

● Practical Project management.

● cGXP Compliance procedures.

● 21 CFR Part 11 Electronic Records Electronic Signatures Compliance.

EXPERIENCE AND ACCOMPLISHMENTS

Research Associate, Department of Rehabilitation sciences

University of Kentucky, Lexington, USA

AUG2009 Present

Rodent and Human Tissue Analysis:

• Human and rodent muscle tissue isolation, preservation and processing (both frozen and fixed

tissues).

• Frozen and paraffin muscle sectioning using microtome, vibrotome and cryostat.

• Develop complex immunohistochemcial and histochemical analysis methods.

• Troubleshoot problems associated with lab instruments and develop alternatives.

• Standardize protocols, maintain a detailed description of the protocols and procedures involved.

Image Analysis and Documentation:

• Computer-based photomicroscopic (fluorescent and brightfield microscopy) image acquisition and analysis.

• Quantification of computer based images.

• Compile data and perform statistical analysis.

• Writing manuscripts, technical abstracts and grants.

• Remain up to date with current, relevant technical literature.

Laboratory Supervision

Teach and supervise undergraduate and summer students, also training people from different research institutes.

Help students draft and submit manuscripts related to ongoing projects.

Senior Lab Technician, Department of Rehabilitation sciences

University of Kentucky, Lexington, USA OCT 2005 - AUG 2009

Anatomical analyses:

• Spinal cord tissue isolation, preservation, processing for confocal, and electron microscopic analysis.

• Immunohistochemistry labeling of excitatory and inhibitory neurotransmitter receptors and transporters

Protein analyses:

• Isolation and extraction of proteins from spinal cord tissue and determining protein concentration.

• Immunoblotting of protein extracts and signal quantification.

Lab Management:

• Troubleshoot protocols and help with the experimental design.

• Maintain detailed description of the protocols and procedures involved.

• Placing orders and maintaining the lab.

RESEARCH PUBLICATION

• Effective fiber hypertrophy in satellite cell-depleted skeletal muscle. Development. 2011 Sep;

138(17):3657-66, McCarthy JJ, Mula J, Miyazaki M, Erfani R, Garrison K, Farooqui AB, Srikuea R, Lawson BA,

Grimes B, Keller C, Van Zant G, Campbell KS, Esser KA, Dupont-Versteegden EE, Peterson CA

• Satellite cell depletion does not inhibit adult skeletal muscle regrowth following unloading-induced atrophy. Am J

Physiol Cell Physiol. 2012 Oct 303(8):C854-61. doi: 10.1152/ajpcell.00207.2012. Epub 2012 Aug 15 Jackson JR, Mula

JKirby TJ, Fry CS, Lee JD, Ubele MF, Campbell KS, McCarthy JJ, Peterson CA, Dupont-Versteegden EE

• Automated Image Analysis of Skeletal Muscle Fiber Cross-Sectional Area. J Appl Physiol. 2012 Nov 8 Mula J, Lee

JD, Liu F, Yang L, Peterson CA

• Automated Fiber Type Specific Cross-sectional Area Assessment and Myonuclei Counting in Skeletal Muscle. J

Appl Physiol (1985). 2013 Oct 3. Liu F, Fry CS, Mula J, Jackson JR, Lee JD, Peterson CA, Yang L.

• Fiber type-specific satellite cell response to aerobic training in sedentary adults.

Fry CS, Noehren B, Mula J, Ubele MF, Westgate PM, Kern PA, Peterson CA J Physiol. 2014 Apr 22. (Epub ahead of print)

• Satellite cell depletion alters the muscle environment, but not myofiber phenotype and function with aging

Christopher S. Fry, Jonah D. Lee, Jyothi Mula, Tyler J. Kirby, Janna R. Jackson, Fujun Liu, Lin Yang, Christopher L.

Mendias, Esther E. Dupont-Versteegden, John J. McCarthy, Charlotte A. Peterson.

(Accepted in Nature Medicine)

ABSTRACTS

• Sarcopenia is Independent of Lifelong Muscle Stem Cell Depletion

Jonah D. Lee1, Jyothi Mula1, Christopher S. Fry1, Janna R. Jackson1, Tyler J. Kirby2, Jake A. Beggs1, Marilyn S.

Campbell1, Catherine P. Starnes3, Esther E. Dupont-Versteegden1, John J. McCarthy2, Charlotte A. Peterson1

Presented at Center for Muscle Biology, University of Kentucky, Lexington, KY

• Satellite Cells are not a Prerequisite for Skeletal Muscle Regrowth Following Unloading-Induced Atrophy.

Jackson, J.R., Cry, C.S., Lee, J.D., Mula, J., Erfani, R.A., Kirby, T.J., Ubele, M.F., Lawson, B.A., Campbell, K.S., McCarthy,

J.J., Peterson, C.A., Dupong-Versteegden, E.E.

Presented at Experimental Biology, San Diego, CA, April 21-25, 2012.

• Anti-Inflammatory Muscle Macrophage Phenotype is Predictive of Resistance Training Gain in Older Individuals.

Lee, J.D., Erfani, R.A., Campbell, M.S., Mula, J., Peterson, C.A.

Presented at Experimental Biology, San Diego, CA, April 21-25, 2012.

TECHNICAL SKILLS

• Windows and Microsoft Office Suite, Visio and Microsoft Project

EDUCATION

• Post Graduate Diploma in Computer Applications (2002-2003) (JNNYC, Warangal, Andhra Pradesh, India)

• Master of Science (2000-2002): Biochemistry Major Kakatiya University, Warangal, Andhra Pradesh, India

• Bachelor of Science (1997-2000): Biochemistry, Microbiology, Chemistry Osmania University, Hyderabad, Andhra

Pradesh, India

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