LEILA HAIDARI
Home: ***-****** ******
Gatineau, QC. J8T 8N3
Tel: 819-***-****
E-mail: *********@*******.***
OBJECTIVE:
I am interested in applying for a position in Health Sciences/Cell Biology /Biochemistry/ Biotechnology-related field.
EDUCATION:
Master of Science in Molecular Biology and Genetics, Carleton University, August 2002.
Bachelor of Science, High Honours in Biology and Biotechnology, Carleton
University, April 1999.
ACADEMIC ACHIEVEMENTS:
First place prize for Ottawa Health Research Institute Poster
Presentation. 2007.
President’s Scholarship at Carleton University. 2000-2002.
Carleton University Graduate Student Bursary. 2000-2001.
Second place prize for Graduate Student Poster Presentation
at University of Ottawa. 2001.
Dean’s Honour‘s List at Carleton University. 1999.
NSERC Undergraduate Student Research Award. 1999.
Second place winner of the College of Natural Science (CNS)
poster competition at Carleton University. 1999.
Michael Oliver Scholarship. Carleton University. 1996.
Carleton University Entrance Scholarship. 1994.
Bloor Collegiate Institute (Toronto) Chemistry team
award. 1992.
Academic Achievement Award (over 90% average) for
grades 10-12, Danesh High school, Iran.1988-1990.
RESEARCH EXPERINCE:
Undergraduate Laboratory Assistance
Carleton University, Biology Department. May 1998 to Sept 1998.
As a part-time laboratory assistant for Landscape Ecology lab, I gained extensive experience working with different herbarium specimens. My responsibilities included data collection, data entry, cataloguing herbarium specimens, and some office work.
Laboratory Instructor
Carleton University, Chemistry Department. Sept 1998 to Apr 1999.
As a Chemistry first and second year lab demonstrator for three terms, I have gained enormous experience working with undergraduate students. I was responsible for supervising students, teaching the laboratory techniques and relevant theory, help in writing lab exams and grading of workbooks and formal reports, ordering lab materials and supplies; establishing and enforcing safety protocols for all laboratory settings, monitoring student progress and assigning laboratory grades.
B.Sc. Honours Student
Under supervision of Dr. James Cheetam, Carleton University. Sept 1998 - Apr 1999.
As an undergraduate student, I have done research on synapsin I, synaptic vesicles, liposomes and emulsions. I studied particularly the effects of calcium and ATP on synapsin binding to membranes as my honour's project.
Research Assistance
Under supervision of Dr. Myron Smith, Carleton University. May 1999 - Aug 1999.
As NSERC undergraduate student, I worked in Mycology lab on Neurospora crassa project. I was studying an osmotic-remedial, temperature-sensitive mutation in the allosteric activity site of ribonucleotde reductase in Neurospora crassa.
Teaching Assistance
Biology Department, Carleton University. Jan 2000 - Jan 2002.
During my graduate study, I worked as a teaching assistance in the following laboratories: Biochemistry, Plant Form and Function, Plant Physiology, Cell Biology and Genetics. I have developed strong communication skills through my extensive work with students. As well as successfully gained advanced scientific experience in various techniques, which are critical in modern plant Physiology, Biochemistry and molecular studies.
M. Sc. Student
Under supervision of Dr. Myron Smith, Carleton University. Sept 2000 - Aug 2002.
I worked on Neurospora crassa Project. I specifically investigated the heterokaryon incompatibility and expression of ribonucleotide reductase in Neurospora crassa as my M.Sc thesis.
Research Assistance
Under supervision of Dr. H.Chaim Birnboim. Ottawa Regional Cancer Center.
Feb 2002- Mar 2004.
I worked on two projects: The chemopredective diagnostic project associated with Roche company in Germany and the colon cancer tumor bank project. Also as a part of the research team, I was studying the mechanisms of resistance to Anti-Thymidylate Synthase chemotherapeutic agents in colon cancer.
Research Associates
Under supervision of Dr. Edward O'Brien. Ottawa Heart Institute. Oct 2004 - Aug 2005.
I worked on the Estrogen Receptors project. As part of the research team, I was studying the activity of estrogen receptors in the arteries of both males and females, using a gene discovery technique to look for novel proteins that may regulate these receptors.
Through a year of research at Ottawa Heart Institute, I was able to examine the interaction and cellular localization of Heat shock Protein (HSP27) and Estrogen Receptor-ß.
Research Assistance
Under supervision of Dr. Ken Garson, OHRI. Sept 2005 – Sept 2007.
I worked on few projects:
- Expression of Breast Cancer1 (BRCA1) and Excision repair cross complementation group (ERCC1) genes in Ovarian Cancer patients using QPCR.
- Ovarian Cancer cell purification from Ascites and Tumour cells.
- Isolation of a subpopulation in Ovarian Cancer-initiating cells using cells surface markers and side population by flow Cytometry.
Research Associate
Under supervision of Dr. Myron Smith, Health Canada. Feb 2009 – May 2009.
Studying the recovery of microorganisms as possible Biological Weapons in dust.
Food inspection and quality control manager
At SilkRoad Inc. June 2009- August 2014.
PROFESSIONAL ACTIVITY:
Conference and Workshop attended:
25th Annual Ottawa Reproductive Biology Workshop. Ottawa,
ON.2007.
24th Annual Ottawa Reproductive Biology Workshop. Ottawa,
ON.2006.
3rd National Conference on Ovarian Cancer Research. Vancouver,
BC. 2006.
Great Lakes-St. Lawrence Winter Workshop in Mycology. Toronto,
ON. 2001.
22nd Fungal Genetics Conference. Asilomar, CA. 2001.
44th Annual Meeting of the Canadian Genetic Society. Ottawa, ON.
2001.
Canadian Bioinformatics Resource, Basic Training. Ottawa, ON. 2001.
6th Ottawa Life Science National Conference Ottawa, ON. 1999.
PUBLICATIONS:
-Nonself Recognition Through Intermolecular Disulfide Bond Formation of Ribonucleotide Reductase in Neurospora. Genetics. 2013, 193(4):1175-83.
Smith, R.P., Wellman, K., Haidari, L., Masuda, H., Smith, M.L.
-The Interaction and Cellular Localization of HSP27 and Estrogen Receptor-ß are Modulated by 17ß-Estradiol and HSP27 Phosphorylation. Molecular & Cellular Endocrinology.2007, 270(1-2):33-42. Al-Madhoun, A.S., Haidari, L., Chen, Y.-X., Rayner, K., Gerthoffer,W., McBride, H., O'Brien, E.R.
LECTURE/PRESENTATIONS:
Lecture on:
- Leaves Variations, structure and function. 2nd year, Plant Form and Function course (61.202). Carleton University. 2001.
Oral Presentation on:
- Lipid rafts in cell membrane. Journal Club for Cell and Molecular Biology,
Carleton University. 2001.
- Structural insight into the molecular mechanism of calcium-dependent
exocytosis. The college of Natural Sciences, Carleton University. 2001.
- Non-self recognition in fungi. 4th Bio-Medical Research Day, Carleton
University. 2000.
- Cancer chemo preventive activity of synthetic colorants used in foods,
pharmaceuticals and cosmetic preparations. Biology Departmental Seminar,
Carleton University. 2000.
Poster Presentation on:
- Low BRCA1 and ERCC1 Expression Correlates with Improved Survival in Ovarian Cancer. AACR-NCI-EORTC Conference on Molecular Targets & Cancer Therapeutics, San Francisco. 2007.
- Targeting the BRCA1-dependent DNA damage and repair pathway in sporadic
epithelial ovarian cancer. Ottawa Health Research Institute. 2007.
- Heterokaryon incompatibility associated with the large subunit of ribonucleotide
reductase in Neurospora crassa. Asilomar, CA. 2003.
- The effects of calcium and ATP on synapsin I-induced aggregation of vesicles. The
College of Natural Sciences Research Day, Carleton University. 1999.
- Carnivorous plants. Ecology Poster Day, Carleton University. 1999.
TECHNICAL SKILLS:
Cell & tissue culture techniques
Cell purification from primary tissues (Tumour and ascites)
Colony growing in Methylcellulose and Soft agar techniques
Bacterial and fungal basic culture maintenance
Fungal genomic DNA extraction
DNA & RNA extraction from tissues embedded in paraffin
Extraction of Protein, DNA and RNA from cells or tissues
Cloning of bacterial & mammalian constructs
DNA & RNA gel electrophoresis
Bacterial transformation
PCR & RT-PCR techniques
Q-PCR (Real time PCR)
Southern blotting
Western blotting
Preparation of Liposomes using the LiposoFast Pneumatic devise
Phosphate assay
Protein assay: Lowry, Bradford, UV280
Spectrophotometric or colorimetric assay
Protein extraction and purification: gel filtration, affinity absorption
. Immunofluorescence & Immunohistochemistry
. Light microscopy & Confocal microscopy
Cell preparation for Flow Cytometry
Use and handling of radioisotopes (3H, 35S, 32P)
INTERPERSONAL AND COMMUNICATION SKILLS:
Listen actively and attentively
. Solve problems logically, creatively, and practically
Writing concisely
Delegate tasks and responsibilities
Understand and work within the group culture
Display understanding of, and respect for, people from diverse backgrounds
Express ideas and thoughts based on facts
COMPUTER SKILLS:
. Microsoft: Word, Excel, PowerPoint, Internet Explorer & Outlook Explorer
. Corel: WordPerfect, Quattro Pro
. Corel Draw: Corel Photo-Paint, Adobe Photoshop
. Graph Pad Prism, DNA Star, Primer Premier, Reference Manager
. Relative quantification analysis, using Lightcycler software
VOLUNTEER EXPERIENCE:
Worked as volunteer graduate student in Molecular Biology Laboratory for Carleton’s
Annual University Day. 2001.
Worked as volunteer student at the Information Desk for the Canadian Federation of
Biological Societies Meeting. Congress Center, Ottawa.2001.
Worked in Administration at Queensway-Carleton Hospital. Summer 1997 as volunteer
student.
Worked in Children Cancer center at Toronto Children Hospital. Summer 1993 as
volunteer student.
OTHER EXPERIENCE:
Ten days training on quantitative Real time PCR (LightCycler) in Germany. Winter
2002.
LANGUAGES SPOKEN:
- English
- Persian
PERSONAL INTEREST:
- Reading
- Music
REFERENCS:
Available upon request.