Medical Technologist

SESHAGIRI R. DUVVURI

**** * ********* **** ****: 541-***-****

Houston, TX 77096 Email: *********.*.*******@*****.***

Summary

Highly motivated and detail-oriented Senior Research Assistant with proven expertise in Flow

Cytometry. Self-driven and quick to grasp new techniques.

7 years of experience in Flow Cytometry including setting up new flow protocols (BD Calibur,

LSR-II, BC-Galios).

Significant experience in analyzing data with Flowjo and Kaluza.

Significant experience in cell and tissue culture, Western blots, RT-PCR, microarrays and

associated data analysis. Also have experience with ELISA, RNA and DNA isolation and

qPCR.

Excellent written and oral communication skills as well as interpersonal skills.

Collaborated with different groups such as Statistics, Bioinformatics, and other labs within

Leukemia department at MD Anderson.

Presented research results to peers in lab meetings on a regular basis and presented posters in

conferences.

Trained new/existing personnel (Ph.D students, physicians and other technicians) on Flow

Cytometry and mentored, as needed.

Assumed lab management responsibilities in the absence of the lab manager.

Experience

Graduate Research Asst. Dept. of Biology and Biochemistry, Univ of Houston (Jan 2014 - Present)

Evaluated the effect of micro-RNAs on Ovarian cancer cell lines.

Studied the effect of micro-RNAs in sensitizing cancer cells to various drugs including Cisplatin

and ABT-737.

Senior Research Asst., Leukemia Department, MD Anderson Cancer Center (Mar 2011 - Aug 2013)

Crucial part of a two member team that worked on two clinical trials.

“Clinical trial to evaluate the effect of an MDM2 inhibitor in AML and CLL patients-Phase-I and II”

Processed the blood samples (Ficoll and/or RBC lysis)

Stained for up to 7 markers on single samples and analyzing the effects on all cell surface

markers tested.

Tested an autophagy marker. Measured the autophagy marker on a BD LSR-II and the rest on a

Beckman Coulter Galios.

Data analysis using Kaluza.

“Clinical trial to determine the effect of a CXCR4 inhibitor and G-CSF with a Flt-3 inhibitor in patients with

CML”

Analyzed blood samples collected from patients for changes in various stem cell markers and

adhesion molecules using flow cytometry. Data Analysis using Kaluza.

Analyzed all samples using a Beckman Coulter Galios.

Research Asst. II, Stem Cell Transplantation and Cellular Therapy, MD Anderson Cancer Center

(Mar 2007-Mar 2011)

“Effect of MDM2 inhibitors in AML, CLL, CML, Lymphoma” (Dr. Michael Andreeff and Dr. Gautam

Borthakur)

Evaluated a novel MDM2 inhibitor in AML, CLL, CML and Lymphoma

1

SESHAGIRI R. DUVVURI

5503 S Braeswood Blvd Cell: 541-***-****

Houston, TX 77096 Email: *********.*.*******@*****.***

Provided input on experimental design and suggested additional experiments during project

design.

FACS analysis of both p53 WT and mutant cell lines treated with the inhibitor, western blot

analysis and RT-PCR

“Notch signaling pathways in Leukemia.” (Dr. Marina Konopleva and Dr. Michael Andreeff)

Analyzed AML cell lines to determine the effect of Notch signaling and the effect of an

inhibitor on the Notch signaling pathway

Used flow cytometry, western blot analysis and RT-PCR in this study

Assisted colleagues in other projects by conducting experiments as needed. Projects involved

investigating the role of p53 in CLL, CRM1 and B55- in AML

Graduate Research Asst. (MS) Linus Pauling Institute, Oregon State University (Dec 2001 – Jun

2006)

Research Advisors: Dr. Balz Frei and Dr. Tory Hagen

“Effect of micronutrients on Gene Expression in vitro and in vivo using Microarrays.”

“Changes in gene expression due to aging in rat liver and effect of dietary supplementation of alpha-

lipoic acid.”

“Effect of ascorbic acid on gene expression in human aortic endothelial cells exposed to low levels of

lipopolysaccharide.”

Techniques included, DNA microarray, associated data analyses and pathway analysis using

software such as Genespring, pathway assist and Metacore.

Research Asst, Dept of Biochemistry and Biophysics,Oregon State University (Jun 1999 – Nov

2001)

“Crystallographic analysis of structures of Old Yellow Enzyme mutants.”

Mutants of Old Yellow Enzyme were analyzed using X-ray crystallography.

Research Assistant, Center for Biotechnology, Anna University, India (Jun 1997 – May 1998)

Master’s Thesis, Dr. A.L. Mudaliar Institute of Basic Medical Sciences, University of Madras, In

(Sep 1995 – May 1997)

Research Advisor: Dr. Radha Shanmugasundaram

“Effect of cigarette smoke on germinating seeds of Cicer Arietenum”

Technical Expertise

Biochemistry, Molecular Biology and Cell Biology

Flow Cytometry: BD Calibur, LSR-II, BC-Galios.

Enzyme isolation and purification, enzyme assays, SDS PAGE, Western blotting, flow cytometry,

mammalian cell culture, isolation and culture of primary rat hepatocytes, cell viability assays, RNA

isolation, DNA isolation and RT-PCR

Analytical

HPLC, size exclusion chromatography, affinity chromatography, thin layer chromatography, UV-Vis

spectroscopy and fluorescence spectroscopy

Data Analysis

Analysis of microarray data (Genespring and Array Assist), Prism Graph, Metacore - pathway analysis

software

Computer Skills

2

SESHAGIRI R. DUVVURI

5503 S Braeswood Blvd Cell: 541-***-****

Houston, TX 77096 Email: *********.*.*******@*****.***

Windows XP, 7, 8 Office 10, MAC OSX

Education

M.S. Biochemistry and Biophysics, Oregon State University, 2006

M.Sc. Biochemistry, University of Madras, India, 1997

B.Sc. Biochemistry, Jaya College of Arts and Science, India, 1995

Publications

Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V. The role of glutamine 114 in old

yellow enzyme. J Biol Chem. 2002 Jan 18; 277(3): 2138-45 (Research Article).

Samudio I, Harmancey R, Fiegl M, Kantarjian H, Konopleva M, Korchin B, Kaluarachchi K,

Bornmann W, Duvvuri S, Taegtmeyer H, Andreeff M. Pharmacologic inhibition of fatty acid

oxidation sensitizes human leukemia cells to apoptosis induction. J Clin Invest. 2010

Jan;120(1):142-56.

Activation of p53 signaling by MI-63 induces apoptosis in acute myelogenous leukemia cells.

Ismael J Samudio, Seshagiri Duvvuri, Julie C Watt, Duncan Mak, Hagop Kantarjian, Dajun Yang,

Vivian Ruvolo and Gautam Borthakur. Leukemia and Lymphoma 2010.

Decreased sensitivity of 17p-deleted chronic lymphocytic leukemia cells to a small molecule BCL-

2 antagonist ABT-737. Kojima K, Duvvuri S, Ruvolo V, Samaniego F, Younes A, Andreeff M.

Cancer 2011.

Prognostic impact and targeting of CRM1 in acute myeloid leukemia. Kensuke Kojima, Steven M.

Kornblau, Vivian Ruvolo, Archana Dilip, Seshagiri Duvvuri, R. Eric Davis, Min Zhang, Zhiqiang

Wang, Kevin R. Coombes, Nianxiang Zhang, Yi Hua Qiu, Jared K. Burks, Hagop KantarjianSharon

Shacham, Michael Kauffman and Michael Andreeff. Blood 2013

The protein phosphatase 2A regulatory subunit B55α is a modulator of signaling and microRNA

expression in acute myeloid leukemia cells. Ruvolo PP, Ruvolo VR, Jacamo R, Burks JK, Zheng Z,

Duvvuri SR, Zhou L, Qiu Y, Coombes KR, Zhang N, Yoo SY, Pan R, Hail N Jr, Konopleva M, Calin

G, Kornblau SM, Andreeff M.Biochim Biophys Acta. 2014 May 21. pii: S0167-4889(14)00153-0

Presentations

Seminar on “Analysis of gene expression modulated by Vitamin C using microarrays”, Annual Linus

Pauling Institute Retreat. (2003)

Seminar on “Structure of mutant Old Yellow Enzyme”, Department of Biochemistry and Biophysics,

Oregon State University (2000)

Poster on “Effect of ascorbic acid on gene expression in human aortic endothelial cells exposed to

low levels of LPS”, Society for Free Radicals in Biology and Medicine Conference, Austin, Texas

(2005)

Poster on “Effect of Nutlin-3a and or SAHA in AML”. American Society of Hematology, New Orleans,

Dec 2009.

Poster on “Effect of nutlin-3a on Autophagy in Leukemia cells”. American Society of Hematology,

Orlando, Dec 2010.

Awards and Honors

Recipient of Exceptional Performance award, MD Anderson Cancer Center, Houston,TX (2009,

2010)

3

SESHAGIRI R. DUVVURI

5503 S Braeswood Blvd Cell: 541-***-****

Houston, TX 77096 Email: *********.*.*******@*****.***

Recipient of the ICAR (Indian Council for Agricultural Research) Fellowship for research towards the

Master’s degree (1995)

Recipient of the CSIR (Council for Scientific and Industrial Research, Govt. of India) Fellowship for

pursuing a graduate degree (1997)

Best Outgoing Student award, Jaya College of Arts and Sciences, India (1995)

References

Available upon request

4

Contact this candidate