Research Technologist

Aparajita Mukherjee (Basu)

SUMMARY:

. Over * years of laboratory experience in oncology, molecular biology

and cell culture.

. Exceptional organization skills, strong analytical and problem solving

skills.

. Data collection, interpretation and analysis to prepare reports.

. Managed lab supplies and conducted equipment maintenance &

troubleshooting.

. Mentored and supervised undergraduate students and research trainees.

. Self-motivated team player working well in collaborative multifunctional

groups.

. Experienced with laboratory instruments including StepOnePlus (Applied

BioSystems), Microplate Reader (Tecan), Incucyte Live Cell Content

Imager (Essen Bioscience), Accuri C6 (BD Biosciences), Countess

Automated Cell Counter (Life Technologies), Invivo BioImaging (Xenogen)

etc.

. Proficient in Microsoft Office tools (WORD/EXCEL/POWERPOINT) and

data analysis software like SigmaPlot, TECAN iControl, FlowJo, mouse

Laboratory Information Management System (mLIMS) for data entry.

EDUCATION:

Master of Sciences (MS) Microbiology 1999 SRT

Marathwada University, Nanded, INDIA.

Bachelor of Sciences (BS), Microbiology 1997

Marathwada University, Aurangabad, INDIA.

RESEARCH EXPERIENCE:

Research Technologist II (June 2012 - Jan 2014) at the Department of

Pharmacology and Toxicology (Cancer Center), Medical College of Wisconsin,

Milwaukee, WI.

. Cell Culture: Cell-based assays to analyze cell growth, viability and

apoptosis for mammalian (lung) tumor cell lines (A549, H1299, H292,

H441, Hcc827, H1975, LLC, PCC4, PhxA, Beas2B), Trasfection of cell

lines, beta-galactosidase and luciferase assay, MTS assay, Cell

Migration and Invasion Assay, Immunofluorescence, Flow Cytometry.

. Animal Handling: Breeding, Genotyping, Subcutaneous Injection of lung

tumor cells, Injection of chemokines and monitoring tumor regression

manually by calipers and biophotonic imaging

. Molecular Biology: Plasmid isolation, cloning, chemical transformation,

PCR, agarose gel electrophoresis, RNA isolation from cells, tissues and

tumors, miRNA isolation from cells, RT-PCR, qPCR to analyze gene

expression.

. Protein analysis: Isolation of proteins from cell lines and tumors,

BCA protein assay, SDS-PAGE, Western Blot, ELISA.

Research Technician at Massachusetts General Hospital, (April 2005 - May

2006) at the Department of Surgery (Microbiology and Immunology), Harvard

Medical School, Boston, MA.

. Cell Culture: Viability assays on mammalian (prostate) tumor cell lines

(LNCaP, DU-145, TRAMP) following treatment with chemotherapeutic drugs

and Herpes Simplex Virus (HSV) to determine synergy using MTT and MTS

assay.

. Animal Handling: Breeding, Genotyping, Mouse Tumor Xenograft, Injection

of prostate tumor cells, analysis of tumor regression by synergy

between chemotherapeutic agents and HSV.

. Virology: HSV Purification and quantitation (titration) by Plaque

formation assay.

Project (Technical) Assistant (April 2001 - June 2004) at the Department of

Exploration of Microbial Biodiversity, Institute of Microbial Technology,

Chandigarh, India.

. Microbiology: All basic techniques including isolation,

characterization and preservation of bacterial strains, Physical and

Chemical Mutagenesis, Characterization of various metabolic pathways

through study of intermediate and end-products.

. Molecular Biology: Isolation and purification of chromosomal and

plasmid DNA from various Gram positive and Gram negative strains,

restriction digestion, ligation, electroporation and chemical

transformation in bacteria, PCR, agarose gel electrophoresis, Sample

preparation for DNA sequencing, Southern hybridization, DNA dot blot

and colony hybridization, Transposon-mediated mutagenesis and Amplified

rDNA Restriction Analysis (ARDRA).

. Analytical Techniques: Solvent extraction of metabolites, Thin Layer

Chromatography, Fatty acid methyl ester analysis for bacterial

identification by gas chromatography (GC).

PEER-REVIEWED RESEARCH PUBLICATIONS (cited over 100 times as per Google

Scholar):

(http://scholar.google.com/citations?user=pde8NvoAAAAJ&hl=en&oi=ao)

. Ruchika Budhiraja, Aparajita Basu and Rakesh K. Jain (2002). Microbial

diversity: Significance, conservation and application. Science Letters

2002; 25, 189-201.

. Sumeet Labana; Om Vir Singh; Aparajita Basu, Gunjan Pandey and Rakesh

K. Jain (2005). A microcosm study on bioremediation of p-nitrophenol

contaminated soil using Arthrobacter protophormiae RKJ100. Applied

Microbiology and Biotechnology 2005; 68(3): 417-24.

. Sumeet Labana; Gunjan Pandey; Debarati Paul; Narinder K. Sharma;

Aparajita Basu and Rakesh K. Jain (2005). Pot and Field Studies on

bioremediation of p-nitrophenol contaminated soil using Arthrobacter

protophormiae RKJ100. Environmental Science and Technology 2005; 39(9):

3330-7.

References available on request. US Permanent Resident (Green Card

Holder) : Authorized to work for any employer without visa sponsorship

9 Madison Lane

Acton, MA 01720

Email: *********.*********@*****.***

Cellphone: 414-***-****

Contact this candidate