High School Pvt Ltd

SUNITH. KN

Masters in Biochemistry (M Sc)

Contact détails : **************@*****.***

Tel; +91-973*******,973-***-****.

OBJECTIVE:

An aspiring future researcher, very eager and keen to utilize my knowledge and skills

in pursuing a successful career in Life sciences research areas.

KEY TOPICS UNDERTAKEN SO FAR:

Cell Biology & Molecular Biology

Enzymology

Human & Molecular Physiology.

Bio-Chemical Genetics & Biotechnology

Bioinformatics

Immunology & Clinical Biochemistry

PRACTICAL EXCERSISES PERFORMED

Mammalian Cell culture Techniques & its Assays performed

• Media preparation & maintenance of aseptic techniques

• Suspension and adherent cell cultures : Sub-culturing of cells and Cell counting

• Maintaining a cell line, freezing, thawing and proliferative studies.

• Qualitative & quantitative assay of a drug.

Molecular Biology Techniques

• Isolation and quantification of DNA (genomic and plasmid) from bacteria, plant,animal

sources, blood and also RNA

• Agarose gel electrophoresis and DNA damage studies by Comet analysis

• SDS PAGE

• Southern Blotting, Northern Blotting & Western Blotting

• Restriction Digestion, Transformation and Ligation studies in Bacteria

• PCR (Basic)

Biochemical Techniques

• Qualitative and Quantitative analysis of Nucleic Acid

• Protein Isolation and Buffer Preparation

• Estimation of Protein by Bradford’s and Lowry’s Method

• Chromatographic techniques: TLC, Column Chromatography, Paper Chromatography.

Immunological Techniques.

• All types of ELISA, precipitation and agglutination techniques, Immunoblotting

• Rocket Immunoelectrophresis, Double immune diffusion (Ouchterlony’s method).

Basic Microbiological Techniques

• Media Preparation, Isolation of bacteria/fungus from soil sample

• Culturing of Microbes by different streaking methods

• Primary and secondary screening of active microbes

• Antibiotic sensitivity Test, MDR Test, Minimum Inhibitory Concentration (MIC) Test.

Plant Tissue culture

Animal Rearing and Dissection:

• Maintenance of experimental animals (rat, chick) and extraction of enzymes Alpha

Glucosidase and Aldose reductase from rat.

INSTRUMENTS HANDLED

• UV/Visible Spectrophotometer

• Colorimeter, pH meter, and Shaker incubator

• PCR (Thermo cycler) – Basic

• Centrifuge (Ultra,Table Top and Cooling centrifuge)

• Electrophoresis apparatus (Agarose & SDS)

• All types of Micropipettes

• Laminar Air Flow

• Microscopes (Compound & Research)

EDUCATION:

Percentage

Examinatio School/college University/Board Specialization Year of

%

n Passing

CMR Institute of

Management BIOCHEMISTRY. 2014 72.575

Bangalore

MSc

Studies Bangalore

Government CHEMISRTY,

Science College 2012

Bangalore

BSc BOTANY,

Bangalore 75

ZOOLOGY.

Pre University

Sindhi College, Education

PCMB 66

PUC 2009

Hebbal,Bangalore Karnataka.

Sheshadripuram Karnataka

High School Secondary GENERAL 2007 83.20

SSLC

Yelahanka Education

Bangalore. Examination Board

Project work:

The Dissertation work done at Skanda Life Sciences Pvt Ltd, Bangalore mainly on

Cell biology (Cell based assays, Biochemical assays & Microbiological assays ). My research

topic is on “Screening of selected medicinal plant extracts for Anti-diabetic

activity by In-vitro methods”.

Marks obtained: - 91/100.

PRESENTATIONS:

Poster Presentation;-

Lalitha.V, Sunith K .N, Kiran.B, Raveesha.K.A .In vitro evaluation of antifungal activity of

oils extracted from medicinal plants against seed borne fungi of paddy . National Conference

On “FRONTIERS AND CHALLENGES IN CHEMISTRY” held on October 10-11, 2013 at

Department of Chemistry,Don Bosco Institute of Technology, Mysore Road, Bangalore – 560

074.

WORKSHOPS/SYMPOSIA PARTICIPATION:

1. National Conference on “Biomedical spheres of life and life sciences” Conducted by CMR

Institute of Management Studies, Bangalore On 6th March 2013.

2. Science Academies Lecture Workshop on “ MICROBIAL GENOMICS” Conducted by

Maharani Science College For Women, Bangalore-56001. On 19th-20th September, 2013

3. Science Academies Lecture Workshop on ‘’ Enkindle intelligentsia’’ Conducted by CMR

Institute Of Management Studies, Bangalore On 22nd - 23rd January 2014.

REFERENCES:

• DR. KRISHNA KISHORE, ASST PROFESSER, DEPARTMENT OF BIO-

CHEMISTRY, CMRIMS, BANGALORE-57. Email: ******************@*****.***

Ph.No:094********

• Mr. ABDUL NASAR, ASST PROFESSER, DEPARTMENT OF BIO-CHEMISTRY,

CMRIMS, BANGALORE-57. Email: ********@*****.*** Ph.No:897*******

• DR. YOGISHA.S., DIRECTOR AND FOUNDER, SKANDA LIFESCIENCES

PVT.LTD, BANGALORE – 91. Email; *******.*@******************.***

Ph No: 096********

I hereby declare that all the statements made above are true, complete and

correct to the best of my knowledge and belief.

Date:

Place: Signature of the candidate

Contact this candidate