Technician University
Resume:
Hasina A. Ambia-Sobhan
******.*****.******@*****.***
Career Profile
Results-oriented biochemist with scientific expertise in biochemical research and expertise in
biocompatibility/protein adsorption. Expertise in biological sample handling, preparation of microbial
growth culture media, experienced with bacterial and mammalian cell culture and have current
experience in a biotech industry in assay development on assessing lead polymer biocompatibility/protein
adsorption and over twenty years of experience in academic biological research lab.
Qualifications
• Experienced, dependable, energetic scientist who puts on extra effort in assignments
• Excellent interpersonal communication skills
• Adapts quickly to changes in priorities to meet needs of supervisor, department and company
• Highly motivated and ability to multi-task and work independently or in a team environment
• Experienced with ELISA development
• Experienced with aseptic technique, cell culture, growing E.coli and biological sample handling
• Experienced with HPLC, GC, and wet chemistry techniques, ELISA, RIA assays
• Experienced with conducting small animal body composition using Lunar DXA and PIXIMUS
instruments.
• Experienced with giving hands on training to undergraduate students in the Organic and Inorganic
Labs
Professional Experience
Research Volunteer, PEG Biosciences Inc. August 11, 2014 - Present
Monmouth Junction, NJ
• Responsibilities: Kinetic study on Pegylated proteins, purification and analysis of hydrolysates on
HPLC (Shimadzu HPLC, LabSolutions/LCsolution Operation).
Associate Scientist, Cytosorbents, Inc., Biology Department August 2013- May 2014
Monmouth Junction, NJ
• Responsible for conducting biological assays to assess polymer hemocompatibility as well as
adsorption. Assays include protein quantification (ELISA Bio-Tek plate reader), hemocompatibility
(Helena Cascade M), assay development, and running SDS-PAGE electrophoresis (Novex-
Biotech). Assays were performed for advancement of lead polymers.
Hasina A Ambia-Sobhan 6707 Shadow Oaks Court, Monmouth Junction NJ 08852
Hasina A. Ambia-Sobhan
******.*****.******@*****.***
• Generate datasets that are instrumental in making polymer advancement decisions.
• Responsible for inventory and purchasing.
Research Volunteer, Department of Nutritional Sciences October 2012- July 2013
Rutgers University, New Brunswick, NJ
• Responsible for preparation and sterilization of culture media, weighed, measured, and worked
with chemicals, maintained MSDS and associated documentation.
• Responsible for growing E. coli for recombinant protein extraction and also performed cell culture
• Purified recombinant mammalian proteins from E. Coli using ion exchange resin chromatography,
affinity chromatography, SDS-PAGE electrophoresis, western blotting
Researcher III, Department of Nutritional Sciences July 1998 – December 2012
Rutgers University, New Brunswick, NJ
• Responsible for Biological samples handling such as human blood, urine collection, aliquoting,
storing in freezers and retrieving samples for biochemical analysis.
• Responsible for HPLC (Hitachi-High Technologies) analysis of hydrolyzed and purified urine
samples via preparative column chromatography, creatinine assay on plate reader (Molecular
Devises Versa-Max) and analysis of mineral components in human urine by Atomic Absorption
Spectrophotometry (Thermo-Fisher)
• Responsible for running human serum ELISAs (Molecular Devices UV/VIS) based on
immunoassays and radio-immuno assays (RIA, Perkin-Elmer gamma counter) to support clinical
study objectives.
• Responsible for isolating DNA from human whole blood, PCR amplification of DNA, running DNA
on agarose gel to assess polymorphism of vitamin D receptors and estrogen receptors.
• Responsible for conducting RT-qPCR and western blot analysis to quantify mice intestinal calcium
transporters, analyzing mice serum (RIA), urine (quantified collagen cross-links) and feces
(quantified total calcium by plate reader, muffle furnace).
• Studies include injecting mice with radioactive calcium-45 or saline, collecting whole blood by eye
bleeding or heart puncture for further analysis (serum osteocalcin, PTH), extracting mice intestinal
mucosal cells to isolate RNA, cDNA synthesis and to run RT-qPCR, collecting 5-day urine for
analysis (collagen cross-link analysis by running on HPLC), collecting 5-day feces and ashing in
muffle furnace, reconstituting ash and quantifying total calcium excretion and endogenous
Hasina A Ambia-Sobhan 6707 Shadow Oaks Court, Monmouth Junction NJ 08852
Hasina A. Ambia-Sobhan
******.*****.******@*****.***
radioactive calcium-45 excretion (Scintillation counter) in feces to determine percent true calcium
absorption (TRCA) from food.
Senior Laboratory Technician, Department of Nutritional Sciences October 1990 – July 1998
Rutgers University, New Brunswick, NJ
• Responsible for analysis of chicken growth plate cartilage implementing several techniques such
as tissue culture, in situ hybridization, radioisotope (S-35) tracer techniques (Perkin-Elmer
scintillation counter), PD-10 column chromatography, CL-2B sepharose column chromatography,
hydroxyproline assay and glycosaminoglycan assay, creatinine assay (Bio-Rad plate reader).
• Responsible for analyzing human and rat urine collagen cross-links by hydrolysis, running CF1-
cellulose preparative column chromatography, HPLC (Waters Millennium) and creatinine assays
(Bio-Rad reader plate) to asses bone resorption.
• Responsible for measurement of body composition of small animals (rats and mice) with x-ray
instruments such as Lunar DXA instruments and PIXIMUS instruments.
Laboratory Technician, Center of Alcohol Studies January 1988 – October 1990
Rutgers University, New Brunswick, NJ
• Responsible for running breathe test of alcohol treated rats to determine rat blood alcohol level
by gas chromatography; behavioral studies of alcoholic rats on maze and analysis of
adenylatecyclase enzyme and B-receptor binding in heart muscle tissue of alcoholic rats by
radioactive tracer (P-32) techniques. Study includes inserting cannula in rat stomach; alcohol
infusion to rat once a day for one to three weeks and running breathe test and heart muscle
collection.
Laboratroy Instructor, Organic and Inorganic Labs, Biochemistry July 1983 - July 1987
Dhaka University, Bangladesh
• Responsible for hands on train students in wet chemistry and organic chemistry laboratory
EDUCATION AND CERTIFICTIONS
BSc/MSc Biochemistry, Dhaka University, Bangladesh
Occupational Safety & Health Administration (OSHA) General Industry Certification Certificate Program,
UMDNJ, 2013
40-Hour Training and Certification in Health & Safety for Hazardous Waste Site Investigation, 2013
In-House Clinical Research Associate certification (CRA) courses, Qtech Solutions, 2013
Hasina A Ambia-Sobhan 6707 Shadow Oaks Court, Monmouth Junction NJ 08852
Hasina A. Ambia-Sobhan
******.*****.******@*****.***
Continuing Education courses in Advanced Nutrition, Rutgers University, 1988 - 1991
ADDITIONAL SKILLS
Laboratory: Biological laboratory equipment including HPLC, Gamma Counter, Micro plate readers
(BioTek reader plate and Molecular Devices reader plate, Bio-Rad plate reader), Scintillation counter
(Perkin Elmer), Gamma counter (Perkin-Elmer), Atomic Absorption Instrument (Thermo Scientific), Muffle
Furnace, PCR and RT-PCR instruments, light microscopy
Software: Microsoft Office 2010, Power Point, GraphPad, SoftMax Pro, Food Works
Hasina A Ambia-Sobhan 6707 Shadow Oaks Court, Monmouth Junction NJ 08852
Hasina A. Ambia-Sobhan
******.*****.******@*****.***
Publications:
1. Areal and volumetric bone mineral density and geometry at two levels of protein intake during
caloric restriction: a randomized, controlled trial. Sukumar D, Ambia-Sobhan H, Zurfluh R,
Schlussel Y, Stahl TJ, Gordon CL, Shapses SA. J Bone Miner Res. 2011 Jun;26(6):1339-48. doi:
10.1002/jbmr. 318
2. Energy restriction is associated with lower bone mineral density of the tibia and femur in lean but
not obese female rats. Hawkins J, Cifuentes M, Pleshko NL, Ambia-Sobhan H, Shapses SA. J
Nutr. 2010 Jan;140(1):31-7. Epub 2009 Nov 18. Erratum in: J Nutr. 2010 Apr;140(4):877.
3. Bone mineral density and content during weight cycling in female rats: effects of dietary amylase-
resistant starch. Bogden JD, Kemp FW, Huang AE, Shapses SA, Ambia-Sobhan H, Jagpal S,
Brown IL, Birkett AM. Nutr Metab (Lond). 2008 Nov 26;5:34.
4. Blood lead levels and bone turnover with weight reduction in women. Riedt CS, Buckley BT, Brolin
RE, Ambia-Sobhan H, Rhoads GG, Shapses SA. J Expo Sci Environ Epidemiol. 2009
Jan;19(1):90-6. Epub 2008 Mar 5.
5. Effects of formalin fixation and collagen cross-linking on T2 and magnetization transfer in bovine
nasal cartilage. Fishbein KW, Gluzband YA, Kaku M, Ambia-Sobhan H, Shapses SA, Yamauchi M,
Spencer RG. Magn Reson Med. 2007 Jun;57(6):1000-11.
6. Premenopausal overweight women do not lose bone during moderate weight loss with adequate
or higher calcium intake. Riedt CS, Schlussel Y, von Thun N, Ambia-Sobhan H, Stahl T, Field MP,
Sherrell RM, Shapses SA. Am J Clin Nutr. 2007 Apr;85(4):972-80. Erratum in: Am J Clin Nutr.
2007 Sep;86(3):808.
7. Overweight postmenopausal women lose bone with moderate weight reduction and 1 g/day
calcium intake. Riedt CS, Cifuentes M, Stahl T, Chowdhury HA, Schlussel Y, Shapses SA.J Bone
Miner Res. 2005 Mar;20(3):455-63. Epub 2004 Nov 29.
8. Bone and gastric bypass surgery: effects of dietary calcium and vitamin D. Goode LR, Brolin RE,
Chowdhury HA, Shapses SA.Obes Res. 2004 Jan;12(1):40-7.
9. Energy restriction reduces fractional calcium absorption in mature obese and lean rats. Cifuentes
M, Morano AB, Chowdhury HA, Shapses SA. J Nutr. 2002 Sep;132(9):2660-6.
10. Chronic ethanol treatment of rats and the myocardial beta-adrenoceptors. Pohorecky LA, Hamm
M, Chaudhury H, Ponticiello R, Knapp DJ. Alcohol. 1992 Jul-Aug;9(4):305-9.
11. Harun K. M. Yusuf, Sabiha Rahman, Hasina Akhtar: Rats Kept in Social Isolation for Twelve
Months from Weaning: Performance in Maze Learning and Visual Discrimination Tests, and Brain
Composition. Int. J. Neural Syst. 1(2): 143-147 (1989)
12. Dissertation: Peanut oil cake residue as a source of protein and associated biochemical studies. A
dissertation submitted to the University of Dacca in the partial fulfillment for the degree of Master
of Science in Biochemistry. Department of Biochemistry and Nutrition, University of Dacca, July
1974. Author: Hasina Akhter, Advisor: Dr. Ashraful Alam.
Hasina A Ambia-Sobhan 6707 Shadow Oaks Court, Monmouth Junction NJ 08852
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