Masters in Bioengineering from UC San Diego

Summary: To find a research position in biotechnology using my hands-on

laboratory experience and critical thinking as well as

communication/teamwork skills and further develop my knowledge in

improving healthcare.

Education:

9/2012 - 04/2014: University of California, San Diego: La Jolla, California

M.Eng in Bioengineering, Graduation: March 2014

GPA: 3.77/4.0

9/2007 - 7/2011: RV College of Engineering (VTU): Bangalore, India

B.E in Biotechnology, Graduation: July 2011

GPA: 8.89/10, Rank: 10 out of 12

(Thesis: "In vitro culture studies in cotton plant")

(Gold medalist in Pharmaceutical Biotech)

Academic Experience:

UCSD Department of Bioengineering and Department of Chemical Engineering

01/ 2013-06/ 2013

Graduate Teaching Assistant and Graduate Reader

. Assisted with the undergraduate Bioengineering Laboratory Course and

Mechanical Engineering Thermodynamics classes with discussion sections

for a class of 70 students

. Used strong communication skills to communicate concepts clearly and

effectively to students.

UCSD Moores Cancer Center - Tumor Immunology Laboratory

04/2014-Present

Adviser: Dr. Maurizio Zanetti

. Carcinoma studies conducted on human cell lines like PC3, U937, B16, TC1

. Investigating the role of tumor ER Stress response in the regulation of

anti-tumor immunity and UPR(Unfolded Protein response) in tumor cells.

. Analyzing data using BD FACS Calibur and Flow Jo Software.

. In vivo experiments and isolation of myeloid cells from mice.

Technical Skills:

Microbiology: Screening and Isolation of pure cultures of

microbes and their identification/staining methods,

Growing bacterial cultures under BSL-2

conditions

Molecular Biology: Isolation of genomic DNA, plasmids from bacteria,

PCR, Molecular markers-RAPD & RFLP,

Blotting- Southern and Western, SDS-PAGE,

Transformation, Transfection, DNA Sequencing,

Recombinant cloning techniques, mammalian

tissue culture,

Bioinformatics Tools: Sequence Analysis Tools; BLAST, FASTA, ORF

Finder, Vector NTI

Biochemistry: Enzyme assays, chromatography-TLC, quantitative

estimation of carbohydrates, proteins, nucleic acids,

HPLC, Biolayer Interferometry (Octet)

Equipment: Centrifuge, distillation column, chromatographic columns, Gas

chromatography chamber, spectrophotometer, autoclave,

oscilloscope and function generator, electrophoresis,

IR spectroscopy, crystallization, biomedical imaging

(MRI, EEG)

Programming Languages: Basic C, Matlab, LabVIEW

Operating Systems: Windows XP/Vista/7

Strong Interpersonal skills, excellent Oral and

Written Communication Skills

Patent and Publications:

. Invention Disclosure Report & Provisional Patent (Filed): Jody Berry,

Janean Fisher, Paul Waterman, Ella Mendoza, Mette Ejrnaes, and Priyal

Thakur

. "Design and characterization of a recombinant inhibitor of a Rabbit

Polyclonal Antibody which decreases background staining", BD Biosciences,

San Diego, 2013

. 12/8/2013-12/12/2013: IBC Antibody Engineering and Therapeutics

Conference, Huntington Beach, California

Presented a poster on "Design and characterization of a recombinant

inhibitor of a Rabbit Polyclonal Antibody which decreases background

staining" as a part of the Student Scholarship Program.

Work Experience:

7/2013 - 12/2013 Research and Development Intern, BD Biosciences, San

Diego:

. Design and Characterization of a Recombinant Inhibitor of a Rabbit

Polyclonal, which decreases background staining.

. Performed in-vitro mutagenesis, transformation, DNA sequencing, and

protein expression and purification procedures.

. Analyzed results using the BD FACS Canto II Flow Cytometer Octet Qke Bio

Forte System.

. Presented the summer research Poster at the Team Internship Program

conference.

. Significant contributions that led to product development of the

antibody.

1/2013 - 6/2013 Biomedical Intern, Nanocellect Biomedical Inc, San Diego:

Involved in human mammalian cell sorting using a highly integrated micro-fabricated

fluorescence activated cell sorter.

Analyzed data using signal processing in Matlab/Labview and carried out mammalian cell

culture techniques.

Developed and executed software test protocols for verification and validation.

7/2011 - 7/2012 Process Development Intern, Biocon Biopharmaceuticals,

Bangalore, India:

. Developed and completed a project on "Production of secondary

metabolites using microbial fermentation" that improved on existing

arthritis drug delivery procedure.

. Performed off-line analysis on seed and pilot Streptomyces and Pichia

fermentation, total nitrogen and phosphate count, HPLC to determine feed

and product concentration.

. Shake flask trials and media preparation, prepared seed culture to

determine optimum pH and temperature for production strain while

following GLP.

. Down streaming of Pichia broth after fermentation for the isolation of

trypsin, the purification steps involved microfiltration and

diafiltration while following cGMP.

. Conducted experiments using HPLC techniques to successfully identify,

quantify and purify the individual components of mixtures.

6/2009 - 9/2009 Project Intern, Ranbaxy Labs. Pvt. Ltd., & Sanofi Aventis, Hyderabad,

India:

Performed purification of proteins using chromatographic techniques such as HPLC, Ion

exchange chromatography and SDS PAGE electrophoresis gels. Also learned real time PCR

techniques and Southern Blotting.

Performed characterization of monoclonal antibodies using immunoassay technique such

as ELISA & Western Blotting.

Relevant Coursework:

- Bioelectronics - Biochemistry - Advanced Biomechanics

- Advanced Bio-photonics - Biomedical Imaging - Bio-nanotechnology

- Tissue Eng. and Regenerative Medicine - Thermodynamics - Graduate

Immunology

Activities:

7/2013- 4/2014 San Diego Chapter Correspondent, Oxbridge Biotech

Roundtable, San Diego Chapter

9/2012- 6/2013 Vice President of Publicity Affairs and Events, GradWISE

(Graduate Women in Science and Engineering)

9/2012- 7/2014 Bioengineering Representative, UC San Diego Graduate Student

Association

9/2012- 7/2014 Member of Bioengineering Graduate Society

9/2012- 7/2014 Jacobs Undergraduate Mentoring Program (Graduate Mentor)

REFERENCES:

Dr. John Watson: Professor, Department of Bioengineering, UC San Diego

Dr. Jose Morachis: President & CEO, Nanocellect Biomedical Inc, San Diego

Dr. Jody Berry: Director, Antibody Development, BD Biosciences, San Diego

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