Cell biology, immunochemistry, pharmacology

LARISSA V. FALCAO, M.S.

**** ******** ****** #**

Philadelphia, PA 19104

T: 267-***-****

E-mail: **************@*****.***

Drexel University College of Medicine (DUCOM), Philadelphia, PA

Education

Masters of Science in Pharmacology and Physiology, May 2014

Mumbai Educational Trust's Institute of Pharmacy (METIOP), Mumbai

University, MS

Bachelor of Pharmaceutical Sciences, July 2010

Skills Cell biology Instrumentation

Primary cell isolation and culture UV-visible absorption

Multi-well based cell proliferation, Florescence and confocal microscopy

migration and cytotoxicity assays Laser Capture Microdissection (LCM)

Cell lysis techniques Immunoflorescence

HPLC/GC

Biochemistry

Protein isolation and quantization Plate reading assays

Western blot analysis Computer

Graph Pad Prism 5

Animal Studies

Murine injections Image J

Murine necropsy Microsoft Office Suite

Tissue preparation Endnote

Cryopreservation SPSS statistical software

Cryosectioning

Drexel University College of Medicine

Research Experience

Graduate student, Department of Pharmacology and Physiology

Laboratory of Dr. Alessandro Fatatis (Aug 2012 – Jan 2013 & June 2013 – June

2014)

Histochemistry detection of disseminated tumor cells in the skeleton:

Collected and processed different tissues for cryosectioning, tumor cell

detection by fluorescence microscopy and immunohistochemistry

Performed western blotting assay for analysis of expression, activation and

signaling of different chemokine receptors in cancer cells

CX3CR1 receptor inhibition: Performed Dose-response studies on novel

small-molecule inhibitors of CX3CR1

CXCR4 and Androgen receptor inhibition: Performed cell viability assays to

study the survival of prostate cancer cells treated with antagonists of the

Androgen Receptor or the CXCR4 chemokine receptor, alone or in

combination.

Effect of CX3CR1 inhibitor on CXCR4 receptor: Studied the effect of a

CX3CR1 inhibitor on stimulation of CXCR4 receptors by SDF-1.

Trained and supervised junior students in various laboratory techniques.

Assisted in arranging, ordering and scheduling equipment use.

Laboratory of Dr. Paul Campbell (Jan 2013 – May 2013)

Performed cell proliferation & migration assays, cell culture and western

blotting to study the signaling of the Raf effector pathway downstream of K-

Ras in human pancreatic carcinoma.

METIOP, Mumbai University

Undergraduate Student, Pharmacology Laboratory (2009)

Dissected the rectus abdominus muscle from frog and the ileum from guinea

pig and recorded their contractions/relaxations when subjected to

Acetylcholine and Scopolamine with the help of a force transducer apparatus.

Additional Experience Drexel School of Education Drexel University, Philadelphia, PA

Conference Coordinator, March 2014

Center for Academic Success, Drexel University, Philadelphia, PA

Peer Academic Counselor and Tutor, June 2013 – May 2014

Cegedim Strategic Data, Mumbai, MS

Market Research Analyst, May 2012 – June 2012

Paradise Pharmacy, Nallasopara, Mumbai, MS

Registered Pharmacist, June 2011 – April 2012

Dispensed prescription drugs to patients and consulted them on the dosage, drug

interactions and adverse effects. Assisted in handling the purchase of merchandise.

Sanofi Aventis, Andheri (E), Mumbai, MS

Marketing Executive, Sep 2010 – March 2011

Mancare Pharmaceuticals Ltd., Vasai, Mumbai, MS

Summer Trainee, June 2009

Worked in the Quality Control and Quality Assurance department, operated the HPLC

and GC systems. Assisted in the production of Tablets, Capsules and Ointments.

Learned various aspects of the drug production cycle and packaging.

Discovery Day 2013. L. V. Falcao and P. M. Campbell: 'Are the pancreatic epithelial

Posters &

cells responsible for the dense fibrosis seen in pancreatic cancer?'. DUCOM, Fall

Presentations

2013

Current Topics Seminars in Pharmacology/Physiology Department. 'Stromal EGF and

IGF-I together modulate plasticity of disseminated triple-negative breast tumors'.

DUCOM, Spring 2014

Current Topics Seminars in Pharmacology/Physiology Department. 'Mammary Tumor

Formation and Metastasis Evoked by a HER2 Splice Variant'. DUCOM, Fall 2013

Current Topics Seminars in Pharmacology/Physiology Department. 'Androgen

Receptor Signaling in Circulating Tumor Cells as a Marker of Hormonally

Responsive Prostate Cancer'. DUCOM, Fall 2012

Sanofi Aventis Training Program. ‘Antibiotic Resistance’. Sanofi Aventis, Fall 2010

Sanofi Aventis Training Program. ‘ICU Infections’. Sanofi Aventis, Fall 2010

Seminar series. ‘Multiple Personality Disorder’. METIOP, 2009

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