Assistant Maintenance

ONIC

M SHAH

*** ***** ***** **********, ************* 01803 Cell: 309-***-**** Email: **********@*****.***

Profile

Highly motivated, MS degree accredited biological chemist with over 2 years of laboratory experience in research and

development of protein chemistry, bionanotechnology and microbiology seeking for Associate Scientist I positions

where I can contribute my skills and expertise to promote stability, growth and opportunity for advancement.

Laboratory Experience

• Aseptic bacterial culture (E. coli, P. aeruginosa, S. bovis, E. durans and S. epidermidis), bacterial enumeration

(CFU/mL), bacterial stock preparation and storage (Glycerol stock at -80 C), preparation of bacterial nutrient media

(Isosensitest, L.B and T.S broth), preparation of solid nutrient agar plates (L.B agar and T.S agar), bacterial growth

assays (microbroth dilution and spread plate assay), bacterial live/dead assay (XTT assay using 96-well microtiter

plate), antibacterial assays using antibiotics, bacterial membrane permeability assay using fluorescence microscope

using propoidium iodide dye

• Small scale and large scale expression of human growth factor (hFGF-1) protein in a cloned E. coli, extraction,

separation and purification of protein by lysis of bacterial cell using centrifuge, column chromatography, SDS-PAGE

and dialysis, characterization and protein-ligand interaction studies using chromatographic and bioanalytical

techniques (trpysin digestion, thermal denaturation and NMR)

• Sterile preparation of reagents, synthesis and characterization of gold nanoparticles

• Extraction of active components from plant extract using maceration and soxhlet apparatus

• Maintenance of biohazard, sterilization of equipments, glassware, reagents and biohazard waste

• Basic laboratory techniques involving pipetting, analytical balance, centrifuge, incubator, pH meter, lyophilizer

and autoclave

Instrumental/Computer Techniques

Spectroscopic analysis (UV Vis, FTIR and Fluorescence) •Proficient in data analysis using MS Excel, SOP

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Chromatographic techniques (SDS PAGE, Column, HPL writing and manuscript writing skills

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and GC) •Method development and instrument troubleshooting

•Material characterization using TEM, SEM, TGA, •Organization and maintenance of chemicals and

and DLS supplies

•Hands on experience with MS Office, Adobe

Acrobat and Adobe Photoshop

Accomplishments

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Monic Shah

Ogden College of Science and Engineering Outstanding Graduate Student Award, 2014

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Nell Skean Outstanding Laboratory Assistant Award, 2014

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Best Graduate Oral Presentation Award, 44th WKU REACH Conference, 2014

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Carl. P. McNally Graduate Chemistry Fellowship Award, 2013

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Three peer reviewed publications with more than 15 conference presentations

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Kentucky Innovation Network Entrepreneur Award, 2014

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$1500 Research grant from Graduate School, WKU

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Student Sponsorship to attend 23rd NSF EPSCoR Conference at Nashville, KY

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OVTAS Sponsorship to attend 41st NATAS Conference in Bowling Green, KY

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$100 for Best Graduate Student Concept during Business Plan Competition

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Education

Master of Science, Chemistry August 2014

Western Kentucky University, Bowling Green, Kentucky

Dissertation: “Antimicrobial Nanoparticles: A Green and Novel Approach to Enhance Bactericidal Activity of Commercial

Antibiotics”; Adviser: Dr. Rajalingam Dakshinamurthy

• 4.0 GPA; BPMCT certified Graduate Student Instructor

• Graduate Instructor Intro to Chemistry

• Teaching Assistant for General Chemistry lab and Biochemistry lab

• Chemistry tutor: 4 hours of weekly tutoring in WKU

Research Experience

Research Assistant Sep 2012 to May 2014

Western Kentucky University, Bowling Green, Kentucky

•Assisted in planning and method development for several projects involving antibiotic gold nanoparticles

•Collected necessary literature and established new protocols for the material analysis

•Performed synthesis and analytical characterization of nanoantibiotic formulation

•Measured, analyzed and recorded the experimental data and presented in weekly reports and lab group meetings.

•Directed multiple projects simultaneously in coordination with several undergraduate students

•Generated SOP’s for various experiments involved in the projects

•Presented the research data in various scientific conferences; Proof read grants and manuscripts

Publications (3 more in drafting stages)

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Monic Shah

• M. Shah, V.D. Badwaik and R. Dakshinamurthy, “Various Methods of Synthesis of Gold Nanoparticles and their

Antibacterial Applications”, Fron. Biosci. 19:1320 44 (2014)

• M. Shah, V.D. Badwaik and R. Dakshinamurthy, “Biological Applications of Gold Nanoparticles”, J. Nanosci.

Nanotechnol. 14, 344 362 (2014)

• V.D. Badwaik, C.B. Willis, D.S. Pender, R. Paripelly, M. Shah, Y.A. Kherde, L.M. Vangala, M.S. Gonzalez and R.

Dakshinamurthy, “Antibacterial Gold Nanoparticles Biomass Assisted Synthesis and Characterization”, J.

Biomed. Nanotechnol. 9, 1719 (2013)

• M. Shah, Y.R. Behara and B. Jagadeesh, “Phytochemical Screening and In Vitro Antioxidant Activity of Aqueous and

Hydroalcoholic Extract of Bacopa Monnieri Linn.”, Int. J. Pharm Sci. Res. 3(9), 3418 (2012)

• M. Shah, P.K. Reddy, Y.R. Behara and C.Mouli, “Anti diarrhoeal Activity of Toddalia asiatica (L.) in Castor Oil Induced

Diarrhoea in Rats”, Int. J. Res. Pharmacol. Pharmacotherapeu. 1(1), 5 9 (2012)

• Ch. Madhu, J. Swapna, K. Neelima and M.V. Shah, “A Comparative Evaluation of the Antioxidant Activity of Some

Medicinal Plants Popularly Used in India”, As ia n J. Re s . Pharm. Sci. 2(3), 98 (2012)

Affiliations

American Chemical Society (ACS), Student Member

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American Physical Society (APS), Student Member

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Kentucky Academy of Sciences (KAS), Student Member

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Indian Pharmaceutical Association (IPA), Student Member

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International Pharmaceutical Federation (FIP), Student Member

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Member of Chem Club and Indian Student Association (ISA) at WKU

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References

Available on request

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Monic Shah

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