Assistant University

ANNA SBERNA

*** ******** *** • New Hope • PA ****8 • *********@*****.*** • 215-***-****

PROFILE:

A molecular biologist with experience in a variety of laboratory techniques that is able to apply

creativity to problem solving in scientific experiments. Does well in environments that require

strong communication and critical thinking skills. Interested in advancements in science and

medicine that provide insight into disease pathologies and potential novel treatment options.

EDUCATION:

The Pennsylvania State University, University Park University Park, PA

Masters of Science, Genetics May 2014

West Chester University of Pennsylvania West Chester, PA

Bachelor of Science, Cell and Molecular Biology May

2012

RESEARCH EXPERIENCE:

The Pennsylvania State University, University Park University Park, PA

Graduate Research Assistant; Genetics Aug 2012-May 2014

• Cloned and verified the full sequence of a novel EAG homolog in the Ctenophore

Mnemiopsis leidyi.

• Designed primers for the cloning of this gene using the partially sequence obtained from

the Mnemiopsis Genome Project website. Created primers in areas where translated

sequence showed homology to the major protein domains observed in EAG channels.

• Characterized the heteromeric Kv2.1/6.4 knockout mouse model using a variety of

behavioral assays to look for motor deficits, anxiety, and seizure susceptibility.

• Oversaw maintenance and breeding of Kv6.4-/- mouse line, including the breeding of a

ZP3 Cre Kv6.4-/+ mouse.

• Contributed to experiments to determine stoichiometry of Kv2.1/6.4 channel.

• Tagged Kv2.1 and Kv6.4 cDNAs with both CFP and EYFP proteins. Subsequently used

both tagged and untagged cDNAs to make RNA to inject into Xenopus oocytes, the

membranes of which could be observed using TIRF microscopy.

Laboratory Teaching Assistant

• Instructed two classes of more than 20 students on a weekly basis.

• Conducted lessons focusing on experimental molecular biology including DNA and RNA

isolation, qRT-PCR, and data analysis.

• Emphasized the importance of the proper handling of lab equipment and sterile

technique.

The West Chester University of Pennsylvania West Chester, PA

Independent Study May 2011-Jun 2011

• Aided in research regarding the expression of alcohol dehydrogenase (ADH) isozymes in

prostatic epithelium and its relationship to the pathogenesis of prostate cancer.

• Created RNAs from human cDNA samples and used qRT-PCR to quantify amounts of

ADH isozymes in human prostate epithelium samples.

TECHNICAL SKILLS:

Molecular Biology: DNA cloning; primer design using partial gene sequence; DNA/RNA

isolation; in vitro synthesis RNA, PCR and qRT-PCR; optimization of conditions for cloning and

genotyping PCRs; bacterial transformation and culture; transfection of mammalian cells

Protein Chemistry: SDS-PAGE

Immunoblotting: Western blotting

Animal Handling (mouse): Conducting and analyzing behavior assays including open field,

elevated plus maze, gait test, and seizure threshold; organization and management of mouse

breeding lines; IP drug administration

Electrophysiology: Injection of RNAs into Xenopus oocytes and some experience with taking

electrophysiological recordings.

General: Proficient with Microsoft Word, PowerPoint, Excel, Lasergene (versions 8 and 11)

software, and Noldus behavior analysis software.

PRESENTATIONS:

“Understanding the role of the Kv2.1/6.4 heteromer in vivo.” Talk. Center for Molecular

Investigation of Neurological Disorders (The Pennsylvania State University), Oct. 18, 2013.

“Characterization of the Kv2.1/6.4 mouse model.” Poster presentation. Center for Molecular

Investigation of Neurological Disorders Retreat (The Pennsylvania State University), Sept.

28, 2013.

“Revealing cellular secrets: the link between alcohol and prostate cancer.” Talk. Presented to the

Board of Trustees (West Chester University), July 21, 2011.

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