Engineer Plant

Yonghua Luo

*** ******* ***, *** **

Jersey City, NJ, 07306

E-Mail: *******@***.*******.***

Tel: 720-***-****

SUMMARY

Highly competent microbiologist and biochemist with over 10 years of

experience in microbiology and biochemistry field; Capable of solving

complicated problems based on intuitive skill and extensive experience

derived from many cross-disciplinary projects; Ability to work with people

with diverse interests as well as work independently; Skilled researcher

with over 10 journal articles published in international journals.

EMPLOYMENT

Sep.2013-Present: Postdoctoral Associate, Weill Cornell Medical College,

New York City:

1) Developed a new high-throughput native immunoglobulin heavy-chain

and light-chain pairing method

(2) Engineering chimeric/humanized antibody

(3) Generating genetically modified T cells against tumor

cells by single-chain variable fragment (scFv) with lentivirus

system

Aug.2010-Sep.2013: Postdoctoral Researcher, University of Texas at Austin,

Austin, Texas

Achievements:

(1) Establishment of baculovirus expression system.

(2) Crystallization and optimization of a newly reported CTD

(C terminal domain of RNA

polymerase II) phosphatase-Rtr1.

(3) Deciphering of Drosophila melanogaster symplekin-

Ssu72,symplekin-Ssu72-CTD complex structures.

(4) A newly found fine-tuned modulation of Ser-5(P)

dephosphorylation of canonical CTD repeats by protein kinetics

method.

Aug. 2007 - Aug. 2010: Postdoctoral Researcher, National Renewable Energy

Laboratory, Golden, Colorado

Achievements:

1) Genetically engineer molecular probes (fluorescently labeled CBM

proteins) to map the

distribution of carbohydrates in biomass.

2) Assembly of mini-cellulosome serving as a model to engineer

cellulosome with high

cellulose-degrading efficiency

(3) Classification and establishment of multi-functional

cellulolytic enzyme system.

(4) Three dimensional structure determination of CBM4 protein

from Clostridium thermocellum

cellulase K

.

Jan.2004-Aug.2007: Graduate student, The Pennsylvania State University.

Achievements:

1) Evaluation of the effect of glucose concentration on anaerobic

microbial community composition in continuous flow bioreactor for

biohydrogen production

2) Evaluation of the effect of Oxidation-Reduction potential on

anaerobic microbial community composition in continuous flow

bioreactor and batch experiments for biohydrogen production

3) Isolation and characterization of dominant hydrogen producer from

a mixed soil inoculum

July.1997 - Jan.2004: Research Assistant, Guangdong Institute of

Microbiology,

Guangzhou, Guangdong, P.R.China

Achievements:

1) The treatment of waste water by biological methods.

2) Development of a biological deodorizer.

EDUCATION

1) Aug.2007: Ph D in Environmental Microbiology. Department of Crop and

Soil Sciences. The Pennsylvania

State University.

2) July.1997: M.S., Microbiology, Wuhan Institute of Virology, Chinese

Academy of Sciences

PROFESSIONAL SKILLS

Microbiology

1) Microbial screening and cultivation (aerobic and anaerobic microbes).

2) Fermentation

3) Yeast genetics (Complementation analysis, Two hybrid, Gene loop forming

analysis etc)

4) Construction of phylogenetic tree based on 16S rRNA sequences (Neighbor-

Joining Parsimony Maximum Likelihood)

5) Fluorescent in-situ hybridization (FISH)

6) Microbial expression systems (bacteria, yeast and baculovirus) for

protein production.

(Bacteria expression system: E.coli Yeast expression system: Pichia)

Biochemistry

(1) SDS-PAGE

(2) Western blotting.

(3) Differential scanning fluorimetry (DSF)

(4) Construction of phylogenetic tree based on protein sequences (Neighbor-

Joining Parsimony Maximum Likelihood)

(5) Protein kinetics.

(6) Antibody/Protein engineering

(7) Protein purification by FPLC (affinity, ion exchange, hydrophobic

interaction and gel filtration), dynabeads etc.

(8) Crystallization of proteins: sitting drop, hanging drop, microseeding,

macroseeding, substrate soaking and cocrystallization.

(9) Protein structure modeling

(10) Deciphering of protein three dimensional structures (HKL2000, CCP4,

Phenix, Autobuster and COOT softwares): solving the phase problem with

molecular replacement, SAD and MAD methods. Refinement of collection data

.

(11) Enrich experience of protein expression from a variety of host cells

(bacteria, yeast, baculovirus and

mammalian cells) for protein production.

(Bacteria expression system: E.coli Yeast expression system: Pichia

Baculovirus expression systems: Bac-to-Bac, BacPAK Mammlian cells: CHO

and HEK293)

Cell

(1) Insect cell cultivation

(2) Mammalian cell cultivation

(3) Isolation of lymphocytes from blood and immortalization of human

lymphocytes by Epstein-Barr virus

(4) Yeast genetics (Complementation analysis, Two hybrid analysis etc)

(5) Fluorescence Assisted Cell Sorting (FACS)

(6) Cell expression systems (Insect and mammalian cells) for protein

production.

(Insect cell expression systems: Bac-to-Bac, BacPAK Mammalian cell

expression systems: CHO, HEK293)

7) Transfection, transduction

Molecular biology

1) Construction of all kinds of vectors

2) DNA fingerprinting

3) Real-time PCR

4) RNA extraction, Reverse transcription, RNA purification by resin or

dynabead, RNA synthesis by in vitro transcription.

5) Reverse transcription PCR, Bridge PCR, Emulsion PCR, Smarter RACE PCR

etc.

6) DNA cloning with a variety of methods (digestion-ligation, ligation

independent and recombinant cloning etc)

7) A variety of site-directed mutation methods

8) cDNA library construction

9) Southern and northern blotting

(10) Yeast surface display

REPRESENTIVE PUBLICATIONS

(1) Luo Y, Wenzong Li, Anne S. Tibbetts, Christopher Yellman, Mengmeng

Zhang, Dean Appling and Yan

Zhang.2014. Conservation of Ssu72-template protein interface for

eukaryotic transcription 3'-end processing

and termination. (Manuscript)

(2) Luo Y, Yogesha S.D., Cannon J, Yan W, Brodbelt J, Zhang Y. 2013. Novel

Modification on C-terminal

Domain of RNA Polymerase II can Fine-tune the Phosphatase Activity of

Ssu72. ACS Chemical Biology.

(3) Zhang, M., Wang, X.J., Chen, X., Bowman, M.E., Luo, Y., Noel, J.P.,

Ellington, A.D., Etzkorn, F.A. and

Zhang, Y. 2012. Structural and kinetic analysis of the prolyl

isomerization/phosphorylation cross-talk in the

CTD code. ACS Chemical Biology

(4) Wei H, Tucker M, Baker J, Harris M, Luo Y, Xu Q, Himmel M, Ding

SY.2012.Tracking dynamics of plant

biomass composting by changes in substrate structure, microbial

community, and enzyme activity.

Biotechnol Biofuels. 2012. 10;5(1):20

(5) Alahuhta M, Luo Y, Ding S-Y, Himmel ME and Lunin VV, 2011, Structure of

CBM4 from Clostridium

thermocellum cellulase K, Acta Crystallographica Section F Structural

Biology And Crystallization

Communications. F67:527-530.

(6) Xu Q, Luo Y, Ding S-Y., Himmel M.E., Bu L, Lamed R and Bayer EA. 2011.

Multifunctional Enzyme

systems for plant cell wall degradation. Comprehensive Biotechnology

(2nd Ed).

(7) Dagel DJ, Liu Y-S. Zhong L, Luo Y, Himmel ME, Zeng Y, Ding S-Y and

Smith S. 2011. In situ imaging of

single carbohydrate-binding modules on cellulose microfibrils.

Journal of Physical Chemistry B 115, 635-

641.

(8) Dagel DJ, Liu Y-S. Zhong L, Luo Y, Zeng Y, Himmel ME, Ding S-Y and

Smith S. 2011. DOPI and PALM

imaging of single carbohydrate binding modules bound to cellulose

nanocrystals. Proc.SPIE. 7905:

(9) Himmel M.E., Xu Q, Luo Y.H, Ding S.Y., Lamed R and Bayer E. 2010.

Microbial enzyme systems for

biomass conversion: Emerging Paradigms. Biofuel. 1(2):323-341.

(10) Liu Y.S., Luo Y, Backer J.O., Zeng Y,, Himmel M.E.,Smith S, Ding S.Y.

2010. A single molecule study

of cellulose hydrolysis of crystalline cellulose. Proc.SPIE. 7571:

757103.

(11) Liu Y.S., Zeng Y, Luo Y, Xu Q, Himmel M.E., Smith S.J. and Ding S.Y.

2009. Does the cellulose-binding module move on the cellulose surface?

Cellulose. 16:587-589

(12) Luo Y, Zhang H, Salerno M, Logan B.E. and Bruns M.A. 2008. Organic

loading rates affect composition of soil-derived bacterial communities

during continuous, fermentative biohydrogen production. International

Journal of Hydrogen Energy. 33(22): 6566-6576.

(13) Luo Y.H, Fang X.P., Cao W, Zong X, Sun G. 2004. The Assessment of A

Deodorizer's Effect on the

Removal of Malodor Generating from the Process of Garbage

Collection. Journal of Microbiology.

24(5):103-105. (In Chinese)

(14) Luo Y.H., Deng S.E., Sun G.P.. 2003. The odor-removal experiments

conducted with a

Microbial deodorizer. Environment and Urban Ecology. 16(3):23-25.

(In Chinese)

(15) Fang,X.P., Luo Y.H., Deng S.E, Ceng Y.H., and Sun G.P. 2003. Research

on deodorization of

municipal solid wastes by a biofilter. Environment and Urban

Ecology.16(1):34-36. (In Chinese)

(16) Zeng,G.Q., Xu M.Y, Luo Y.H., Cai W.J. and Sun G.P.. 2002. Application

of trickling

filters in treatment of wastewater from gas-generating with heavy

oil. Microbiology.

29:52-54. (In Chinese)

(17) Luo,Y.H. and Guo J. 2002. Molecular detection of homology of hydrogen-

uptaking gene

in Azospirrium brasilense. Journal of Microbiology.22:11-13. (In

Chinese)

(18) Luo,Y.H., DENG S., Li, T.H., Sun G.P., Feng Y.Q. and Guo, J. 2002.

Kinds, Control, and Identif

ication of Microbes in Pollutant in an Ethylene Plant Circulating

Water. Journal of Microbiology.

22: 36-39. (In Chinese)

(19) Wei,H., Luo Y.H. and Guo J et al. 1999. Technology studies on

production of

biological-organic-inorganic compound fertilizer by utilizing manure

of livestock

and poultry.Biotechnology.9:30-33 (In Chinese)

CONFERENCE PPRESENTATIONS

1) Luo Y, Tibbetts A, Yugesha S, Ying W, Zhang Y.2012. Crystal structure of

Drosophila melanogaster symplekin-Ssu72-CTD complex. Texas Enzyme

Mechanisms Conference.

2) Yan J Zhang, Mengmeng Zhang, Yonghua Luo, Yong

Zhang.2011.Dephosphorylation Regulation of RNA polymerase II in

Eukaryotic Transcription.PDB40 Symposium.

3) Liu Y.S, Luo Y,Baker J.O., Zeng Y, Himmel M.E., Smith S, and Ding S.Y.

2010. Studying cellulase hydrolysis of cellulose with AFM and TIRF-M.

32nd Symposium on Biotechnology for Fuels and Chemicals.

(4) Edward A Bayer, Qi Xu, Yonghua Luo, Shi-You Ding, Raphael Lamed,

Michael E Himmel. 2010.

Microbial enzyme systems for biomass conversion: Emerging paradigms.

32nd Symposium on

Biotechnology for Fuels and Chemicals

(5) Luo Y, Xu Q, Liu Y.S., Zeng Y, Himmel M.E., and Ding S.Y., 2009, Using

carbohydrate-binding

module as molecular probe to map biomass polysaccharides.31st

Symposium on Biotechnology for

Fuels and Chemicals.

(6) Luo Y and Bruns M.A. 2007. The effect of L-cysteine on composition of

biohydrogen-producing

bacteria communities in batch cultures. CrossOver 2007 Bioenergy:

From Fields to Wheels.

(7) Luo Y, Logan B.E. and Bruns M.A. 2006. The effect of redox potential on

hydrogen by

Clostridium sp. Strain LYH1 isolated from Soil. The 9th Annual

Environmental Chemistry Student

Symposium.

(8) Luo Y, Zhang H, Michael S, Logan B.E., Bruns M.A. 2004. Isolation of

dominant hydrogen-producers from

continuously fed bioreactors. 2004 Hydrogen Day

PATENTS

(1) Microbe deodorant. Luo Yonghua, Sun Guoping. (2004) (Commercialized)

(China) CN1502372

(2) Biologic deodorization method and its device. Sun Guoping, Fang

Xiangping, Luo Yonghua. (2003) (Commercialized) (China) CN1445013

(3) Microbial deodorant and its preparation method. Sun Guoping, Luo

Yonghua, Deng Suier.

(2003) (China) CN1411872

AWARDS

(1) 2011 Year Guangdong Patent Excellence Award Certificate (Biological

deodorization method and

its device) (China)

(2) 2003 Year State Environmental Protection Certificate for Scientific

and Technological

Achievements (Microbe Deodorant) (China)

(3) 2000 Year State Environmental Protection Certificate for Scientific

and Technological

Achievements (The Pilot Scale Test of the Treatment of Wastewater

Containing High

Concentrations of Ammonia Nitrogen and Refractory Organics with a New

Microbial Method).

(China)

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