Quality Assurance Control

Irvine, California ************@*****.*** 858-***-****

Lin Feng

Research professional with 5 years of academic research experience and 2 years of industry experience.

Hands-on experience in manual patch clamp, animal models (rodent) and surgeries,

immunohistochemistry, PCR, ELISA, western blotting, confocal microscopy and data analysis.

Expertise in ion channel biophysics/pharmacology (electrophysiology), pharmacology, histology,

molecular biology and neuroscience with strong understanding in Good Manufacturing Practices

(GMPs), Good Laboratory Practices (GLPs) and clinical trials. Author of four articles in peer reviewed

journals and proficiently in writing protocols, manuscripts and poster presentations. Excellent

interpersonal communication skill as well as attention to detail, solid problem solving ability.

EXPERIENCE

Research Assistant

Southern Illinois University School of Medicine - August 2009 to May 2014

Investigated (1) molecular and cellular mechanisms of neuropathic pain and autonomic disorders; (2)

synaptic physiology and ligand-/voltage-gated ion channel pharmacology in relation to cognitive and

autonomic disorders; (3) development of drug intervention approaches for cerebral ischemic stroke and

traumatic brain injury using positive allosteric modulation (PAM).

Established and maintained electrophysiology apparatus

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Mentored two Ph.D. students to conduct slice electrophysiology experiments; mentored two

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interns to conduct tissue sectioning (paraffin and frozen) and immnunohistochemistry

experiments

Presented posters at Society for Neuroscience meetings and annual Trainee Research Symposium

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at Southern Illinois University School of Medicine

Contributed >40 reconstructed neuron images to NeuroMorpho website

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Constructed and analyzed hundreds of ex vivo 3D confocal fluorescent images of the traced

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neurons in the Neurolucida system

Implemented in vivo rodent stereotaxic pressure injections of retrograde fluorescent tracers into

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four nuclei separately to label neurons in the nucleus of solitary tract

Generated rodent diabetic models, analgesia models; tracked, analyzed and interpreted the

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rodents’ behavior of pain testing using statistical software (SPSS)

Performed hundreds of immunohistochemistry, PCR, western blotting experiments to examine

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the expression of nicotinic acetylcholine receptors (nAChRs), TRPV1 and TRPA1

Developed acute slices of hippocampus, nucleus of solitary tract, dorsal motor nucleus of the

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vagus, paraventricular nucleus of hypothalamus

Investigated ion channel biophysics and ion channel pharmacology of functional nAChRs,

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TRPV1, TRPA1

Organized “Brain Awareness Week” activity among 7th and 8th grade students in Franklin

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Middle School in Springfield to promote brain science and research.

Published three first-author papers and one second author paper in high-rank, peer-reviewed

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journals

1. Feng L. and Uteshev V.V. Project target-specific action of nicotine in the caudal nucleus

of the solitary tract. J. Neurosci. Res. (2014) (In press)

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2. Feng L., Sametsky, E., Gusev A.G. and Uteshev V.V. Responsiveness to nicotine of

neurons of the caudal nucleus of the solitary tract correlates with the neuronal projection

target. J. Neurophys. (2012) 108: 1884-94 (PMCID: PMC3544998).

3. Kalappa B.I., Feng L., Kem W.R., Gusev A.G., and Uteshev V.V. Mechanisms of

facilitation of synaptic glutamate release by nicotinic agonists in the nucleus of the solitary

tract. Am.J.Physiol. Cell Physiol. (2011) 301(2): C347-61 (PMCID:PMC3154558).

4. Feng L, Uteshev VV, Premkumar LS. Expression and Function of Transient Receptor

Potential Ankyrin 1 in the Nucleus Tractus Solitarius. (Submitted)

International Trade Specialist

Wuhan Grand Pharmaceutical Co., Ltd. - May 2007 to May 2009

• Achieved sales goals of pharmaceuticals and medical products by 109%

• Negotiated with clients and collaborated with Quality Control and Quality Assurance managers

for timely deliveries and quality related issues

SKILLS

In vivo techniques: laboratory rodent care, drug administration (PO, IP, IV, IM), small animal

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(rodent) stereotaxic surgeries, anesthesia, blood and tissue samples collection, in vivo focal drug

injections, in vivo fluorescent tracing, brain anatomy, behavioral models

Histology: tissue embedding (paraffin & frozen) and sectioning, immunohistochemistry,

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immunofluorescence

Imaging/Microscopy: in vitro and ex vivo 3D confocal fluorescent imaging (Neurolucida

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system and Stereo Investigator) microscopy

Electrophysiology: patch-clamp electrophysiology in acute brain slices and dissociated cells

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Molecular Biology: cell culture, tissue DNA & RNA preparation, qPCR, RT-PCR, SDS-PAGE,

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ELISA, western blot, cell-based assays, calcium imaging

Data Analysis: behavioral, electrophysiological (Axon pClamp), morphological (Neurolucida),

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Statistical (SAS, SPSS)

Computer: Microsoft Office, micro database, language C, Origin, Coreldraw, EndNote

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Planning and Management: materials ordering, sample database management, implementation

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and enforcement of laboratory Standard Operating Procedures (SOPs), protocol formulation and

method optimization

EDUCATION

PhD of Pharmacology and Neuroscience

Southern Illinois University at Carbondale - August 2009 to May 2014 - GPA: 3.5/4.0

Dissertation: Modulation of synaptic transmission in the caudal nucleus tractus solitarii (cNTS) by

nicotinic acetylcholine receptors (nAChRs) and transient receptor potential (TRP) channels.

BS of Pharmacy Administration

Shenyang Pharmaceutical University, China - August 2003 to May 2007 - GPA: 3.62/4.0

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