Assistant Plant
Resume:
Jared W. Bailey
Lexington KY, **517 USA
*****.******@****.***
SUMMARY
A molecular biologist with more than 4 years of industrial experience in
the isolation and cloning of biosynthetic genes, and in the development of
bacterial and yeast strains for the full scale commercialization of
specialty chemicals used in the flavors and fragrance industry. Some
specific accomplishments include the development of a yeast strain for the
commercial scale production of valencene and the isolation of a gene for
the production of sclareol. In addition to these accomplishments, there
exists a proven track record of both meeting and exceeding company goals
along with mentoring junior scientists.
EXPERIENCE
Research Department, Allylix Inc., Lexington, KY Sept 2008-
June 2014
Molecular Genetics:
My primary responsibilities included the characterization of novel plant
proteins for the production of terpenoids, mutagenesis of these proteins
to allow for a change in production/product profile, and development of
the S. cerevisiae strains necessary for commercially viable
manufacturing. Some highlights of these goals include:
. Isolated and cloned sclareol synthase using RACE PCR and MiSeq from a
previously unidentified plant source (patent pending)
. Worked on production strain development by creating auxotrophic
mutants, testing promoter optimization for differential expression of
protein, and by genomically integrating pathways using REMI and the Ty
transposable element methods
. Optimized enzyme (valencene synthase) specificity and turnover using
directed and saturation mutagenesis which, along with the above, lead
to a production strain that became the benchmark production strain for
two years
. Created dual plasmid expression of the 2-C-methyl-D-erythritol 4-
phosphate (MEP) in yeast
. Constructed polyhistidine tagged expression plasmids, used a Biorad
Profinia for protein purification, and then performed in-vitro
substrate specificity/kinetic analysis using GC-MS for turnover
quantitation
. Checked plasmid retention and transcription by using both qPCR and RT-
PCR and increased retention through the use of a defective promoter
driven auxotrophic marker
. Developed high-throughput assays for the extraction, separation, and
quantification of terpene products from two-phase fermentation broth
using GC-FID and LC-MS-MS
. Implemented an interdepartmental purchase of bioinformatics software
that allowed for increased workflow through uniform data storage
. Used different types of plasmid construction including Gibson
assembly, Topo cloning, traditional ligation, and yeast homologous
recombination
. Expertise in the use of several bioinformatics software packages
(Snapgene, Geneious, Pymole, and ApE)
. Supervised and trained interns and other assistants
. Maintained a managerial reviewed lab notebook
. Presented data monthly in companywide meetings
. Created and maintained a plasmid and strain library in frozen glycerol
stocks
. Proficient in CTC PAL robotics programming.
. Performed general maintenance on numerous laboratory equipment
including GC-FID, UPLC-MS, centrifuges, shakers, and deep freezers
Fermentation and Process Development
My primary responsibility was to develop fermentation processes for the
commercial scale production of sesquiterpenes.
. Performed fermentation experiments using 2 and 10 liter New Brunswick
Bioflo 110 fermenters under the control of Biocommand
. Implemented HPLC and GC-FID detection of important, previously
untraced metabolites
. Developed technical experience with the BMG Omega plate reader, the
YSI 2700 Analyzer, and different UV-spectrophotometers
. Put into place a maintenance system that decreased overall
contamination and equipment down time
. Actively participated in the technology transfer of fermentation
processes to contract manufacturers.
. Developed digital record keeping system that allowed researchers to
monitor their strains on a day to day basis
. Researched labor and cost saving alternative methodology and equipment
Biology Department, Otterbein University, Westerville, Ohio June 2005
- May 2008
Teachers Assistant
. Assisted students with identifying bacteria based on morphology
. Lead test preparation session and lesson reviews
. Taught proper lab procedures including aseptic techniques and safety
. Used and taught light field microscopy
Research Assistant
. Designed and completed independent research on the impact of the fungal
load of the native back beetle, Ips pini
. Collected the beetle species and identified parts of the fungal load
through morphological methods including microscopy and carbohydrate
utilization
Laboratory Assistant
. Assisted laboratory director with laboratory preparation and teardown,
including autoclaving waste, pouring media, and general sterilization
techniques
SKILLS
PCR; qPCR, RT-PCR; REMI; primer design; DNA purification; de-novo
sequence assembly; plant/yeast RNA extraction; cDNA library
construction; restriction digest; agarose gel purification;
polyacrylamide gel analysis; Western blot; protein purification; S.
cerevisiae and E. coli transformation; high-throughput screening of
yeast cultures; aseptic technique; gene knockout/genomic integration;
metabolite analysis; analytical method development; maintenance of GC-
MS/LC-MS-MS/GC-FID/HPLC/YSI; frozen stock and plasmid database
organization; laboratory presentations; literature research; technology
transfer; lab safety
EDUCATION
Master's Degree in Medicinal Sciences August 2011-Present
Integrated Biomedical Sciences
University of Kentucky, Lexington, Kentucky
Bachelor of Science in Life Science December 2008
Concentration: Molecular Biology
Otterbein University, Westerville, Ohio
Recipient, 2007 E. Jeanne Willis from Department and of Life and Earth
Science
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