BANABIHARI GIRI, PH.D.
Pfafftown, NC-27040,
Phone: 336-***-****
Email: **************@*****.***
Citizenship: Permanent Resident of USA (Green Card)
Objective: A position as an analytical chemist taking advantage of my
knowledge on chemistry and biochemistry for detection and qantification of
chemical and biochemical molecules.
Highlights:
. Broad knowledge in the synthesis, purification and characterization of
biomolecules
. Experienced in Inductively Coupled Plasma Optical Emission Spectroscopy
(ICP-OES), HPLC, Spectrophotometer and Spectrofluorimeter.
. Skilled in identifying and preparing novel biomaterial for using rapid
screening of gene for diagnostic use
. Proficient in preparing nanomaterial having high efficiency and
extended photostability
. Proven track record as a team leader with excellent interpersonal and
communication skills
Education:
Ph.D. Biochemistry, University of Calcutta, India
2000
. Mentor: Birendra B. Biswas
. Research Thesis: "Phosphoinositdes signaling and calcium homeostasis in
Entamoeba histolytica"
M.S.; Department of Biochemistry, University of Calcutta, India.
1991
B.S.; Department of Chemistry, Utkal University, India.
1988
Experience:
Research Scientist
2013-Present
Research Focus:
. Working on structural and functional study of nucleic acids employing
both biochemical and analytical chemistry approaches.
. Apart from own research project, I am responsible for running
sophisticated Instruments core facility of the Institute.
. Oversee the day to day operation of the facility
. Devise and implement strategies for outreach and education. These
include participating in Research Facilities Fairs, organizing
workshops/seminars
. Interact with users to discuss experimental needs, technical issues and
fulfill the mission of the facility by providing expeditious, effective
technical support to the users
Postdoctoral Research Associate, Furman University, Greenville, SC.
2012-2013
. Mentor: Jeffrey T. Petty
. Research Focus: Synthesis and characterization of DNA silver
nanocluster for biolabelling
. Designed, synthesized and characterized silver nanocluster using
cytosine rich DNA oligonucleotides
. Target specific gene by exploiting DNA-Ag-nanocluster near infrared
emission properties
Research Fellow, Wake Forest University Health Sciences, Winston Salem, NC
2005-2012
. Mentor: James P. Vaughn
. Research Focus: Synthesis and characterization of quadruplex DNA for
regulation of cancer gene expression
. Designed, synthesized and characterized of quadruplex DNA structure in
vitro
. Resolved quadruplex DNA structure by enzyme G4R1
Research Fellow, National Institute of Health, Baltimore, MD
2002-2005
. Mentor: Dennis D Taub
. Research Focus: Study on chemokine signaling
. Prepared lipid cluster from human T cell, which act as a platform for
cell signaling
. Identified varieties of protein involved in cells signaling and
characterized them based on isoelectric focusing and mass spectrometry
Postdoctoral Fellow, Thomas Jefferson University, Philadelphia, PA
2001-2002
. Mentor: Philip Tsichlis
. Research Focus: Cloning, sub-cloning and expression of cancer gene
protein kinase B (AKT)
. Cloned gene(s) for generation of transgenic mice
. Explored the underlying mechanism for thymopoesis
Postdoctoral Fellow, Wake Forest University Health Sciences, Winston
Salem, NC 1998-2001
. Mentor: Gary M. Kammer
. Research Focus: Isolation and purification of protein kinase A (PKA)
from lupus patient blood
. Isolated and purified human T cell from SLE patient blood and
quantified the expression of above gene by designing competitive
polymerase chain reaction (cPCR)
Awards and Certification:
. Second Best Poster presentation at Wake Forest University Cancer
Biology Retreat, 2011
Winston Salem, NC
. National Institute on Aging Director's "Thank You" Award for
extraordinary service and 2006
team work
. International travel award from federation of clinical and
immunological society 2004
Affiliations:
. American Association for the Advancement of Science
2004
. American Association of Immunologists
2004
Publications (16) and Presentations (17):
Selected publications:
. Jeff T. Petty, Orlin Sergev, David Nicholson, PM Goodwin, Banabihari
Giri, DR. McMullan. A silver Cluster-DNA Equilibrium. Anal.Chem 2013 Oct
15;85(20):9868-76
. Banabihari Giri; Jeff Petty; Ian Miller; David Nicholson; Orlin Sergev;
Sandra Story. Silver Clusters as both Chromophoric Reporters and DNA
Ligands. (2013), Anal.Chem. 85, 2183-2190
. Banabihari Giri; Philip J. Smaldino; Ryan Thys; Steven D. Creacy; Eric
Routh; Simon Lattmann; Yoshikuni Nagamine; Jim Vaughn and Steven Akman.
G4 resolvase-1 tightly and specifically binds and unwinds unimolecular G4-
DNA (Nucleic Acid Research, 2011 Sep 1; 39(16):7161-78
. Banabihari Giri, Philip J. Smaldino, Eric Routh, James Vaughn, Steven
Akman. G4R1/RHAU Interactional with c-Myc G4-DNA Quadruplex. Wake Forest
University Cancer Biology Retreat, Winston Salem, NC, August 4-5, 2011.
(Second Prize awarded)
. Banabihari Giri, Viswa Deep Dixit, Manik Ghosh, Gary Collins, Islam Khan,
Karen Madara, Ashani Weeraratana and Dennis D. Taub. CXCR4 Ligation
Increases the Partitioning of Flotillin-1 with Lipid Raft Microdomain and
Plays a Role in CXCR4 Function. European Journal of Immunology, (2007)
8(37), 2104-2116 (Cited as a Cover picture of this issue)
Key Strength:
Experienced in analytical chemistry, troubleshoot common analytical
instruments, extensive knowledge on characterization, formulation of
enzyme kinetics, drug discovery, protein and DNA purification, molecular
biology, microbiology, intracellular protein trafficking, siRNA delivery
technology, inflammatory diseases including atherosclerosis, and
autoimmunity study.