Manager, Molecular Biology, tissue culture, enzyme assays,microbiology
Resume:
Princeton, NJ
SRIDEVI N VISWANATHA ****************@*****.***
SKILLS:
* Experience in Clinical Research, Tissue Culture, Immunology and Molecular Biology, Virology, Microbiology, Stability Coordination Studies, Enzyme Assays and Cell based Assays
* Ability to handle multiple projects with excellent Organizational and Record keeping Skills
* Experience in SOP writing, power point presentations, MS Excel and MS Access
* Has Security Clearance for Federal Government Contracts
Status: US Citizen
Montclair State University, NJ 09/13 to Present
Graduate Student
* Worked on Molecular Biology Certification Program
* Developed model for in vitro knock out of ICER Promoter in Zebrafish cell line using CRISPR/CAS 9 nuclease - Designing and Cloning Oligo’s for guide RNA, Maxi Prep of ICER expression vector DNA, PCR, Transfection, T7 Endonuclease I mutation detection assay to check for ICER promoter deletion.
* Performed Western Blotting and Immunohistochemistry Analysis of transgenic Green fluorescent protein in Zebrafish.
Rutgers State University, NJ 05/13 to 12/13
Volunteer
* Assisted Biology Department as a volunteer to the University
* Performed ATP Bioluminescence Assay to study ATP release patterns during the circadian rhythm in rats.
Novel Laboratories, NJ 03/12 to 08/12
Volunteer
* Loaded, pulled and disposed sample using stability protocol and monitored stability chambers.
* Submitted samples for testing and entered results into Scientek Stability Software.
Pixel Systems Incorporated, NJ 01/04 to 02/12
Manager
* Lead sales team in pre & post sales activities - Increased revenues to $2 million.
* Lead Technical team to meet the project goals and timelines
* Managed HR and accounting team.
* Responsible for Proposal and SOP writing
* Worked with MS Excel and Databases
Duke Medical Center, NC 01/03 to 12/03
Research Analyst
* Performed Cytotoxic T-Lymphocyte assays to study immune responses to tumor RNA loaded patient-specific dendritic cells.
* Maintained dendritic cell lines.
* Performed for LAL-gel clot assay during vaccine administration
University of North Carolina, NC 01/02 to 12/02
Research Analyst
* Assisted in the linkage studies of mutations responsible for Primary Ciliary Dyskinesia to determine mode of transmission.
* Extracted DNA from whole buccal cells and EBV-Transformed cells
* Performed PCR and Gel-electrophoresis and Sequenced DNA using Automated ABI PRSIM 310 SEQUENCER
Bayer Corporation, NC 03/01 to 12/01
Associate Scientist
* Maintained mammalian cell lines; Prepared medium and samples
* Performed viral titrations and In vitro cytotoxicity assays.
* Documented experiments following GMP and GLP guidelines.
* Designed experimental assays and resolved problems
North Carolina State University, NC 08/98 to 02/01
Research Associate
* Worked in Immunology PhD program of College of Veterinary Medicine.
* Responsible for researching and analyzing data and designing experiments
* Performed radioactive and non-radioactive cytolytic T-Lymphocycte assay to study CD8+ T cell suppressor function in FIV challenged cats
* Performed ELISA to detect FIV antigens
* Performed flow cytometry to study T cell immune response to FIV infection in cats
* Isolated PBMC's, macrophages and CD8+ T cells and maintained cell lines
Education
Molecular Biology Certification Montclair State University, NJ 06/14
G.P.A 4.0
M.S. Biotechnology Jawaharlal Nehru Technological University 05/96
G.P.A. 3.7
M.S. Biochemistry Mysore University 07/94
G.P.A. 3.86
B.S. Microbiology/Zoology/Chemistry Osmania University 04/92
G.P.A. 3.98
Awards and Certificates
Introduction to GLP Regulations - Bayer Corporation
Handling Radioactive Isotopes – NCSU
CVM Stipend for the year 1999 – Awarded by College of Veterinary Medicine, NCSU, Raleigh
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