Hong Guo

216-***-****

acfbop@r.postjobfree.com

OBJECTIVE

To obtain a professional position that will utilize my strong technical skills in the area of cancer

biology and drug discovery.

SUMMARY OF QUALIFICATIONS

• Proficiency in cell and animal based drug discovery, variant animal disease models, cell

signaling screening, histology techniques, immunohistochemistry, microscopy, etc.

• Excellent organizational and communication skills.

• Ability to write clear and concise records of experiments

• Strong team player with the ability to work well individually or as a team in a culturally

diverse environment.

WORK EXPERIENCE

Senior Research Associate/ Research Associate: Department Medicine/MHMC, Case

Western Reserve University, 2003-2014

• Discovery of small molecule agonist of EphA2 receptor tyrosine kinase inhibits tumor cell

migration in vitro and prostate cancer metastasis in vivo.

• Discovery of a novel model for dual modality longitudinal imaging of tumorigenesis in liver.

• Establishment and maintenance of multiple lines of genetically engineered mouse models

(GEMM) to interrogate the roles of Eph/ephrin systems in development and diseases.

• Characterization of EphA2 as a novel tumor suppressor in mouse skin.

• Discovery of EphA2 association with development of age-related cataract in mouse and human.

• Discovery of the dual role of EphA2 in colon cancer: Tumor suppressor initiation at early stages

and pro-invasive genes during malignant progression.

• Elucidation of the opposite roles of EphA1 and EphA2 in liver carcinogenesis.

• Characterization of Glial cell line-derived neurotrophic factor (GDNF) is required for

differentiation of pontine noradrenergic neurons and patterning of central respiratory output.

• Characterization of development of pontine noradrenergic A5 neurons requires brain-derived

neurotrophic factor.

Research assistant/Graduate student: Institute of Cell and Biochemistry, Chinese Academy

of Sciences, Shanghai, China, 1999-2002

• Discovery of -Stepholidine promotes proliferation and neuronal differentiation of rat

embryonic striatal precursor cells in vitro.

• Discovery of Apomorphine induces trophic factors that support fetal rat mesencephalic

dopaminergic neurons.

Research assistant/Graduate student: School of Medicine, Southeast University, Nanjing,

China, 1996-1999

• Microinjection of limonene into caudate nucleus inhibits interdigestive migrating motor

contraction of rats.

SKILLS

• Proficiency with various in vivo skills including surgery, microsurgery, dosing (IV, PO),

blood/tissue collection.

• Proficiency with development and characterization of mouse models of disease.

• Proficiency with sterile cell and tissue culture, and with molecular and cellular biology

techniques.

• Familiarized with histological processing including sectioning, cryosectioning and

imunnohistochemistry.

• Experience with in vivo imaging biomarker development using various molecular and cellular

biology techniques

• Experience with protein expression and purification from mammalian cell system, protein

electrophoresis.

• Additional techniques: Manage multiple lines of mouse for large scale disease model and drug

test in vivo.

EDUCATION

Ph.D, Biochemisty 2002

Institute of Cell and Biochemistry, Chinese Academy of Sciences, Shanghai, China.

Master, Medicine 1999

School of Medicine, Southeast University, Nanjing, China

Bachelor, Clinical Medicine 1996

School of Medicine, Southeast University, Nanjing, China

SELECTED PUBLICATIONS

1. Miao H, Gale NW, Guo H, Qian J, Petty A, Kaspar J, Murphy AJ, Valenzuela DM, Yancopoulos

G, Hambardzumyan D, Lathia JD, Rich JN, Lee J, Wang B. EphA2 promotes infiltrative invasion

of glioma stem cells in vivo through cross-talk with Akt and regulates stem cell properties.

Oncogene. 2014 Feb 3.

2. Lee HJ, Hota PK, Chugha P, Guo H, Miao H, Zhang L, Kim SJ, Stetzik L, Wang BC, Buck M.

NMR structure of a heterodimeric SAM:SAM complex: characterization and smanipulation of

EphA2 binding reveal new cellular functions of SHIP2. Structure. 2012 Jan 11;20(1):41-55.

3. Petty A, Myshkin E, Qin H, Guo H, Miao H, Tochtrop GP, Hsieh JT, Page P, Liu L, Lindner DJ,

Acharya C, MacKerell AD Jr, Ficker E, Song J, Wang B. A small molecule agonist of EphA2

receptor tyrosine kinase inhibits tumor cell migration in vitro and prostate cancer metastasis in

vivo.PLoS One. 2012;7(8)

4. Lu X, Guo H*, Molter J, Miao H, Gerber L, Hu Y, Barnes EL, Vogel H, Lee Z, Luo G, Wang B.

Alpha-fetoprotein-thymidine kinase-luciferase knockin mice: a novel model for dual modality

longitudinal imaging of tumorigenesis in liver. J Hepatol. 2011 Jul;55(1):96-102. (*Co-first

author)

5. Jun G, Guo H*, Klein BE, Klein R, Wang JJ, Mitchell P, Miao H, Lee KE, Joshi T, Buck M,

Chugha P, Bardenstein D, Klein AP, Bailey-Wilson JE, Gong X, Spector TD, Andrew T,

Hammond CJ, Elston RC, Iyengar SK, Wang B. EPHA2 is associated with age-related cortical

cataract in mice and humans. PLoS Genet. 2009 Jul;5(7) (*Co-first author)

6. Miao H, Li DQ, Mukherjee A, Guo H, Petty A, Cutter J, Basilion JP, Sedor J, Wu J, Danielpour

D, Sloan AE, Cohen ML, Wang B. EphA2 mediates ligand-dependent inhibition and ligand-

independent promotion of cell migration and invasion via a reciprocal regulatory loop with Akt.

Cancer Cell. 2009 Jul 7;16(1):9-20.

7. Himanen JP, Goldgur Y, Miao H, Myshkin E, Guo H, Buck M, Nguyen M, Rajashankar KR,

Wang B, Nikolov DB. Ligand recognition by A-class Eph receptors: crystal structures of the

EphA2 ligand-binding domain and the EphA2/ephrin-A1 complex. EMBO Rep. 2009

Jul;10(7):722-8.

8. Guo, H., Miao, H., Gerber, L., Singh, J., Denning, M. F., Gilliam, A. C., and Wang, B.

(2006) Disruption of EphA2 kinase led to increased susceptibility to skin carcinogenesis.

Cancer Research 66:7050-7058.

9. Li A, Guo H, Luo X, Sheng J, Yang S, Yin Y, Zhou J, Zhou J. Apomorphine-induced activation

of dopamine receptors modulates FGF-2 expression in astrocytic cultures and promotes survival

of dopaminergic neurons. FASEB J. 2006 Jun;20(8):1263-5.

10. Huang L, Guo H*, Hellard DT, Katz DM. Glial cell line-derived neurotrophic factor (GDNF) is

required for differentiation of pontine noradrenergic neurons and patterning of central respiratory

output. Neuroscience. 2005;130(1):95-105(*Co-first author)

11. Guo H, Hellard DT, Huang L, Katz DM. Development of pontine noradrenergic A5 neurons

requires brain-derived neurotrophic factor. Eur J Neurosci. 2005 Apr;21(7):2019-23.

12. Guo H, Tang Z, Yu Y, Xu L, Jin G, Zhou J. Apomorphine induces trophic factors that support

fetal rat mesencephalic dopaminergic neurons in cultures. Eur J Neurosci. 2002

Nov;16(10):1861-70.

13. Guo H, Yu Y, Xing L, Jin GZ, Zhou J. -Stepholidine promotes proliferation and neuronal

differentiation of rat embryonic striatal precursor cells in vitro. Neuroreport. 2002 Nov

15;13(16):2085-9.

14. Shen Y, Yu Y, Guo H, Tang Z, Yu FS, Zhou J. Identification and comparative analysis of

differentially expressed proteins in rat striatum following 6-hydroxydopamine lesions of the

nigrostriatal pathway: up-regulation of amyloid precursor-like protein 2 expression. Eur J

Neurosci. 2002 Sep;16(5):896-906.

15. Xu L, Sheng J, Tang Z, Wu X, Yu Y, Guo H, Shen Y, Zhou C, Paraoan L, Zhou J. Cystatin C

prevents degeneration of rat nigral dopaminergic neurons: in vitro and in vivo studies. Neurobiol

Dis. 2005 Feb;18(1):152-165.

16. Tang Z, Yu Y, Guo H, Zhou J. Induction of tyrosine hydroxylase expression in rat fetal striatal

precursor cells following transplantation. Neurosci Lett. 2002 May 10;324(1):13-6.

17. Guo H, Zhu XY, Wei YQ, Yang DZ. Microinjection of limonene into caudate nucleus inhibits

IMC of rats. World J Gastroenterol 2000 ;6(Suppl 3)82

MEETINGS AND PRESENTATIONS

Selected speaker: Pharmacology annual retreat, November 10, 2006.

Presenter: Wake Forest University Cancer Center. The First NIH-Sponsored Conference on “Eph and

Ephrin in Cancer”. EphA2 suppressed DEN-induced carcinogenesis in mouse.

2005-2012: Poster presenters, 10th to 15th Research Day, Rammelkamp Center for Research and

Education, MetroHealth Medical Center, Case Western Reserve University School of Medicine. Winner

of Oral Presentations: 16th Research Day, Rammelkamp Center for Research and Education. MetroHealth

Medical Center, Case Western Reserve University School of Medicine September 18, 2013.

The Second NCI-Sponsored Conference on “Eph and Ephrin in Cancer”. Wake Forest University Cancer

Center, North Carolina.

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