Manager Engineering

KATIE LUNDEEN

***** ***** *****, **********, **, 80020

303-***-**** • acezk9@r.postjobfree.com

Professional Summary

Molecular Biology Techniques • Primer Design • Plasmid Design & Construction • Evaluation and Implementation of

New Instrumentation • Project Design • Training • Leadership • Laboratory Automation • Cell Culture

Aseptic Technique • Recombinant Protein Expression • Bioreactor Use • Cell Culture • Fermentation • Yeast &

Bacteria Transformation • Baculovirus Techniques • Enzyme Assay • Protein Characterization • Bradford Assay • Gel

Electrophoresis • SDS-PAGE • Western Blot • Electroporation • FPLC Chromatography • X-Ray Crystallography • GMP

Manufacturing • Dynamic Light Scattering • Light Microscopy • Electron Microscopy • 3-Dimensional Tomography

PROFESSIONAL EXPERIENCE

OPX Biotechnologies• Boulder, CO • January 2013 – May 2014

Sr. Research Associate

Design and construction of microbial strains and plasmids to optimize bio-based chemical pathways. As well as

strain characterization; including evaluation of growth and production, metabolite profiling, enzyme assays, and

protein expression.

! Implementing genetic changes in bacteria to improve production pathways for desired products.

! Designing and constructing complex plasmids.

! Exploring new techniques to improve quality and quantity of strain construction.

! Controlled and maintained strain collection database and stocks.

! Assisted with development and implementation of an electronic notebook system.

Gevo • Englewood, CO • July 2011 – January 2013

Molecular Biologist

Genetically engineering yeast strains to optimize the production of isobutanol as well as high-throughput

cloning of plasmids to facilitate faster strain engineering.

Design, construction and transformation of gene cassettes into yeast strains for improved growth

!

and isobutanol production. Used shake flask fermentation to evaluate strains for isobutanol

production.

Identified and implemented several new instruments to increase throughput of genetic engineering

!

processes and improve data quality.

Worked closely with direct supervisor to create and train a High-throughput Cloning team to

!

streamline genetic engineering process. Directly involved in evolving the group to be more

efficient and productive.

Array BioPharma • Boulder, CO • December 2005 - June 2011

Research Associate

Expression and purification of high-quality recombinant protein for use in assay and co-crystallography

experiments with small molecule drug candidates.

Optimization of quality and yield of protein expression using various expression techniques

!

including baculovirus and insect cells as well as cell bioreactors.

Using affinity chromatography, ion exchange, size exclusion and other protein purification methods

!

to produce large quantities of highly pure protein.

KATIE LUNDEEN • Page 2 • acezk9@r.postjobfree.com

Protein co-crystallography with small molecule drug candidates for evaluation of drug-molecule

!

interactions.

Assisted with development and implementation of several database management tools.

!

Array BioPharma • Boulder, CO • August 2004 – November 2005

Laboratory Technician

Supporting small molecule custom and lead generation libraries through compound analysis, purification and

shipment, as well as participation in GMP manufacturing.

Organizing the delivery of thousands of library compounds per contractual specifications using a

!

variety of laboratory automation and database management.

GMP Manufacturing for Phase I and Phase IIA clinical trials.

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EDUCATION

Molecular, Cellular, Developmental Biology, Minor: Biochemistry • University of Colorado, Boulder, CO

TECHNICAL SKILLS

Microsoft Word, Excel, Access, FrontPage, Outlook, PowerPoint, Internet Explorer, Firefox, Adobe,

Sequencher, Clone Manager, VectorNTI, Biobooks Electronic Lab Notebook, ProMass, Pymol, ChemDraw,

Chemsoft ELN, IMOD Tomography

AWARDS & HONORS

Aknowledgement for work in Three-Dimensional Architecture of Grana and Stroma Thylakoids of Higher

Plants as Determined by Electron Tomography; Plant Physiol. 2011 Apr;155(4):1601-11. Epub 2011 Jan 11

University of Colorado Health Sciences Center Cancer Research Fellowship

Undergraduate Research Assistanceship Program through the Howard Hughes Biological Sciences Initiative

Program

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