Assistant School
Resume:
KEVIN
Z HU
Place,
West
Sacramento,
CA
95691
(530)
304-
5008
********@***.********.***
EDUCATION :
Bachelor
of
Science
in
Microbiology:
Host-
Pathogen
Interactions
University
of
California,
Berkeley
-
2014
SKILLS :
Aseptic
technique
for
bench
and
BSL-
2
biohood
Bacterial
and
Mammalian
(Caco-
2,
FMEC,
MDCK)
Cell
culture
Viral
infection
of
Mammalian
cells
in
plates
and
transwells
Light
and
confocal
microscopy
Immunofluorescence
assays
In
situ
hybridization
using
RNAscope
and
oligonucleotide
methods
DNA
extraction
and
purification
Polymerase
chain
reaction
Gel
electrophoresis
Western
blots
Transformations
using
electroporation
Protein
quantification
assays
Amazon
EC2
Cloud
computing
for
RNAseq
Microsoft
Word,
Excel,
Power
Point,
Outlook
on
Mac
and
PC
Adobe
Powerpoint
and
Illustrator
RELEVANT
E XPERIENCE :
Dr.
Patricia
Pesavento
Lab,
UC
Davis
Veterinary
School
–
Summers
of
2012-
2013
Department
of
Pathology,
Microbiology,
and
Immunology
Student
Research
Assistant
III
• Characterization
of
two
novel
viruses
through
cell
culture
infections
and
a
wide
range
of
biochemical
and
molecular
techniques
• Setup
and
optimized
RNAscope,
a
novel
in
situ
hybridization
procedure,
allowing
single
mRNA
transcript
detection
and
localization
• Designed
probe
and
primer
sequences
for
RNAscope
and
PCR
• Designed
and
executed
an
experiment
to
test
transformed
cells
for
proper
gene
expression
• Maintained
stock
cell
culture
lines
and
necessary
supplies
and
solutions
• Assisted
the
training
of
new
summer
research
students
in
performing
molecular
and
diagnostic
assays
K EVIN
ZHU
PAGE
2
Dr.
Patricia
Pesavento
Lab,
August
2011
Department
of
Pathology,
Microbiology,
and
Immunology
Student
Volunteer
• Studied
the
infectious
preference
of
Feline
Calicivirus
and
Coronavirus
for
apical
and
basal
cell
surfaces
• Planned
and
executed
an
experiment
looking
at
cross-
species
transmission
potential
of
Feline
Calicivirus
in
human
and
dog
cell
lines
through
qRT-
PCR
analysis
• Managed
multiple
cell
lines
simultaneously:
freezing,
thawing,
seeding,
passaging,
counting
• Created
and
compiled
SOPs
and
lab
notebook
entries
• Responsible
for
preparation
and
organization
of
cell
culture
media
and
chemical
solutions
• Autoclaved
glassware
and
ordered
lab
supplies
PUBLICATIONS
A ND
P OSTERS:
L.
Li,
S.
McGraw,
K.
Zhu,
C.
Leutenegger,
S.
Marks,
S.
Kubiski,
P
Gaffney,
F.
Dela
Cruz
Jr,
C
Wang,
E.
Delwart,
and
P.
Pesavento.
Circovirus
in
Tissues
of
Dogs
with
Vasculitis
and
Hemorrhage.
Emerging
Infectious
Diseases.
Volume
19,
Number
4-
April
2013.
Tissue
tropism,
seroprevalence,
and
gene
transcription
of
natural
raccoon
polyomavirus
(RacPyV)
infection
M.
Church,
F.
Dela
Cruz,
K.
Zhu,
K.
Woolard,
P.
Pesavento
Department
of
Pathology,
Microbiology,
and
Immunology.
School
of
Veterinary
Medicine,
University
of
California,
Davis,
CA.
Displayed
at
UC
Davis
2013
Feline
calicivirus
induces
apoptosis
and
is
released
in
membrane
bound
“virosomes”.
D.
Janelle,
F.
Dela
Cruz,
K.
Zhu,
H.
Liu,
and
P.
Pesavento
Department
of
Pathology,
Microbiology,
and
Immunology.
School
of
Veterinary
Medicine,
University
of
California,
Davis,
CA.
Displayed
at
UC
Davis
STAR
conference
2011
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