Manager Supervisor
Resume:
Matthew James
Ryle, Ph.D.
Tel: 207-***-**** Niwot, CO ******@*****.***
Summary
. Driven and goal oriented individual
. Mentor of scientists in both industrial and academic settings
. Friendly, handy and community oriented individual
Experiences
Care Provider for Jerry Ryle Niwot,
CO
2013-April 2014
. Enjoyed the last year of my dad's life until his death in April of
2014
. Read books, assisted with walks, learned about my fathers youth
IDEXX Laboratories Inc. - Product and process development
Westbrook, ME
2003-2013 Scientist 1, 2
Supervisors - Various
. Managed and guided teams of scientists
. Designed and scaled protein purification, conjugation and
immobilization methods
. Identified and isolated biomarkers and antigens from native and
recombinant sources
. Served as chair and member of Technical and Rare Reagent Review Boards
University of Southern Maine
Portland, ME
2011-2013 Lecturer Supervisor -
Stephen Pelsue
. Instructed Chemistry for Health Sciences for 3 years
Used Smart Boards, Power Point and You-tube videos to communicate to
the class
Education
Michigan State University
Lansing, MI
1998-2013 Postdoctoral fellow Supervsor - Bob
Hausinger
Mentored undergraduates, maintained lab equipment and helped manage
lab activities
. Expressed, purified, engineered and characterized oxygen reactive
proteins
Utah State University
Logan, UT
1994-1998 Ph.D. in Biochemistry Supervisor - Lance
Seefeldt
Characterized engineered proteins using spectroscopic methods and
crystallography to understand the nitrogenase catalysis.
. Taught Organic and general chemistry labs and tutored
Fort Lewis College Durango, CO
1989-1994 BS in Chemistry
Taught general chemistry laboratory classes
Conducted undergraduate research, Inorganic Synthesis
Skills
Research and Development
. Hands on experience running and maintaining scientific instruments
. Understanding of animal health and infectious disease.
. Development and automation of immunoassays
. Large scale protein purification and conjugation of proteins and
antibodies
Personal Skills
. Public speaking, teaching and writing
. Fair but driven manager of individuals and classrooms
Mentoring
. Extensive experience training and educating students
. Active role in an intern program aimed at developing and retaining
local scientists
Honors and Awards
7 time recipient of the 2nd highest IDEXX achievement award (The 2X
award) for dedication to intern education, writing outlines of
production, and product innovation
US Patent 7544330 - Microplate sample tracking system - Issued June 9,
2009
NIH post-doctoral fellow (GM20196) entitled "Mechanistic Characterization
of
Taurine dioxygenase". 2000-2002
. Chair and grant writer for the Graduate Research Seminars-Bioinorganic
Chemistry Gordon Conference, Ventura, CA 1999
. Marjorie H Gardner Teaching award, Utah State University, 1998.
Interests and hobbies
Gardening, repairing and riding bicycles, commercial lobster fishing,
milling timber, building tiny homes, foraging, trading with (or helping)
neighbors, backpacking, hiking, and tutoring
Research Articles
22. Grzyska, PK; Ryle, MJ; Monterosso, GR; Liu, J; Ballou, DP; Hausinger,
R.P. Steady-state and transient kinetic analyses of taurine/?-
ketoglutarate dioxygenase: Effects of Oxygen Concentration, Alternative
Sulfonates, and active site variants on the Fe(IV)-oxo intermediate.
Biochemistry. 2005 Mar 15;44(10):3845-55
21. Proshlyakox, DA; Henshaw, TF; Monterosso, GR; Ryle, MJ, Hausinger, RP
Direct detection of oxygen intermediates in the non-heme Fe enzyme
taurine/?-ketoglutarate dioxygenase. J. Am. Chem. Soc. 2004 Feb
4;126(4):1022-3.
20. Ryle MJ, Koehntop KD, Liu A, Que L Jr, Hausinger RP. Interconversion of
two oxidized forms of taurine/?-ketoglutarate dioxygenase, a non-heme iron
hydroxylase: evidence for bicarbonate binding. Proc. Natl. Acad. Sci. U S
A. 2003 Apr 1;100(7):3790-5.
19. Ryle MJ, Liu A, Muthukumaran RB, Ho RY, Koehntop KD, McCracken J, Que L
Jr, Hausinger RP. O2- and ?-ketoglutarate-dependent tyrosyl radical
formation in TauD, an ?-keto acid-dependent non-heme iron dioxygenase.
Biochemistry. 2003 Feb 25;42(7):1854-62.
18. Ryle MJ, Hausinger RP. Non-heme iron oxygenases. Curr. Opin. Chem.
Biol. 2002 Apr;6(2):193-201.
17. Elkins JM, Ryle MJ, Clifton IJ, Dunning Hotopp JC, Lloyd JS, Burzlaff
NI, Baldwin JE, Hausinger RP, Roach PL. X-ray crystal structure of
Escherichia coli taurine/?-ketoglutarate dioxygenase complexed to ferrous
iron and substrates. Biochemistry. 2002 Apr 23;41(16):5185-92.
16. Liu A, Ho RY, Que L Jr, Ryle MJ, Phinney BS, Hausinger RP. Alternative
reactivity of an ?-ketoglutarate-dependent iron(II) oxygenase: enzyme self-
hydroxylation.
J. Am. Chem. Soc. 2001 May 30;123(21):5126-7.
15. Ho RY, Mehn MP, Hegg EL, Liu A, Ryle MJ, Hausinger RP, Que L Jr.
Resonance Raman studies of the iron(II keto acid chromophore in model
and enzyme complexes. J. Am. Chem. Soc. 2001 May 30;123(21):5022-9.
14. Chiu H, Peters JW, Lanzilotta WN, Ryle MJ, Seefeldt LC, Howard JB, Rees
DC. MgATP-Bound and nucleotide-free structures of a nitrogenase protein
complex between the Leu 127 Delta-Fe-protein and the MoFe-protein.
Biochemistry. 2001 Jan 23;40(3):641-50.
13. Grunwald SK, Ryle MJ, Lanzilotta WN, Ludden PW. ADP-Ribosylation of
variants of Azotobacter vinelandii dinitrogenase reductase by
Rhodospirillum rubrum dinitrogenase reductase ADP-ribosyltransferase. J.
Bacteriol. 2000 May;182(9):2597-603.
12. Ryle MJ, Seefeldt LC. Hydrolysis of nucleoside triphosphates other than
ATP by nitrogenase.
J. Biol. Chem. 2000 Mar 3;275(9):6214-9.
11. Ryle MJ, Lee HI, Seefeldt LC, Hoffman BM. Nitrogenase reduction of
carbon disulfide: freeze-quench EPR and ENDOR evidence for three sequential
intermediates with cluster-bound carbon moieties. Biochemistry. 2000 Feb
8;39(5):1114-9.
10. Ryle MJ, Padmakumar R, Hausinger RP. Stopped-flow kinetic analysis of
Escherichia coli taurine/alpha-ketoglutarate dioxygenase: interactions with
alpha-ketoglutarate, taurine, and oxygen. Biochemistry. 1999 Nov
16;38(46):15278-86.
9. Rangaraj P, Ryle MJ, Lanzilotta WN, Goodwin PJ, Dean DR, Shah VK, Ludden
PW.
Inhibition of iron-molybdenum cofactor biosynthesis by L127Delta NifH and
evidence for a complex formation between L127Delta NifH and NifNE. J. Biol.
Chem. 1999 Oct 8;274(41):29413-9.
8. Rangaraj P, Ryle MJ, Lanzilotta WN, Ludden PW, Shah VK. In vitro
biosynthesis of iron-molybdenum cofactor and maturation of the nif-encoded
apodinitrogenase. Effect of substitution for NifH with site-specifically
altered forms of NifH. J. Biol. Chem. 1999 Jul 9;274(28):19778-84.
7. Chan JM, Ryle MJ, Seefeldt LC. Evidence that MgATP accelerates primary
electron transfer in a Clostridium pasteurianum Fe protein-Azotobacter
vinelandii MoFe protein nitrogenase tight complex. J. Biol. Chem. 1999 Jun
18;274(25):17593-8.
6. Ryle MJ, Seefeldt LC. The [4Fe-4S] cluster domain of the nitrogenase
iron protein facilitates conformational changes required for the
cooperative binding of two nucleotides.
Biochemistry. 1996 Dec 10;35(49):15654-62.
5. Ryle MJ, Lanzilotta WN, Seefeldt LC. Elucidating the mechanism of
nucleotide-dependent changes in the redox potential of the [4Fe-4S] cluster
in nitrogenase iron protein: the role of phenylalanine 135. Biochemistry.
1996 Jul 23;35(29):9424-34.
4. Ryle MJ, Seefeldt LC.Elucidation of a MgATP signal transduction pathway
in the nitrogenase iron protein: formation of a conformation resembling the
MgATP-bound state by protein engineering. Biochemistry. 1996 Apr
16;35(15):4766-75.
3. Ryle MJ, Lanzilotta WN, Seefeldt LC, Scarrow RC, Jensen GM.Circular
dichroism and x-ray spectroscopies of Azotobacter vinelandii nitrogenase
iron protein. MgATP and MgADP induced protein conformational changes
affecting the [4Fe-4S] cluster and characterization of a [2Fe-2S] form. J.
Biol. Chem. 1996 Jan 19;271(3):1551-7.
2. Lanzilotta WN, Ryle MJ, Seefeldt LC. Nucleotide hydrolysis and protein
conformational changes in Azotobacter vinelandii nitrogenase iron protein:
defining the function of aspartate 129.
Biochemistry. 1995 Aug 29;34(34):10713-23.
1. Ryle, MJ, Lanzilotta WN, Mortenson LE, Watt GD, Seefeldt LC. Evidence
for a central role of lysine 15 of Azotobacter vinelandii nitrogenase iron
protein in nucleotide binding and protein conformational changes. J. Biol.
Chem. 1995 Jun 2;270(22):13112-7.
References
Phil Andersen, Ph.D. - supervisor Kris Cutis, Ph.D. - Project
manager
Sr. Scientist, IDEXX Laboratories, Inc. Scientist II, IDEXX
Laboratories, Inc.
******-********@*****.*** ********@*****.***
Joel Goodness - team member Stephen Pelsue, Ph.D. - Supervisor
Scientist I, IDEXX Laboratories, Inc Associate Dean, University of
Southern Maine
***********@**********.*** ******@***.*****.***
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