Assistant Maintenance

ANURA SHRIVASTAVA

**** ********* ****, ********* ** ? 201-***-**** ?

*****.***********@*****.***

Education

Master of Science, Biotechnology, Aug 2011

Wayne State University, Detroit, MI

Bachelor of Technology, Biotechnology, May 2009

Dr. D.Y.Patil Biotechnology and Bioinformatics Institute, Pune,

India

Skills

. Genotoxicity studies: invitro mammalian chromosomal aberration test,

mouse lymphoma assay plate counts, bacterial reverse mutation plate

counts, cytotoxicity studies

. Mammalian cell culture: cell culture passaging, cryopreservation,

transfection, developing stable lines

. Virology techniques: construction and purification of recombinant

viral vectors, plaque assay, virus infection and titration,

recombination of KO5 constructs in BAC system

. Molecular Biology techniques: PCR, cloning, southern blot, plasmid

extraction, in vitro transcription, gel electrophoresis, BAC

extraction, SDS-PAGE, western blot, spectrophotometer experience

. Microscopy experience: Scoring chromosomes spreads, injecting ds RNA

in larvae, collecting insect brain tissue for deep sequencing

. Microbiology techniques: microbial culture, biochemical tests,

microbial staining, bacterial inoculations

. Plant Tissue Culture technique: micropropagation and in vitro

proliferation of explants, callus culture

. Laboratory skills: maintenance of lab inventory and equipment, BSL-1 &

BSL-2 laboratory safety, follow SOPs, record data and interpret

results, GLP/GMP compliance, ISO regulation

. Computer skills: LIMS, Track-wise, MS Office, MS Project, Lotus Notes

. Bioinformatics tools and databases: BLAST, Clustal-W, RasMol, NCBI,

PDB, GenBank, RefSeq

Professional Experience

Principal Technologist - North American Science Associates,

Northwood, OH Jan 2013-Present

. Primarily involved in performing invitro compatibility testing such as

mammalian chromosomal aberration test using CHO-WBL cell line

. Also performs absorption capacity test and cytotoxicity studies

(agarose overlay method, ISO elution method, direct contact method)

using L-929 fibroblast cell line

. Proficient in mouse lymphoma assay plate counts using L5178 TK cell

line and bacterial reverse mutation plate counts

. All methods are performed in GLP/GMP environment and in compliance

with ISO 10993

. Independently conducts assigned test procedures including laboratory

scheduling, testing, generating and analyzing data, interpreting

results

. Assists in the development and improvement of SOPs and developing

study designs and protocols

. Responsible for following protocols, SOPs and relevant compliance

regulations in regards to assay and safety procedures

. Accurately recording raw data in logbooks, worksheets as well as

particular studies in LIMS (laboratory information management system)

. Routing all positive results or unusual testing occurrences to

management and initiate investigations/unexpected result observation

using Track-wise program

. Involved in overall maintenance of laboratory including scientific

equipment, instrumentation, media and reagent preparation

. Assists in training new associates in performance of routine lab tasks

and involved in client contact and participation in client audits

Research Assistant- Wayne State University, Detroit, MI

Oct 2011 - Dec 2012

. Primary duties involved passaging laboratory cell lines such as U2OS,

Vero, Hep-2, 293

. Performing mammalian cell culture techniques like propagation and

maintenance of cell lines, developing stable lines, freezing and

thawing cells

. Involved in Herpes Simplex Virus-1 (HSV-1) research

. Performing virology techniques such as transfection & infection, BAC

DNA titration, plaque assay and plaque counts

. Carrying out all experiments in an aseptic environment

. Responsible for overall laboratory management including ordering

laboratory supplies, maintaining inventory, laboratory safety and

waste/hazardous records

. Training undergraduate and graduate students

Teaching Assistant (Microbiology) - Wayne State University, Detroit,

MI Aug 2009 - Aug 2011

. Lectured Microbiology lab course (BIO2200)

. Training students in various microbiology techniques such as microbial

staining (gram, endospore, acid fast, capsular and negative staining),

aseptic techniques, bacteriophage assay

. Demonstrating various tests such as MIC test, oxidase test, coagulase

test, catalase test, antibiotic susceptibility testing, motility test,

media preparations, bacterial inoculations, unknown bacteria

identification, differential and selective media, E test,

electroporation, gene cloning and replica plating

Research Experience

Identification of dimerization domain of ICP0 and motifs involved in

ICP0 translocation

Wayne State University, Detroit, MI

Oct 2011 - Dec 2012

. Performed cloning of different fragments of ICP0 into KO5 vector,

followed by cloning of KO5 into ICP0 null BAC

. Transfected BAC DNA in U2OS cells and screened for recombinant single

plaques by multiple rounds of infection and extraction

. Confirmed the recombinant viruses by PCR/southern blot, followed by

amplification of the recombinant virus

Deep transcriptome analysis of Tribolium castaneum eye development

Wayne State University, Detroit, MI (MS Thesis)

May 2010 - Aug 2011

. The aim was to investigate the eye development pathway in Tribolium

castaneum, red flour beetle

. Generated and collected knockdown tissues of the early retinal

genes using siRNA

. Samples were sent for deep RNA sequencing, followed by

transcriptome analysis

. Studied differential expression profiles of validation genes in

knockdown transcriptomes

Studies on greenhouse production of Crocus sativus L in non-natural

habitat

National Chemical Laboratory, Pune, India

Jun 2010 - Aug 2010

. The objective of the study was to standardize parameters for

greenhouse cultivation of saffron

. Replacement corms were produced by in vitro induction of micro-corms

by tissue culture methods

. Various parameters such as size and weight of replacement corms,

presence of stigma metabolites, effect of sucrose, temperature and

light on replacement corms were studied

Presentations

. Poster presentation: "Functional genomic analysis of eye development

in red flour beetle Tribolium castaneum", Summer Symposium on

Transcriptional Dynamics, Evolution and Systems Biology, Michigan

State University, Lansing, July 2011

Contact this candidate