College Project

Curriculum Vitae

Kuhu Roy

Ms. Kuhu Roy

**** ****** *****

San Jose, CA 95148

Ph: 408-***-**** (Home)

408-***-**** (Cell)

Email: ********@*****.***

OBJECTIVE

Looking for an entry level position in a Biotechnology Industry, specially related to direct hands on

works in Labs.

EDUCATION

Bachelor of Science (BS, two years college degree), Calcutta University, India

Major: Biological science, Chemistry

Associate in Biotechnology: Calhoun Community College, Huntsville, AL (Collaborated with Hudson

Alpha Institute of Biotechnology) with overall GPA of 3.954, Major GPA is 4.0. The course has been

specially designed by Hudson Alpha Institute of Biotechnology indented to develop fundamental

knowledge and strong lab skill in Biotechnology field for the students.

All the major courses are complete d. Expected graduation: by summer 2014.

Major courses taken :

Biology (BIO 103), Introduction to Biotechnology (BIO 105), Cell Culture (BIO-107), Techniques in

Molecular Biology (BIO-203), Directed Studies in Biotechnology (BIO-252), Chemistry (CHM 111

and CHM 112), Pre-calculus Mathematics (MTH 112), Advanced topics in Biotechnology (BIO-254,

a college project ) a nd Biotechnology Internship (BIO-256, in Thermo Fisher Scientific), Genetics

219.

INDUSTRIAL EXPERIENCE

Summer Internship ( through Biotrain scholarship, Hudson Alpha I nstitute of Biotechnology) a t

Thermo Fisher Sceintific in R & D lab during summer 2010. My work primarily involved DNA

purification and analysis, Protein purification, expression, analysis, and Transfection.

Full Internship in Thermo Fisher Scientific Production lab from October 2010 till October 2011.

SKILLS

I gained good laboratory experience in the biotechnology area and developed different technical skills.

I am confident to work independently as well as in a team.

I have experience a nd skill in the following techniques.

Solution Preparation, Pipeting (extensive use of Pipets and Micro -pipets), use of

balances, spectrophotometers, etc.

Cell Culture ( maintaining different aseptic techniques)

1. Bacterial and mammalian cell growth and maintenance

2. Use of cell culture in assays

3. Preparing bacterial media

4. Serial dilutions

Molecular Biology:

1. Genomic and Plasmid DNA purification

2. Bacterial transformation with Plasmid DNA

3. PCR amplification of genes of interest

4. PCR primer design

5. Ligation of genes of interest into Plasmid DNA to facilitate analysis of the gen e

6. Selection of Plasmids containing successfully subcloned genes of interest

7. Restriction enzyme analysis of Plasmid DNA

8. Preparation of DNA sequencing reaction

9. DNA transfection

10. Preparation of RNA from mammalian cells and reverse transcriptase -PCR analysis

11. Assessment of protein expression in transfected mammalian cell

12. SDS-polyacrylamide gel analysis of protein

13. Western blot and ELISA immunoassay

SPECIAL AWARDS

Received PBR (Partner for Biotechnology Research) Scholarships two times, one in 2010

and the other in 2011 from Hudson Alpha Institute of Biotechnology.

Summer Biotrain Internship from Hudson Alpha Institute of Biotechnology in 2010 and

2011

Selected to be included in Who’s Who Among Students in American Junior Colleges from

Calhoun Community College.

PRESENTATIONS

Poster: Analysis of Fusion Protein Localization, Hudson Alpha Institute of Biotechnology, July 30,

2010 (in Biotrain 2010).

PROFESSIONAL ORGANIZATION:

Member of Phi Theta Kappa.

CERTIFICATE

Certificate of Excellence in Biotech 101 from Hudson Alpha Institute of Biotechnology (September

2010)

WORK STATUS

Permanent resident (Green Card holder) of Unites States.

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