Project Training
Resume:
ARTUR ROMANCHUK Ph.D.
Westfield, New Jersey 07090
*****.*********@*****.***
EDUCATION
University of North Carolina Chapel Hill Ph.D., Cell Molecular Biology April 2014
The College of New Jersey BS, Biology May 2008
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SUMMARY
SAMtools
R (nlme, lme, lattice)
DNA/RNA sequence library prep
DNA/RNA purification
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BWA
UNIX BASH
PCR/qPCR/rtPCR
Sterile technique Cell line design
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ClustalX
Perl
Flow cytometry/FACS
Protei cloning/expression
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BLAST
PowerPoint
Fluorescent microscopy
STRING/KEGG/GenBank
! Cell based assay
BLAT
MicrosoftWord
ELISA/WESTERN
CELL and MOLECULAR BIOLOGY EXPERIENCE
(2011-2014) Senior project lead developed application of cell biology, genetics and molecular
biology to cell culture and expression of (4123 E.coli, V.cholera, MRSA) proteins in screening
and subsequent process development.
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(2008-2014) Technical lead developed cell culture media and optimization using empirical and
statistical methods.
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(6+ years)Served as technical expert in understanding of cell biology, biochemistry, and
metabolic (specifically sugar metabolism and fatty acid metabolism) analysis.
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(2011-2014) Project lead developed protein bioengineering methods resulting in scale-up
experimental
design.
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(6+ years)Technical expert in sterile laboratory cell culture processing techniques and protein
chemistry.
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(2010-2014) Project lead designerd and organized and executed multiple complex
molecular scree and protein engineering experiments simultaneously. Along with cell
population characterization experiments using next generation sequencing (Illumina
multiplex).
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(2009-2014) Bioinformatic lead Planned and maintained bioinformatic and molecular project
workflow and organize workflow for junior colleagues.
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While at UNC -Chapel Hill worked across group and department boundaries, collaborated
with UNC sequencing core facility and University of Arizona to
chieve project objectives.
a
(4 years) Lead research scientist compiled, organized, evaluated and published data and
conceived of novel ideas from the data using statistical tools.
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(2008-2014) As a project lead prepared experimental and statistical reports and presentations
for internal and external groups. Demonstrated effective written and oral skills through
publication
of a number of per reviewed articles and presentation of talks at national and
international conferences.
TECHNICAL EXPERIENCE
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Ph.D. in Cell and Molecular biology (curriculum in genomics and
bioinformatics).
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(6 years)Strong understanding and proficiency with alignment software
(SAMtools, BWA, ClustalX, BLAST, BLAT).
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(3 years)Statistical R Programing (regression modeling, mixed linear
models, principal component analysis, graphics).
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(6 years) Strong high throughput sequencing genomic big data (Next
Generations sequencing) analysis experience (Microbial population structure
identification, DNA identification, RNA expression quantification).
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(3 years)Extremely competent in quantitative (regression analysis) and
qualitative (trend setting and survival) analysis, regression, data manipulation
in R, critical thinking and evaluation of experimental design.
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(4 years) Experience distilling large amounts of experimental information into
key findings leveraging available genomic databases (KEGG, STRING,
GenBank).
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(5 years)Excellent communication skills, experience in explaining complex
systems both to internal teams and at national and international
conferences.
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(5 years) Strong Data Visualization experience on prior microbial genomics
"Big Data" projects (R graphics lattice package, Adobe Photoshop and Adobe
Illustrator).
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(5 years) Statistical background and training in classical statistics as they apply
to population interactions with the environment.
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LEADERSHIP SKILLS
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Led department wide “Slide design and the telling of the scientific
story.”
workshop (2013 annual UNC Biology Symposium)
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Lead weekly performance updates and Department Seminars
(2012-2013)
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Independent funding acquisition from NSF and private
foundations
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Contributed to Carolina Scientific scientific magazine (expert interview
2014)
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Group leader - undergraduate team of 8 team members for over three
years, resulting in a publication and three undergraduate honor thesis
projects
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SOFTWARE SKILLS
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Genomics Data analysis Perl, R, (BWA, SAMtools, SAS, familiarity
with Python, Ruby, MySQL architectures )
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Mathematical network modeling.
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UNIX Terminal, Excel, Word
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Experience with MatLab,Photoshop, Illustrator.
INSTRUMENTATION and PROTOCOL TRAINING
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Gel based molecular assays
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Fluorophore based molecular assays
Cell Flow Cytometry - BD Accuri C6 Flow Cytometer
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DNA/RNA - Caliper Life Sciences LabChipGX
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RT/Q PCR - BioRad CFX90 Real-Time Systems
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PCR - epGradient MasterCycle Eppendorf
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Proprietary Chemostat Set up
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Microscopy (fluorescent/confocal/scanning electron) - Leica MZ75
COMMUNICATION SKILLS
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Oral and written project description and data summary (published
research)
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Oral presentations to US and international conference audiences
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Led workshops for scientific peer audiences
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Undergraduate Lecturer (8 semesters)
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Fluent in oral and written Russian and Ukrainian
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PUBLISHED WORK
1Paper High number of protein interactions is not a barrier to HGT in Escherichia coli.
Romanchuk A, Burch CL, Jones CD, in prep. Submitted in May 2014.
2Paper Bigger is not always better: fitness burden and transmission of ~1MB Pseudomonas syringae
megaplasmid pMPPla107.
Romanchuk A, Jones CD, Baltrus DA, PLASMID May 2014.
Paper RipA modulates the acyl chain specificity and stability of LpxA in Francisella tularensis.
Cheryl N. Miller1, Shaun P.
teele1, Jason C. Brunton1, Ronald J. Jenkins2, Eric D.
S
LoVullo 1, Sharon A. Taft-Benz1,
Artur Romanchuk3, Corbin D. Jones3,4, Garry D.
Dotson2, Edward J. Collins1 and Thomas H. Kawula.1.Journal of Biological
Chemistry in review October 2013.
Paper Suppressor Analysis Reveals a Role for SecY in the SecA2-Dependent Protein Export Pathway of
Mycobacteria.
Ligon LS, Rigel NW, Romanchuk A, Jones CD, Braunstein M. J Bacteriol. 2013
Oct; 195(19):4456-65.
Paper Dynamic evolution of pathogenicity revealed by sequencing and comparative genomics of 19
Pseudomonas syringae isolates.
Baltrus DA, Nishimura MT, Romanchuk A, Chang JH, Mukhtar MS,
Cherkis K, Roach J, Grant SR, Jones CD, Dangl JL. PLoS Pathog. 2011 Jul, 7(7).
Poster Fitness cost of ~4000 horizontally transferred Escherichia coli genes.
Romanchuk A, Jones CD. 2013
Poster High number of protein interactions is not a barrier to HGT in Escherichia coli.
Romanchuk A Jones CD, Burch CL. 2013
Poster Gene gain via massive horizontal transfer: Pseudomonas syringae 1Mb Mega plasmid.
Romanchuk A, Baltrus DA. 2012
Poster Evolution of pathogenicity revealed by sequencing and comparative genomics of 19
Pseudomonas
syringae isolates.Romanchuk A, Baltrus DA, Jones CD. 2009
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CONFERENCE PRESENTATIONS
2014
Presentation “Experimental Measures of the Costs of Complexity for
Horizontal Gene Transfer” to Evolution 2014 joint annual meeting of the
Society for the Study of Evolution, American Society of Naturalist and the
Society of Systematic Biologists, Raleigh Convention Center, North Carolina.
Featured Article: Carolina Scientific magazine (pg29), “The protein network:
predicting antibiotic resistance with metabolic pathways.” written by Travis
Murphy, Spring 2014 issue, http://issuu.com/uncsci.
2013
Presented poster “Fitness cost of ~4000 horizontally transferred
Escherichia coli genes.” to South Eastern Population Ecology and
Evolutionary
Genetics Conference - workshop.
Presented poster “High number of protein interactions is not a barrier
to HGT in Escherichia coli”. to the Gordon Research Conference (GRC)
Microbial Population Biology and Evolution - workshop.
2012
Presented poster “Gene gain via massive horizontal transfer:
Pseudomonas syringae 1Mb Mega plasmid” to South Eastern Population
Ecology and Evolutionary Genetics Conference.
Presentation “Genetic super mover: Pseudomonas syringae 1Mb Mega
plasmid” Evolution 2012 joint annual meeting of the Society for the
Study of Evolution (SSE), Ottawa, Ontario, Canada.
2010
Presentation “Horizontal Gene Transfer: a Persisting Source of
Adaptation” Evolution 2010, Society for the Study of Evolution (SSE),
and the American Society of Naturalists (ASN), Portland Oregon.
2009
Presented poster “Evolution of pathogenicity revealed by sequencing
and comparative genomics of 19 Pseudomonas syringae isolates” to
South Eastern Population Ecology and Evolutionary Genetics
Conference.
2007
Presented poster “Test of The Enemy Release Hypothesis: Andropogon
virginicus and its fungal parasites.” to American Society of Plant
Biologists (ASPB), American Society of Plant Taxonomists (ASPT),
Botanical Society of America (BSA) Chicago, Illinois.
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EXPERIMENTAL TRAINING
- Genomics NextGen assay analysis and development
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Protein expression and analysis and cell line engineering skills
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Plasmid based and viral-based expression methodologies
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Flow cytometry.
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Aseptic cell culture skills, microbial phenotype optimization
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Statistical data analysis (lm/glm/anova/manova/pca/t-test)
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DNA/RNA Detection, Extraction and Purification
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DNA Preparation for High Throughput Sequencing
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Robotic automation, set up and run bioassays for screening and potency
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THEORETICAL TRAINING
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Microbial population Ecology & Evolution (pathogen host interaction)
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Microbial Environmental Ecology
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Microbial Genetics
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Molecular/ Cell Biology
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Genetics/ Population Genetics
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Computational Genomics and High Throughput Sequence Analysis
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Biostatistics, GLM, Anova, PCA analysis
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