Assistant Engineering

Hongfeng Chen

**** ******** **. ******** ** *****

Phone: 847-***-**** Email: aceoc3@r.postjobfree.com

SUMMARY

A passionate biomedical engineer/scientist with thorough knowledge of molecular and cellular

biology and over 10 years of hands-on experience in a wide variety of bio analysis products and

instruments. A breadth of expertise includes: Microscopy • Image Acquisition and Analysis •

System Integration • Flow Cytometry • PCR • Spectrophotometry • Laser Optical Tweezer •

Western Blotting • Cell and Tissue Culture • Transient and Stable transfection • Molecular

Cloning • Cell Physiology • Pathology • Programming • Mathematical Modeling • Nanopartical

Education:

Ph. D in Bioengineering 2007

University of Illinois at Chicago, Chicago, IL

M.S. in Biomedical Engineering 2002

Zhejiang University, Hangzhou, Zhejiang, China

B.S. in Biomedical Engineering 1999

Zhejiang University, Hangzhou, Zhejiang, China

Experience:

Postdoctoral researcher – Department of Pathology, University of Tennessee Health Science

Center, Memphis Tennessee current

• Established a system based on flow cytometry and confocal microscope to evaluate cell

apoptosis/necrosis

• Developed a protocol and established a system for molecular cloning using basic

molecular method, such as PCR, nucleic acid extraction and agrose gel electrophoresis

• Established a fluorescent liposome or giant unilamellar vesicles (GUV) system to

research protein-lipid interaction based on confocal microscope and electroporation.

• Performed recombinant protein expression and purification in E. coli

Postdoctoral scholar – Department of Surgery, University of Chicago, Chicago IL, 2007-2009

• Established a ultrasound system to induce cell membrane injury

• Developed a system based on flow cytometry and fluorescent microscope to

quantitatively evaluate the cell membrane resealing

• Developed a animal model to test the police electrical tazer effect on nervous system

• Investigated mechanisms of poloxamer 188 to seal cell membrane

Research Assistant – Cell and tissue engineering optical lab, Department of Bioengineering,

University of Illinois at Chicago, Chicago, IL, 2003-2007

• Establish an imaging system to monitor the quantum dots movement in cell membrane

and developed a method to quantitatively analyze the movement change

• Investigated cell membrane reservoir using laser optiacal tweezer

• Built a mathematical model to explain the cell membrane protein movement change

Research Assistant - Biomedical functional image and Neuro-engineering lab, University of

Illinois at Chicago, Chicago, IL, 2002-2003

• Developed software using C and Fortune for image analysis.

• Performed rabbit cardiovascular function experiment.

Research Assistant - Zhejiang heart cerebrovascular, nervous system development and drug

screening laboratory, Zhejiang University Hangzhou, Zhejiang, 1999-2002

• Setup an in vivo (rat and mouse) image system to analyze small animal microcirculation.

• Evaluated at least 3 traditional Chinese medicines using in vivo thrombus experiments.

• Developed software (using C and Delphi) to calculate the velocity of the blood cells from

the images acquired; the same software was also used to track the route of rats in the

water maze.

• Animal care technician (rat, mouse, Guinea pig)

Teaching Experience

Teaching Assistant – Bioengineering Department, University of Illinois at Chicago, Chicago, IL,

2003 Course: Pattern Recognition

Collaborate on curriculum and exam development, tutor undergraduate students, correct

homework including programming problems, graded exams.

Lab training: 3 Ph. D students in UTHSC campus, 2 technicians in University of Chicago

Honors and Awards

Outstanding Student Scholarship, 1995-1999

Excellent Student of Zhejiang University, 1995-1999

Honored Graduate Student, 1999

Travel Award, 2005

Selected Publications

1) Jason Workman, Hongfeng Chen and R. N. Laribee. Environmental signaling through the

mechanistic target of rapamycin complex 1- mTORC1 goes nuclear. Cell Cycle 2014 Feb

7;13(5). [Epub ahead of print]

2) Hongfeng Chen, Jason Workman, Alexa Tenga, and R. Nick Laribee. Target of rapamycin

signaling regulates high mobility group protein association to chromatin, which functions to

suppress necrotic cell death. Epigenetics Chromatin. 2013 Sep 2;6(1):29

3) Sudeep George, Hongfeng Chen, Jacinta Conrad and Seema Khurana. Regulation of

directional cell migration by membrane-induced actin bundling. J Cell Sci. 2013 Jan 1;126(Pt

1):312-26.

4) Hongfeng Chen, Meiyun Fan, Larry Pfefferm and R. Nick Laribee The histone H3 lysin 56

acetylation pathway is regulated by target of rapamycin (TOR) signaling and function directly in

ribosomal RNA biogenesis. Necleic Acid Research, 2012 40(14):6534-46

5) Julianna Oliveira, Hongfeng Chen, Michael Cho and Raphael Lee The Copolymer surfactant

P188 Reduced Tension in Permeabilized Cell Membrane. Biophysical Journal 100(3) Sup

February 2011 326a-327a

6) Hongfeng Chen, Igor Titushkin, Michael Stroscio, Michael Cho. Altered membrane dynamics

of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow

derived progenitor cells. Biophysical Journal 2007 92(4):1399-408

7) Peng Shi, Hongfeng Chen, Michael Cho, Michael Strosico Peptide-directed binding of

quantum dots to integrins in human fibroblast, IEEE Trans Nanobioscience. 2006 Mar;

5(1):15-9

8) Dimitri Alexson, Hongfeng Chen, Michael Cho, Mitra Dutta, Yang Li, Peng Shi, Amit

Raichura, Dinakar Ramadurai,Shaunak Parikh, Michael A Stroscio and Milana Vasudev.

Semiconductor nanostructures in biological application. Journal of Physics: Condensed

Matter Volume 17 (2005), Number 26 R637:R656

9) Xiangping Wu, Hongfeng Chen, Weiming Yan and Xianoxiang Zheng, Establishment of a

system for measuring blood flow velocity of rat microvessel using dark background fluorescent

image analysis method, Biomedical Engineering Journal (China) 2005 Oct; 22(5):1063-6

10) Hongfeng Chen, Michael Cho Single particle tracking of quantum dot-conjuaged integrin

dynamics on human mesenchymal stem cell. 2005 Annual Fall Meeting at Biomedical

Engineering Society

Book chapter:Chapter 27: In Vivo Electroporation: An Important Injury Mechanism in Electrical

Shock Trauma. Advanced Electroporation Techniques in Biology and Medicine 2010

Presentation:

• Single particle tracking of nanoscale integrin dynamics using quantum dots. The 23rd

Society for Physical Regulation in Biology and Medicine (SPRBM) Jan. 2005 Lake

Tahoe, California

• Mechanisms of restoring structure and viability to disrupted cells. The 4th Annual

Academic Surgical Congress (ACS) Feb. 2009 Fort Myers, Florida

• Poloxamer 188 Restores the Detergent Depleted Membrane Reservoir Access. The 49th

Midwestern Association of plastic surgeons (MAPS) meeting. April 2009 Chicago, IL

• AFM Evidence of Poloxamer Annealing of Detergent Lysed MDCK Cells. The 49th

Midwestern Association of plastic surgeons (MAPS) meeting. April 2009 Chicago, IL

• Target of rapamycin complex 1signaling regulates chromatin association by HMG

proteins to prevent necrotic cell death. The 20th Annual Southeastern Regional Yeast

Meeting (SERYM) March 2013 Birmingham AL

• Histone 3 lysine 37 plays important role in TORC1 pathway to promote HMG proteins

associate with chromatin. Annual University of Tennessee Health Science Center postdoc

research meeting 2014 Memphis TN

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