CURRICULAM VITAE

MOB;+917*********

aceisk@r.postjobfree.com

aceisk@r.postjobfree.com

SAJITH.V.S, Master of Science in Bio Technology

CAREER OBJECTIVE

To work under challenging assignment and to contribute the best of my effort for the growth of

the organization.

Research Experience

Currently working as a Research and Development assistant In Jawaharlal Nehru Center For

Advanced Scientific Research (JNCASR) Bangalore, under Dr.Amithabh Joshi (Evolutionary

And Organismal Biology Laboratory) previously worked as a Research and Devel opment

assistant In Indian Institute of Horticulture Research (IIHR), Bangalore

Work done,

1 Developmental studies using drosophila

2 Estimation of lipid contents in drosophila

3 Molecular studies (RNA, isolation, RT PCR, PCR)

Project work in MSc,

Development Of Monoclonal Antibodies Against Downy Mildew Through Phage

1

Display Technology And Molecular Evaluation of Transgenic Tomato

Indian Institute of Horticulture Research (IIHR), Bangalore

Institute

Student

Role

May2005 – November 2005

Duration

Dr.Akella Vani, Principal Scientist

Under the

Guidance

The main objective of the project was to produce single chain antibodies to detect Downy

Mildew through Phage Display Technology, screening of transgenic plants, cloning of TLCV

Replicase gene in to puc-18 vector

Work done,

The polyclonal antibodies against downey mildew of grapes were generated using phage

display library by biopanning procedure.

Fifty monoclonal antibodies specifically binding to pure antigen was screened by ELISA.

Later out of these fifty monoclonal antibodies six antibodies were screened having high

affinity to infected leaf samples.

The monoclonal antibody gene having higher affinity to infected tissue was sub cloned

into alkaline phosphatase construct (pHOA)

The specificity of the construct (pAV Mab-Alp) was confirmed by DAS-ELISA.

The Dip-stick protocol was developed using the above construct as secondary antibody to

detect the infection in specific crop.

Technical Expertise

Email:

Molecular Biology : Plasmid Isolation,Cloning and Screening

aceisk@r.postjobfree.com

Expression in E. coli System, Agarose Gel

phone:

Electrophoresis, SDS Page, RT-PCR, DOT –

+91-725*******

BLOT,SOUTHERN BLOT, DIBA,Genomic

Present Address

DNA isolation, ELISA, Chromatography

JNCASR, EOBU

techniques

Jakkur,Bangalore

Plant Biotechnology : Tissue culture

Evolutionary biology: Developmental studies with drosophila

Permanent Address

Education

Venganilkumthodiyil

(H) Master of Science in Biotechnology, St Philomena’s college

Athirattukunnu(PO) Mysore, Punjab Technical University

Kenichira I Sem II Sem III Sem IV Sem

Wayanad(DT) 69.66% 74.80% 71.20% 88%

Kerala, PIN-673596

Bachelor of Science (BioChemistry, Botany & MicroBiology)

Personal Data Sharadha vilas college mysore, Mysore University

D.O.B: 25/05/1981

I year II year III year

Nationality: Indian

Languages: English, 59.33% 55.00% 56.00%

Kannada, Hindi,

Pre Degree Course,Calicut university

Malayalam

(Biology, Physics, Chemistry)

Interests:

Travelling, Cricket, SSLC (Kerala Board)

Listening music

Seminar presentations

Seminar on Plasmid as Cloning Vector

Seminar On DNA Repair Mechanisms

Seminar Presentation On Transformation

Paper presentations

Akella Vani, Jagadish, K, Chandrashekara, K.N, Krishnamurthy, K.B, Rashmi, S, Sajith,V.S

and Vinaya.K. 2005. Generation of single chain antibody fragments (scFv) specific

for nucleocapsid protein of peanut bud necrosis virus (PBNV) infecting tomato using

phage display library. Presented in: National Symposium on 'Transgenic crops in pest

management', 12-13th Sep 2005, Centre for plant molecular biology, Tamil Nadu

Agricultural University, Coimbatore, India. Pp: 31

Binesh K.J, Sajith V.S, Libin Kumar K.V,Anoop Joseph. medicin from toxins,National

Symposium On Venoms And Toxins department of biochemistry held at mysore

university 2004

s

Jagadish, K,Chandrashekara, K. N, Sajith V., Rawal, R.D., and Akella Vani. 2007.

Development of a dipstick diagnostic method for detection of downy mildew in

grapes caused by Plasmopara viticola using single chain antibody fragments.

Advances in Downy

Mildew Research. Proceedings of The 2nd International DownyMildwes Symposium

heldon4-7thJuly2007atPalackyUniversity in Olomouc, Czech\Republic.(3)Pp:79-83

Oral presentation:

s

Jagadish, K,Chandrashekara, K. N, Sajith V., Rawal, R.D., and Akella Vani. 2007.

Development of a dipstick diagnostic method for detection of downy mildew in

grapes caused by Plasmopara viticola using single chain antibody fragments.

Presented in: The 2nd International Downy Mildwes Symposium held on 4-7th July

2007 at Palacky University in Olomouc, Czech Republic Pp: 79-83

REFERENCES

1 Dr Amithab joshi

Evolutionary Biology Laboratory

Evolutionary & Organismal Biology Unit

Jawaharlal Nehru Centre for Advanced Scientific Research

Jakkur P. O., Bangalore 560 064, INDIA

2 Dr. Akella Vani

Principal Scientist

Department of Biotechnology

IIHR, Hessargata Lake Post,

Bangalore-89

E-Mail: aceisk@r.postjobfree.com

3 Fr, Leslie moras

Principal

St, Phelomenas college

Mysore-17

4 Prof. Gururaj

Head of Microbiology Department

Sharadha Vilas college

KM Puram, Mysore

I hereby declare that all the statements made above are true, complete and correct to the

best of my knowledge and belief.

Place :Bangalore

Date :

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