Mamudou S Bah

**** ********* ******* **, *******, North Carolina 27604

Cell Phone #: 919-***-****, Email acei6l@r.postjobfree.com

Professional Objective: Driven, goal-oriented team player and seeking any challenging position in

the field of Biotechnology and Pharmaceutical industries to further enhance and develop my educational

experience

Education

North Carolina Central University (August 2010 - Dec 2012)

Durham, North Carolina

Department of Pharmaceutical Sciences; Biomanufacturing Research Institute and Technology enterprise

(BRITE)

MS degree in Pharmaceutical Sciences

Relevant Graduate level Courses: Drug Discovery and Development, Advanced Protein Chemistry,

Enzymes Kinetics, Assay Design and Development Technology, Bioinformatics, Advanced Biochemistry,

Cloning and Expression, Pharmacology and physiology, plant genomics, and Biopharmaceutical

manufacturing

Rust College (January 2006 - May

2008)

Holly Springs, Mississippi

BS degree in Biology

Relevant Courses: Microbiology/Immunology, Molecular Biology, Biochemistry, Genetics.

Laboratory Skills:

• Agrcbacterium – mediated plant transformation

• Plant tissue culture

• Protein Extraction and Purification

• Western blotting analysis, ELISA

• DNA and RNA isolation

• PCR, RT- PCR, QRT- PCR

• Growing and maintaining plants in greenhouse

Computer Skills:

• Microsoft words, Excel, Power points

• Blast, Fasta, Clustal W

Professional Experience

Bioprocess Technician-Vaccine: Merck (October 2012 to present)

• Perform Manual and automated operations

• Trained and qualified in aseptic manipulation

• Revises and authors SOPs and cGMP documentation.

• Provides all documentation and clerical functions necessary to allow proper accountability and

traceability of product

• Maintains and keep inventories of all required equipment, materials, supplies and products

• Demonstrates proficiency with Distributed Control System and other automated systems

(including, but not limited to, SAP, MES, NG LIMS, HanDEL, BACH, Word, Excel)

• Performs general maintenance and assists in troubleshooting of equipment

• Performs re-feeding, inoculation and harvesting of virus working seed utilizing a robotic

operations

• Validation process for current process as well as developmental work for new products/processes

as required

• Identifies and addresses compliance, environmental, safety, and process deviations as appropriate

while notifying appropriate personnel

• Provides process ownership through timely actions

• Maintains, cleans and prepares equipment used in vaccine production.

• Schedules and performs environmental monitoring during processing and records results in the

computerized database system

• Provides timely delivery of sample and other materials as required to appropriate laboratories,

coordinating with Quality and Logistics as required

• Attends and actively participates in HAZOPS, waste walkthroughs, and Lean six-sigma, quality,

safety or environmental training/initiatives as required

• Adhere to 5S, kaizen events, or any other operations

Scientific Research Experience:

Graduate Research Assistant: North Carolina Central University, Biomanufacturing

Research Institute and Technology Enterprise (BRITE), Durham, NC, (January 2011 to December 2012 )

• Performed extensive work on transgenic tobacco plants

• Grew transgenic plants in greenhouse for transgene analysis and protein purification

• Extracted and purified human protein from transgenic plants

• Used different molecular techniques to confirm the presence of transgenes, to detect gene

expression levels and quantify accumulated protein levels

Summary of MS thesis:

Thesis Title: To improve the production of human asialo-erythropoietin (asialo-hEPO) in transgenic

tobacco plants

The aim of my thesis work is to improve the expression level of human asialo-erythropoietin (asialo-

hEPO) in transgenic tobacco plants. To achieve this, a genetic cassette containing human β1, 4-

galactosyltransferase gene (GalT) driven by GapC promoter and erythropoietin gene (EPO) by double

CaMV35S promoter was designed. EPO was engineered to have a Strep II tag and an endoplasmic

reticulum (ER) retention signal KDEL at the C-terminus to aid in the purification (Strep II tag) and

accumulation of higher levels of asialo-EPO in the ER (KDEL). Agrobacterium-mediated transformation

approach was employed to transfer an EPO + GalT genetic cassette into tobacco plants. Transgenic plants

were confirmed for the presence of EPO and GalT genes by PCR. Gene expression levels were measured

by RT-PCR and qRT-PCR. The protein product was detected by ELISA and western blotting. Future work

will purify more protein for its function studies and glycan mapping.

Laboratory Assistant (Internship): Alliance Hospital and William Clinic, Holly Springs, MS

(June 20007- August 2007)

Holly Springs, Mississippi

• Screened blood specimens using high powered ultra-dark field microscope.

• Performed daily cleaning inspection of all laboratory equipment.

• Ensured laboratory safety by following written procedures and protocols.

• Took responsible for detailing work completed in the laboratory notebook.

Research Assistant (internship): University of Mississippi, Oxford, MS (June 2006 - August 2006)

• Conducted a Nonmyeloablative mice research for the study of Immunology Stem Cell Transplant

for Leukemia treatment in the molecular Hematology and Radiotherapy lab.

• Shared the responsibilities of maintaining lab stocks and overseeing laboratory inventory for

supplies and equipment.

• Maintained Laboratory notebook detailing all test performed and data collected. Collaborated

with the professors and teammates, to ensure that all work completed in a timely fashion

• Collaborated with the professors and teammates, to ensure that all work completed in a timely

fashion

Other Work Experience

Quality Audit Technician

Flextronics, Memphis, TN (February 2007 - March

2009)

• Checked all components of laptop to make sure that the problem has been solved.

• Handled logging and reporting of computer repairs into the internal tracking system (data

base).

• Installed software packages to troubleshoot where necessary.

• Maintained reports and accurate logs of all computer inventories that entered and exited the

department.

• Worked directly with the bench technician and the shipping department on site to alleviate

technical problems and provide additional support.

Co- publisher of a scientific research paper in October, 2013:

For more detail, visit this website @ http://dx.plos.org/10.1371/journal.pone.0076468

Professional Affiliation:

Member of ISPE (International Society for Pharmaceutical Engineering) (Feb 2011 -

present)

References:

Available upon request.

Contact this candidate