Quality Control Assistant

Daniel Stewart

**********@*****.***

Mobile 919-***-****

Home Address

*** ***** ****** ***

Garner, NC 27529

OBJECTIVE

To obtain a position using my knowledge and experience working in a highly-

regulated, innovative biotechnology laboratory performing and developing

complex molecular biology assays with high integrity, writing and revising

standard operating procedures, as well as leading the training of new

laboratory staff in a team setting.

QUALIFICATIONS

. Motivated self-starter with meticulous attention to detail, excellent

time management, great communication skills, and an innovative approach

to problem solving

. Working as part of a team in a fast-paced laboratory environment

operating under GCLP, CLIA, and CAP guidelines

. Writing and revising SOPs to improve clarity and increase efficiency

. Performing installation, performance, and operational qualifications on

new technologies and instruments

. Training new staff in various laboratory procedures and ensuring that

they follow standard operating procedures

. Extracting DNA from blood, FFPE tissue, and buccal cells and quantifying

DNA using spectrophotometry and fluorospectrometry

. Extracting RNA from blood and FFPE tissue and analyzing using the Agilent

2100 bioanalyzer and performing cDNA synthesis

. Designing primers, performing polymerase chain reactions using the ABI

9700, and performing gel electrophoresis to genotype DNA using the ABI

7900 Sequence Detection System, the ABI 3130xL, and the ABI 3730xl

genetic analyzers

. Performing real-time PCR using the ABI 7900 Sequence Detection System and

the ABI Viia 7 Platform

. Performing microarray analysis using the DMET Plus array and the GeneChip

HTA 2.0 array

. Performing liquid transfers with the PerkinElmer Janus Automated Liquid

Handler

. Maintaining cell cultures and performing physiological experiments in

vitro

. Keeping a large amount of biological samples organized, and keeping a

detailed record in a laboratory notebook of experiments performed

. Maintaining laboratory equipment and ordering necessary supplies

. Knowledge of Microsoft Office, Vector NTI, Sequencher, Primer3, and the

NCBI website

RELEVANT EXPERIENCE

Gentris Corporation Morrisville, NC February 2010 - Present

Senior Clinical Services Technologist II

. Processes client samples using approved standard operating procedures

. Performs routine and non-routine intermediate to high complexity

pharmacogenomic assays in a GCLP, CLIA, and CAP regulated environment

including DNA purification and quantitation, gel electrophoresis, real-

time PCR using the ABI 7900 SDS and ABI Viia 7, and DNA Sequencing using

the ABI 3130xL and ABI 3730xL genetic analyzers

. Assists in identifying and troubleshooting discrepancies and quality

control failures

. Maintains equipment logs, maintenance logs, and calibration logs

. Assists in laboratory inventory, makes common reagents and reviews

reagent quality control

. Mentors and trains staff in the Clinical Services Technologist II

position

. Performs RNA extraction, cDNA synthesis and total RNA quality analysis

from a variety of sources including blood and FFPE tumor samples using

approved standard operating procedures

. Performs micoarray analysis using the Affymetrix DMET Plus array and the

GeneChip HTA 2.0 array

. Excellent skills in experimental design, implementation, troubleshooting,

and data analysis

. Absolute and/or relative quantification of gene expression levels (Chagas

parasite DNA) using real-time PCR methods on the ABI 7900 SDS and ABI

ViiA 7

. Review of gene expression data, gene expression protocols and final

reports

. Assists in the development and validation of new assays

. Assists in the writing, editing, and implementing standard operating

procedures, material requirements documents, and other essential

documentation

Cystic Fibrosis Center-Zariwala Lab Chapel Hill, NC April 2009 -

February 2010

Research Assistant

. Discovered a novel disease-causing mutation in DNAH5

. Extracting and isolating DNA from blood samples and buccal cells from

subjects with genetic lung disorders and family members

. Quantification of DNA via spectrophotometry using NanoDrop ND1000

. Whole genome amplification

. Sequencing and analyzing DNA samples using ABI 3130xl genetic analyzer

and Sequencher for use in the genetic diagnosis of primary ciliary

dyskinesia

. Storing and organizing biological samples in -80 centigrade freezers and

liquid nitrogen freezer tanks

. Keeping detailed records of patient samples and experiments performed

. Maintaining laboratory equipment

. Ordering necessary supplies

Cystic Fibrosis Center-Ostrowski Lab Chapel Hill, NC January 2008-March

2009

Research Assistant

. Worked on a project to sequence the candidate genes, hydin and klp1, to

search for possible primary ciliary dyskinesia-causing mutations

. Designed primers using Vector NTI to amplify exons using polymerase chain

reaction and gel electrophoresis

. Analysis of DNA sequence for possible mutations using Sequencher

. Searched scientific literature for possible candidate genes

. Performed physiological experiments in human airway epithelial cell

cultures, including helping to develop a protocol to measure nitric oxide

production in vitro

. Performed analysis of nitric oxide production in human airway epithelial

cell cultures to determine differences between cells from normal, cystic

fibrosis, and primary ciliary dyskinesia patients, and presented results

and conclusions to cilia research group meetings

. Kept detailed records of experiments performed

EDUCATION

University of North Carolina at Chapel Hill

B.S., Biology, Minor in Chemistry, May 2009

Undergraduate Research Project: Sequencing a candidate gene, hydin, for

possible mutations leading to primary ciliary dyskinesia; Paper and

presentation of results at Undergraduate Research group meetings, April

2008

Received full tuition grant by graduating from NC School of Science and

Mathematics

PUBLICATIONS

Ostrowski LE, Stewart D, Hazucha M. Interferon ? Stimulates Accumulation of

Gas Phase Nitric Oxide in Differentiated Cultures of Normal and Cystic

Fibrosis Airway Epithelial Cells. Lung. 2012. doi: 10.1007/s00408-012-9395-

7.

REFERENCES

Available upon request

Contact this candidate