Engineering Research
Resume:
CURRICULUM VITAE
Name: Yunlong He, Ph.D.
Email: ****@****.***.***
Work phone #: 301-***-****
Education:
**** *.*. (Biotechnology), Shandong University, Jinan, Shandong, China
**** **.*. (Molecular Microbiology), Shanghai Jiao Tong University, Shanghai, China
Brief Research Experience:
July, 2010-present: Visiting Fellow in Laboratory of Cancer Biology and Genetics, Center for
Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD
1. I generated a transcriptional factor (Tcf1) conditional knockout mouse Tcf1flox/flox. The
master regulator Tcf1 can be deleted in specific tissues or development stages. When
crossed with β-actin cre mice, the homozygous mice Tcf1-/- are partially embryonic
lethal (70% less than Mendel ratio). We did RNA-Seq in Tcf1flox/flox and Tcf1-/-
embryonic stem cells and found Tcf1 is a master regulator for fatty acid metabolism.
It’s also relevant to clear cell renal cell carcinoma (CCRCC). (ongoing work)
The lineage control role of p53 in bone marrow-derived mesenchymal stem cells
2.
(MSCs) and its relevance to osteosarcoma-genesis. My study found p53 inhibits
osteogenic differentiation of MSCs by repressing RunX2, a master regulator of
osteogenesis, but doesn’t affect adipogenic and chondrogenic differentiation. And the
repression was indirectly through miR34s. It indicates that p53 activity can influence
cell fate decision in MSCs, and provide molecular and cellular insight into the
observation that p53 loss is associated with increased osteosarcoma incidence.
(preparing for submission, 2014, first author)
3. To identify p53 targets in a global and unbiased manner, we performed gene expression
microarray and ChIP-chip assays using mouse embryonic fibroblasts (MEFs) and mouse
embryonic stem (mES) cells. We found a novel p53 downstream target, Rap2b which
promotes cell survival after DNA damage. (Cell Cycle, 2013, co-first author)
Yunlong He – CV and Bibliography
4. Whole-genome study of p53-mediated DNA damage signaling in mES cells reveal that
binding of p53 at the promoter region significantly correlates with gene activation but not
with repression. Unexpectedly, we identify a regulatory mode for p53-mediated repression
through interfering with distal enhancer activity. Importantly, many ES cell-enriched core
transcription factors are p53-repressed genes. (Mol Cell, 2012, second author)
2002-2010: Graduate student in Microbial Metabolism Laboratory at Shanghai Jiao Tong
University, Shanghai, China (Supervisor: Prof. Zixin Deng)
Cloning type I polyketide synthase (PKS) gene clusters of meilingmycin from Streptomyces
1.
nanchangensis. Our work provides a more specific strategy for the cloning of modular type
I PKS gene clusters by use of acyltransferase-ketoreductase didomain PCR amplification.
The cloning of the meilingmycin gene clusters paves the way for its pathway engineering.
(Appl Environ Microbiol, 2010, first author)
I constructed two efficient gene knockout and over-expression vectors for engineering in
2.
Streptomyces. (J Microbiol Biotechnol, 2010, first author)
Research Interests:
a. Biology: Biochemistry, Molecular Biology, Cell Biology.
b. Disease: Osteosarcoma and other cancer related research, Fatty acid metabolism disease.
c. Stem cells: Embryonic stem cells, Mesenchymal stem cells
Professional skills:
1. Laboratory research skills in Biochemistry, Molecular Biology, and Cell Biology
Have experiences in most of laboratory techniques of modern Molecular Biology, Biochemistry,
and Cell Biology as reflected from my peer-reviewed publications. These include, just to name a few,
Molecular cloning; Sequence analysis, annotation and functional characterization of DNA, RNA and
proteins; Expression and purification of recombinant proteins; Lentivirus or retrovirus based gene
knocking down or over-expression; Flow cytometry; Chip-Seq; RNA-Seq; Generation of cell culture
models and development of cell-based assays; Generation of genetically-modified animal models for
physiologic studies.
2. Embryonic Stem Cell Biology
I’m familiar with isolating and culturing mouse embryonic stem (ES) cells, reprogramming
Yunlong He – CV and Bibliography
somatic cells into induced pluripotent stem cells (iPSC), screening and validating targeted ES clones
for injection into blastocysts, and identifying mouse lines with germ-line transmission of the modified
genes.
3. Mesenchymal Stem Cell Biology
I have hands-on experience in mouse mesenchymal stem cells (MSCs), including isolating
MSCs from bone marrow by flow cytometry based cell sorting; culturing and differentiating MSCs
into different lineages in vivo and in vitro; and mouse injection for in vivo studies.
Awards and Honors:
1. Winner of Fellows Award for Research Excellence (FARE) 2013
2. Editorial Board for the Journal of Cancer Biology & Treatment (CBT) since 2014
3. Chief Judge for Stem Cells-General Section of FARE 2014 Travel Award Competition
4. Active Judge for Stress, Aging and Oxidative Stress/Free Radical Research Section of FARE
2015 Travel Award Competition
5. Associate Member of American Association for Cancer Research (AACR) since 2012
6. Active Member of Society of Chinese Bioscientists in America (SCBA) since 2012
Publications:
1. He Y, Dubios W, Yang H, Jiang S, Mishra P, Ren L, Gou H, Lal A, Khanna C, Merlino G, Lee M,
Huang J. p53 loss increases the heterogeneity and osteogenic differentiation of mesenchymal stem
cells. Stem cells. (undergoing review, 2014)
2. Shin MH, He Y, Huang J. Embryonic stem cells shed new light on the developmental roles of p53.
Cell & Bioscience. Oct 9, 2013, 3:42
3. Zhang X*, He Y*, Lee KH, Dubois W, Li Z, Wu X, Kovalchuk A, Zhang W and Huang J. Rap2b,
a novel p53 target, regulates p53-mediated pro-survival function. Cell Cycle. 12:8, 1279–1291;
April 15, 2013. (*co-first author)
4. Li M, He Y, Dubois W, Wu X, Shi J, Huang J. Distinct regulatory mechanisms and functions of
p53-activated and p53-repressed DNA damage response genes in embryonic stem cells.
Molecular Cell. 46(1):30-42. Apr 13, 2012.
Yunlong He – CV and Bibliography
5. Li M, He Y, Feng X, Huang J. Genome-wide studies of the transcriptional regulation by p53.
Biochim Biophys Acta, Feb 10, 2012.
6. He Y, Korboukh I, Jin J, Huang J. Targeting protein lysine methylation and demethylation in
cancers. Acta Biochim. Biophys. Sin. (Shanghai). 44: 70-9, 2012.
7. Liu F, Barsyte-Lovejoy D, Allali-Hassani A, He Y, Martin M, Chen X, Yates C, Frye S, Brown P,
Huang J, Vedadi M, Arrowsmith C, Jin J. Optimization of Cellular Activity of G9a Inhibitors
7-Aminoalkoxy-quinazolines. Journal of Medicinal Chemistry. 8: 6139, 2011.
8. He Y, Sun Y, Liu T, Zhou X, Bai L, Deng Z. Cloning of the separate meilingmycin biosynthetic
gene clusters through acyltransferase-ketoreductase (AT-KR) di-domain PCR amplification.
Applied and Environment Microbiology. 76(10): 3283-92, 2010.
9. He Y, Wang Z, Bai L, Liang J, Zhou X, Deng Z. Two pHZ1358-derivative vectors for efficient
gene knockout in Streptomyces. Journal of Microbiology and Biotechnology. 20(4): 678-82,
2010.
10. He Y, Zhu D, Bai L, Deng Z. Cloning of antibiotic biosynthetic gene clusters from genomic
library by narrow-down PCR. Journal of Shanghai Jiao Tong University. 43(1): 5-8, 2009.
11. Zhu D, He Y, Bai L, Deng Z. Positive regulation of avermectin biosynthesis by AveR in
Streptomyces avermitilis. Journal of Shanghai Jiao Tong University. 42(9): 1448-1452, 2008.
Contact information for references:
Stuart H. Yuspa, M.D.
Laboratory of Cancer Biology and Genetics
Head, In Vitro Pathogenesis Section
Laboratory Co-Chief
Center for Cancer Research
National Cancer Institute
Phone: 301-***-****
Fax:
E-Mail: *****@***.***
Yunlong He – CV and Bibliography
Curtis C. Harris, M.D.
Laboratory of Human Carcinogenesis
Head, Molecular Genetics and Carcinogenesis Section
Laboratory Chief
Center for Cancer Research
National Cancer Institute
Phone: 301-***-****
Fax:
E-Mail: *************@***.***
Beverly Mock, Ph.D.
Laboratory of Cancer Biology and Genetics
Head, Cancer Genetics Section
Deputy Laboratory Chief
Center for Cancer Research
National Cancer Institute
Phone: 301-***-****
Fax:
E-Mail: *****@****.***.***
Yunlong He – CV and Bibliography
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